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Showing 31,126 through 31,150 of 34,547 results

Transgenic Plants

by Leandro Peña

A collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative real-time PCR, RT-PCR, in situ hybridization, FISH, and TAIL-PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Transgenic Plants

by Andy C Wetten Jim M. Dunwell

In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edition expert researchers in the field provide key techniques to investigate production and analysis of transgenic plants. Focusing on selection and detection methods, transformation technology, gene targeting, silencing and directed mutation, metabolic engineering and pharming, the book encompasses protocols relating to major crops and model plants being used for genomic analysis. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Transgenic Plants: Methods and Protocols, Second Edition aids scientists in the continuous improvements being made for the production and analysis of transgenic plants.

Transgenic Wheat, Barley and Oats

by Huw D. Jones Peter R. Shewry

The understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture. In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocols for the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, in order to fill the need for techniques and resources in this vital area of research. Covering such topics as in vitro Agrobacterium co-cultivation, the manipulation of gene expression, and GM risk assessment, this versatile collection focuses on the study of temperate small grain cereals while many of its readily reproducible procedures could be easily adapted to accommodate other cereals and plants. The in-depth chapters adhere to the highly successful Methods in Molecular BiologyTM series format, featuring brief introductions to the subject, lists of the necessary materials and reagents, step-by-step laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Transgenic Wheat, Barley and Oats: Production and Characterization Protocols is an ideal guide for scientists pursuing this powerful, forward-thinking genetic strategy.

Transhumanism and Society

by Stephen Lilley

This book provides an introductory overview to the social debate over enhancement technologies with an overview of the transhumanists' call to bypass human nature and conservationists' argument in defense of it. The author present this controversy as it unfolds in the contest between transhumanists proponents and conservationists, who push back with an argument to conserve human nature and to ban enhancement technologies. This book provides an overview of the key contested points and present the debate in an orderly, constructive fashion. Readers are informed about the discussion over humanism, the tension between science and religion, and the interpretation of socio-technological revolutions; and are invited to make up their own mind about one of the most challenging topics concerning the social and ethical implications of technological advancements.

Transitions Between Sexual Systems: Understanding the Mechanisms of, and Pathways Between, Dioecy, Hermaphroditism and Other Sexual Systems

by Janet L. Leonard

This book focuses on explaining the distribution of sexual systems (simultaneous hermaphroditism, sequential hermaphroditism, environmental sex determination,dioecy, androdioecy, etc.) among taxa, which remains a major challenge in evolutionary biology. Although significant advances have been made for angiosperms, there is not yet a theory that predicts the sexual system for the majority of animal taxa, and other taxa of plants also remain poorly understood. The problem, particularly for animals, is that sexual systems can be very conservative, with whole phyla and classes being characterized by a single sexual system; for example essentially the whole phylum Platyhelminthes is simultaneously hermaphroditic, whereas the Insecta (Hexapoda) and the Tetrapoda among the vertebrates, are exclusively dioecious. Sex allocation theory on the other hand, suggests that sexual systems should be highly responsive to evolution, changing with population density, life span, patterns of resource availability, etc. The book provides an overview of the topic and then presents a series of chapters, each dealing with a taxon with substantial lability in sexual system in order to identify the factors associated with changes in sexual system in each case. By doing so, the authors reveal factors that have not been considered in formal theory but seem to have a major impact on transitions between sexual systems.This book appeals to a wide readership in fields from zoology and evolutionary biology to botany.

Translational Stroke Research

by Paul A. Lapchak John H. Zhang

This volume sets a basis for effective translational research. Authored by experts in the field of translational stroke research, each chapter specifically addresses one or more components of preclinical stroke research. The emphasis is placed on target identification and drug development using state-of-the-art in vitro and in vivo assays, in combination with in vitro toxicology assays, AMDE and clinical design.

The Transmogrification of Roscoe Wizzle

by David Elliott

"The wacky plot and quirky details ... will appeal to young and reluctant readers who like their fiction light and offbeat."-- School Library Journal. Roscoe Wizzle used to be a normal ten-year-old kid. But that was before a sign reading Coming soon! GUSSY'S! sprang up in a vacant lot. A sign showing Gussy Gorilla eating a Jungle Drum--just about the biggest hamburger in the world. Roscoe Wizzle, hamburger fan, was a normal kid all right until he started turning into a bug! David Elliott's debut novel takes a hilarious and surreal look at what can happen when you get too much of a good thing.

Transylvanian Dinosaurs

by Coralia-Maria Jianu David B Weishampel

The history and science of a cluster of dinosaurs found in the Hungarian region and the story of the aristocrat who discovered them.At the end of the time of the dinosaurs, Transylvania was an island in what was to become southeastern Europe. The island’s limited resources affected the size and life histories of its animals, resulting in a local dwarfism. For example, sauropods found on the island measured only six meters long, while their cousins elsewhere grew up to five times larger. Here, David B. Weishampel and Coralia-Maria Jianu present unique evolutionary interpretations of this phenomenon.The authors bring together the latest information on the fauna, flora, geology, and paleogeography of the region, casting these ancient reptiles in their phylogenetic, paleoecological, and evolutionary contexts. What the authors find is that Transylvanian dinosaurs experienced a range of unpredictable successes as they evolved.Woven throughout the detailed history and science of these diminutive dinosaurs is the fascinating story of the man who first discovered them, the mysterious twentieth-century paleontologist Franz Baron Nopcsa, whose name is synonymous with Transylvanian dinosaurs. Hailed by some as the father of paleobiology, it was Nopcsa alone who understood the importance of the dinosaur discoveries in Transylvania; their story cannot be told without recounting his.Transylvanian Dinosaurs strikes an engaging balance between biography and scientific treatise and is sure to capture the imagination of professional paleontologists and amateur dinophiles alike.“It is rare to find a book on dinosaurs so literate, well-written, and full of insight and synthesis—particularly when the dinosaurs are so unusual. The authors lay them out for us, situate them beautifully in time, space, and cultural history, and then reassemble them and their world using all the tools of modern science. The result is a tour de force.” —Kevin Padian, University of California Museum of Paleontology“A fine example of something I always try, but rarely succeed, to articulate to colleagues in paleontology, evolutionary biology, and geology who don’t work on dinosaurs. Dinosaurs, within the context of their ecosystems and paleogeography, can tell us many neat things about how evolution works over long time scales.” —Stephen Brusatte, Priscum

Transylvanian Dinosaurs

by David B. Weishampel Coralia-Maria Jianu

At the end of the time of the dinosaurs, Transylvania was an island in what was to become southeastern Europe. The island's limited resources affected the size and life histories of its animals, resulting in a local dwarfism. For example, sauropods found on the island measured only six meters long, while their cousins elsewhere grew up to five times larger. Here, David B. Weishampel and Coralia-Maria Jianu present unique evolutionary interpretations of this phenomenon. The authors bring together the latest information on the fauna, flora, geology, and paleogeography of the region, casting these ancient reptiles in their phylogenetic, paleoecological, and evolutionary contexts. What the authors find is that Transylvanian dinosaurs experienced a range of unpredictable successes as they evolved.Woven throughout the detailed history and science of these diminutive dinosaurs is the fascinating story of the man who first discovered them, the mysterious twentieth-century paleontologist Franz Baron Nopcsa, whose name is synonymous with Transylvanian dinosaurs. Hailed by some as the father of paleobiology, it was Nopcsa alone who understood the importance of the dinosaur discoveries in Transylvania; their story cannot be told without recounting his.Transylvanian Dinosaurs strikes an engaging balance between biography and scientific treatise and is sure to capture the imagination of professional paleontologists and amateur dinophiles alike.

The Trapdoor (Fountas & Pinnell Classroom, Guided Reading)

by Jacqueline Adams

NIMAC-sourced textbook. Sneaky Spider. A hunter sets a trap and waits. The hunter is a spider, and it is waiting for its next meal to come along. But it has a very sneaky way of catching its meals.

Trapped: Brenna (Vet Volunteers #8)

by Laurie Halse Anderson

Someone's been setting animal traps in the nature preserve behind Brenna's house. She and her older brother, Sage, are furious not only is that illegal, it's hurting and killing defenseless animals where they should be safe. They both want to do something, but Brenna's worried that Sage may have some really extreme plans in mind. Can she rein him in?

Trapped #8

by Laurie Anderson

Someone's been setting animal traps in the nature preserve behind Brenna's house. She and her older brother, Sage, are furious - not only is that illegal, it's hurting and killing defenseless animals where they should be safe. They both want to do something, but Brenna's worried that Sage may have some really extreme plans in mind. Can she rein him in?

Trapped by a Teacher

by Dr Mary Ann Duke

MEET MRS. BATTLES, a fifth grade teacher with a thing or two to teach her spirited students. Mrs. Battles doesn't spend her time after school with home visits and meetings; instead, she spends her time trapping wild alligators for the Department of Fish and Game. A real-life gator trapper for a teacher? Victoria Scarlett Jones and her friends learn that sometimes the most adventurous part about school can be your teacher!

Trapped in Yosemite

by Dana Mentink

When a massive earthquake levels the region, secrets and unknown dangers are exposed…The ground in still-icy Yosemite National Park isn&’t stable, and K-9 handler Von Sharpe fears the worst. But his worries are pushed aside when he sees an SUV viciously forced off the road…with his ex-fiancée at the wheel. It&’s only when Stella Rivers steps out of the vehicle that the very ground under their feet cracks and breaks with the onslaught of a terrifying earthquake. When her meeting with a mysterious client is ambushed, Stella witnesses the man being gunned down by a sniper and becomes a target herself. With the threat of aftershocks triggering landslides and floods, the new mom has no choice but to run to save her baby girl—even if it means teaming up with the man who has no idea he fathered her child.Trapped in a dangerously unstable wilderness with a killer closing in, Von and Stella are running out of time to get to safety. And every fissure reveals a new secret that will shake them to their foundation…

Trapped! (Pete The Cat)

by Peg Kehret

A new Pete the Cat mystery in paperback for the first time! Alex and his friends witness a pig falling from a speeding red truck. Working with the police and an animal rescue group, the kids get permission to keep the pig. But after a local TV station runs a story about the rescue, the angry truck driver shows up at Alex?s house, determined to take the pig to slaughter. Who is this man? What is he hiding? Pete uses his skills to solve the mystery but, as usual, his humans don?t understand. When the dangerous situation turns deadly, can Pete save himself?

Trapped! A Whale's Rescue: A Whale's Rescue (Live Oak Media Ereadalong Ser.)

by Robert Burleigh

In the icy waters of the Pacific, a massive humpback whale unexpectedly finds herself tangled in a net abandoned by fishermen. When a rescue boat and a convoy of divers arrive to help the struggling humpback, a realistic and moving encounter bridges the human and aquatic worlds.

Trapping 101: A Complete Guide to Taking Furbearing Animals

by Philip Massaro

Tips, tactics, and techniques for all skill levels. The ancient art of trapping goes back centuries, almost to the beginning of civilization. Native Americans used the pit trap, deadfalls, and snares, the Chinese documented the use of nets and pits in the fourth century BCE, and virtually every civilization can exhibit some example of the use of a trap in one form or another to procure meat, hides, or fur. The fur trade across Europe was dominated by the Russians, which provided furs to the greater part of Western Europe and Asia during the Middle Ages, which prompted the exploration of Siberia and its game rich forests. In North America, trapping was one of the primary reasons why settlers pushed West, taking advantage of the bountiful game across the continent. Fur was used not only for coats, hats, and mittens, it was used as a form of barter. The taking of a fur-bearing animal was and is a big accomplishment, as fooling a crafty animal on its home territory is no easy feat. In Trapping 101, veteran trapper Phil Massaro reveals all the secrets of the trade, from knowing where to set traps, to understanding and using various types of traps, to properly using scents. Tips and tactics for taking beavers, muskrats, weasels, raccoons, skunks, otters, and more are all covered. While there is a wealth of information in here for beginners, information that will help them pick up trapping with relative ease, there are many subtle tips and tricks that even a veteran trapper will appreciate. Times have, of course, changed since the days of the voyageurs and rendezvouses. There are many more people in this modern world, many more dwellings, many more towns and cities. But there is a place for trapping in all this, just as there are places for hunting and fishing. A knowledgeable trapper, following game rules and respecting the animals he is trying to trap, fits right into the grand scheme of Mother Nature existing in harmony with humankind. This book will help you achieve that.

Trapping and the Detection, Control, and Regulation of Tephritid Fruit Flies

by Todd Shelly Nancy Epsky Eric B. Jang Jesus Reyes-Flores Roger Vargas

The book focuses on four broad topics related to trapping of agriculturally important tephritid fruit flies, namely i) lures and traps, ii) invasion biology and detection of infestations, iii) attract and kill systems, and iv) trade regulations and risk assessment. This comprehensive structure progresses from the biological interaction between insect and lures/traps to the area-wide use of trapping systems to the utilization and impact of trapping data on international trade. The chapters include accounts of earlier research but are not simply compendia and instead evaluate past and current work as a tool for critical analysis and proposal of productive avenues for future work. At present there is no book available that deals with fruit fly trapping in such a broad context. Our book fills this gap and serves as a global reference for both those interested in fruit flies specifically as well as anyone dealing with the threat of invasive agricultural insects in general.

Trash Animals: How We Live with Nature’s Filthy, Feral, Invasive, and Unwanted Species

by Kelsi Nagy Phillip David Johnson II

Why are some species admired or beloved while others are despised? An eagle or hawk circling overhead inspires awe while urban pigeons shuffling underfoot are kicked away in revulsion. Fly fishermen consider carp an unwelcome trash fish, even though the trout they hope to catch are often equally non-native. Wolves and coyotes are feared and hunted in numbers wildly disproportionate to the dangers they pose to humans and livestock. In Trash Animals, a diverse group of environmental writers explores the natural history of wildlife species deemed filthy, unwanted, invasive, or worthless, highlighting the vexed relationship humans have with such creatures. Each essay focuses on a so-called trash species—gulls, coyotes, carp, cockroaches, magpies, prairie dogs, and lubber grasshoppers, among others—examining the biology and behavior of each in contrast to the assumptions widely held about them. Identifying such animals as trash tells us nothing about problematic wildlife but rather reveals more about human expectations of, and frustrations with, the natural world. By establishing the unique place that maligned species occupy in the contemporary landscape and in our imagination, the contributors challenge us to look closely at these animals, to reimagine our ethics of engagement with such wildlife, and to question the violence with which we treat them. Perhaps our attitudes reveal more about humans than they do about the animals. Contributors: Bruce Barcott; Charles Bergman, Pacific Lutheran U; James E. Bishop, Young Harris College; Andrew D. Blechman; Michael P. Branch, U of Nevada, Reno; Lisa Couturier; Carolyn Kraus, U of Michigan–Dearborn; Jeffrey A. Lockwood, U of Wyoming; Kyhl Lyndgaard, Marlboro College; Charles Mitchell, Elmira College; Kathleen D. Moore, Oregon State U; Catherine Puckett; Bernard Quetchenbach, Montana State U, Billings; Christina Robertson, U of Nevada, Reno; Gavan P. L. Watson, U of Guelph, Ontario, Canada.

Trash Mountain

by Jane Yolen

This you should know: Gray squirrels are almost always larger, faster, and more aggressive than reds. They out-eat the reds and out-breed them. Science says the grays will eventually win. Nutley is a young red squirrel. For most of his life, he's been content to live on local seeds and the cautious wisdom of his parents. But like so many young squirrels before him, he feels the call of the wild (and the hazelnuts) beyond the safety of his family's own tree. Nutley wonders what it would be like to be Dangerous, like the growing band of gray squirrels that roam his neighborhood. Nature, which is truly red in tooth and claw, forces Nutley to find out if he's cut out for a life of danger. He must flee his familiar tree for the smelly shelter of the local landfill. There, with the help of some unlikely allies, he might just be able to make a stand against the grays. This you should know: No matter what scientists say is almost always true, the exceptions are almost always the best stories.

Travel Wild Wisconsin

by Candice Gaukel Andrews

Have you ever heard a wolf howl in Wisconsin's Northwoods, watched thousands of ancient sturgeon roil the waters of one of the largest inland lakes in the United States, or tagged a monarch butterfly before it begins one of the world's great migrations, to its winter habitat in Mexico?Travel Wild Wisconsinis your seasonal guide to genuine wildlife encounters with an amazing array of birds, mammals, fish, and insects in Wisconsin's most beautiful natural settings: state wildlife areas, rivers, lakes, flowages, and preserves as well as national wildlife refuges and forests. Wisconsin native Candice Gaukel Andrews shares natural history and lore, accounts of her own experiences with Wisconsin wildlife, and insights from biologists, environmental educators, and citizen scientists, so that you can seek a wildlife encounter of your own. So come spy on the spring courtship dance of the greater prairie chicken, search for elusive and elegant white-tailed deer in summer, touch a tiny saw-whet owl on one special day in autumn, and thrill to the sound of thousands of tundra swans as they migrate through the Mississippi Flyway just before the first snow falls. Make this the year youTravel Wild Wisconsin.

The Travelers and the Bear (Into Reading, Level N #68)

by Lynne Benton Aleksey Olga Ivanov

NIMAC-sourced textbook

Traveling Blind: Adventures in Vision with a Guide Dog by My Side

by Susan Krieger

Krieger (feminist studies, Stanford U.) recounts her experiences as a newly blind person learning to navigate with her guide dog.

Traveling Butterflies

by Susumu Shingu

Traveling Butterflies indulges monarch’s life cycle, progression from an egg the size of a dewdrop through growth, metamorphosis, and preparation for their journey south. Illustrations in a vibrant color palette bring the butterflies to life and depict the rural and urban landscapes through which they fly and factual text about monarchs and their migration.

Traveling Rose

by Brian Wray

Award-winning author-and-illustrator team Brian Wray and Shiloh Penfield are back with a new adventure featuring their beloved children's book character, Rose the stuffed bunny. In Traveling Rose, she is taking her very first big trip to London with her friend Iris and her favorite little boy. Rose is excited about the teas they would have, but soon becomes distracted by her worries. Did she pack her favorite sweater in case the weather is cold? Did she remember to turn off the lamp by her bedside? Her thoughts feel so big that Rose begins to cry and no longer wants to go. Can Rose find a way to let her worries at home and have fun on her trip? The story offers a great way for parents and teachers to talk to children about what happens when impulsive and obsessive thoughts get in the way of enjoying life's big moments as well as everyday life, and what to do about it. These types of “first times” can be stressful for anyone but, for someone with intrusive thoughts, can be filled with obstacles. Intrusive worries are there at every turn, pointing out all of the things that could go wrong or that are “dangerous.” It takes work to see through the fears and picture the possibilities, especially for our beloved Rose Bunny. Children will identify with Rose, and by allowing children to see themselves in a story, it shows them that they are not alone in their experience. Ideal for social and emotional learning, Traveling Rose teaches children to express emotion, which is one of the key ingredients to healthful development. Rose continues to be a starting point to having larger conversations that will create a lasting impact. Don’t miss her in her first picture book, Unraveling Rose.

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