Advanced Mammalian Cell Culture Techniques: Principles and Practices

By: K. M. Ramkumar and R. Senthilkumar and Md Enamul Hoque

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Synopsis: This up-to-date book compiles both basic and advanced laboratory techniques of mammalian cell culture. It is divided into four major sections encompassing the basics of cell culture, nucleic acid and protein isolation, cell-staining techniques, and cell transfection and single-cell analysis. The topics include aseptic handling, media preparation, and passaging of cells. The book also outlines downstream assays such as nucleic acid and protein isolation from in-vitro cell cultures. Key Features: • Covers cellular staining using fluorescent dyes, genetic manipulation of cells via transfection, and an introduction to single-cell analyses • Discusses basics in cell culture and downstream applications including gene and protein expression analysis • Includes the principles underlying each of the techniques and provides a detailed methodology to practice • Explores the whole range of techniques – from basic to downstream applications and advanced methods The book is essential for students and researchers in the field of life sciences, biotechnology, genetics, and molecular biology.

Book Quality:: Publisher Quality
ISBN-13:: 9781000969405
Related ISBNs:: 9781003397755, 9781032502854, 9781032494524
Publisher:: CRC Press
Date of Addition:: 10/25/23
Copyrighted By:: selection and editorial matter, K.M. Ramkumar, R. Senthilkumar, and Md Enamul Hoque
Adult content:: No
Language:: English
Has Image Descriptions:: No
Categories:: Nonfiction, Science, Animals
Submitted By:: Bookshare Staff
Usage Restrictions:: This is a copyrighted book.
Edited by:: K.M. Ramkumar
Edited by:: R. Senthilkumar
Edited by:: Md Enamul Hoque