Pulsed-Field Gel Electrophoresis

By: Levy Ulanovsky and Margit Burmeister

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Synopsis: Burmeister and Ulanovsky's new volume on Pulsed-Field Gel Electrophoresis presents step-by-step protocols for all users of this powerful methodology, whether novice or expert. The book features a wide range of PFGE techniques, auxiliary methods, and a diverse array of powerful applications. Each protocol is given thorough treatment by authors who have had extensive hands-on-experience with it. Indeed, many of the contributors have long provided assistance and troubleshooting advice to new users of PFGE during the early stages of its growth. Here they incorporate the benefits of their experience in the extensive Notes sections, which describe common problems and pitfalls and offer sensible solutions for dealing with them. Topics and techniques treated include: * FIGE, CHEF, OFAGE, TAFE, FORE, ODPFGE * preparation of DNA suitable for PFGE from: bacteria, protozoa, plants, mammals, and yeasts. * preparation and analysis of yeast artificial chromosomes (YACS) * mapping strategies * biological applications * theories and observations. In keeping with the well-known practicality of Humana's Methods in Molecular Biology series, Pulsed-Field Gel Electrophoresis brims with protocols designed to work even the first time they are used.