PCR Protocols
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- Synopsis
- The newest and most up-to-date collection, PCR Protocols offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. Although specific examples and experimental systems are described, the book's focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers. PCR Protocols features clear, easy-to-follow descriptions of procedures and a Notes section in each chapter to provide tips, alternative suggestions, and other enhancements of the protocols. Key Topics and Techniques: basic startup procedures for PCR * PCR in detecting DNA and RNA * PCR in DNA synthesis and mutagenesis * PCR cloning of DNA * selection of primers * radioactive and nonradioactive labeling * quantitation of PCR products by HPLC * chromosome assignment * PCR mapping of HLA genes * sequencing of PCR products * genomic footprinting * quantitation of tumor gene expression * screening of phage libraries * SSP-PCR and genome walking * subcloning of PCR products.
- Copyright:
- 1993
Book Details
- Book Quality:
- Publisher Quality
- ISBN-13:
- 9781592595020
- Related ISBNs:
- 9780896032446
- Publisher:
- Humana Press, Totowa, NJ
- Date of Addition:
- 06/02/13
- Copyrighted By:
- Humana Press, Totowa, NJ
- Adult content:
- No
- Language:
- English
- Has Image Descriptions:
- No
- Categories:
- Nonfiction, Science
- Submitted By:
- Bookshare Staff
- Usage Restrictions:
- This is a copyrighted book.