Ocular Regeneration: Methods and Protocols (2025) (Methods in Molecular Biology #2848)

By: Jeffrey M. Gross

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Synopsis: This detailed volume presents state-of-the-art techniques in ocular regeneration research, pulling together recent animal model systems identifying key genes and regulatory networks that contribute to the regenerative response as well as advances in induced pluripotent stem cell (iPSC) biology. The book opens with a section on the anterior segment and key models and techniques utilized to study the cornea, lens, and trabecular meshwork, and continues with chapters on the posterior segment, and largely the retina and retinal pigment epithelium (RPE), with techniques developed in a variety of model systems to study regeneration of these tissues. The concluding part examines novel technologies utilized in ocular regeneration research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ocular Regeneration: Methods and Protocols serves as an ideal guide for researchers aiming to expand the boundaries of what we know about this vital area of physiology.