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Zebrafish
by Michael Orger E. Elizabeth Patton Koichi KawakamiThissecond edition details new emerging areas of zebrafish research focusing on geneticsand genomics, techniques for developing and analyzing zebrafish disease models,and methods for neuroscience. Zebrafish:Methods and Protocols, Second Edition guides readers through methods for mutagenesisand genome editing in zebrafish, applicationsof GFP-expressing transgenic fish, techniques for cancer models, imaging of infectionand host-pathogen interactions, metabolism and transport of lipids, and the structure and function of neural circuitsand their role in generating behavior. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls. Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a usefulcomplement to the first book for new and experienced zebrafish researcheralike.
Zebrafish: A Model for Marine Peptide Based Drug Screening
by Saravanan Ramachandran Senthilkumar RajagopalThis book offers a comprehensive overview of toxicology, highlighting the significance of peptide-based toxins from marine environments. It discusses the principles of protein-carbohydrate and domain-domain interactions to increase our understanding of toxicology in zebrafish models, as well as drug interaction mechanisms and target definition in drug discovery. It also reviews the structure of marine peptides/toxins and the toxicology of peptide secreting cells and cells that respond to these enzymes, and describes the normal and abnormal toxicology of marine peptides in zebrafish models. Offering insights into the field of proteomics, particularly current practice and research models for solving its many riddles, the book also explains the analytical principles of marine protein-protein and protein-carbohydrate interaction in the context of teratogenicity in target identification in peptide- based drug discovery. Lastly, the book methodically examines the preclinical research on marine proteins/peptides.
Zebrafish Models in Neurobehavioral Research
by Allan V. Kalueff Jonathan M. CachatAnimal models have traditionally played a crucial role in improving our understanding of brain pathogenesis. Zebrafish (Danio rerio) have generated considerable discoveries in the areas of genetics, embryology, endocrinology, and neuroscience. Zebrafish Models in Neurobehavioral Research emphasizes the growing importance of zebrafish in neurobehavioral research and portrays an extensive, thorough perspective on the emergence of zebrafish as robust and translational models. Written by leading international experts, the book covers major topics ranging from stress to learned recognition of environment, encompassing a wide spectrum of the utility of zebrafish within neurobiological disciplines. The chapters provide authoritative reviews of many zebrafish paradigms commonly used in the field today. This book will be a useful guide for zebrafish researchers, and will complement another related book from the popular Neuromethods series, Zebrafish Neurobehavioral Protocols. Comprehensive and up-to-date, Zebrafish Models in Neurobehavioral Research serves as an ideal resource for scientists new to the field as well as for established researchers seeking valuable insight into the growing utility of zebrafish in neuroscience.
Zebrafish Neurobehavioral Protocols
by Jonathan M. Cachat Allan V. KalueffAs a model organism, zebrafish (Danio rerio) offer an opportunity to perform steadfast scientific investigations in a robust and high-throughput manner. In Zebrafish Neurobehavioral Protocols, experts in the field provide a series of detailed behavioral protocols dealing with the major current research areas in the field of behavioral neuroscience. The chapters discuss the use and interpretation of video-aided quantification of zebrafish behaviors, describe novel assays commonly used to quantify emotionality, learning, memory, and social behaviors in zebrafish, and instruct how to quantify endocrine responses and correlate them with behavior. Written in the Neuromethods series format, the chapters provide authoritative reviews of many commonly used approaches in the field today. This "how to" book will be a useful practical guide for zebrafish researchers and will complement another related book from this series, Zebrafish Models in Neurobehavioral Research. Cutting-edge and timely, Zebrafish Neurobehavioral Protocols serves as an excellent compilation epitomizing the growing potential of zebrafish in modern scientific inquiry.
Zebrafish Protocols for Neurobehavioral Research
by Adam Michael Stewart Allan V. KalueffZebrafish (Danio rerio) play an integral role in biomedical research, enabling researchers to examine physiological mechanisms and pathways relevant to human pathogenesis and its therapy. That, along with their low cost, easy manipulation, short reproductive cycles, and physiological homology to humans, has made zebrafish a vital model organism for neuroscience research. Zebrafish Protocols for Neurobehavioral Research addresses protocols for both larval and adult models, written by the leading experts in the field of zebrafish research. Part I of this book takes advantage of the high-throughput nature of larval models to offer protocols for research requiring high output, easily manipulated screens. The second half of the book focuses on the robust and sophisticated behaviors of adult zebrafish, suitable for the neurophenotyping of complex traits and multi-domain disorders. Importantly, these models complement each other, working together to provide researchers with valuable insights into neurobiology of normal and pathological behavior. Thorough and cutting-edge, this volume is a useful, authoritative reference guide that should hold a coveted spot in zebrafish laboratories across the globe.
Zebras / Cebras
by Amelie Von Zumbusch Pilar SanzSimple bilingual English/Spanish text will captivate English language learners as they focus on the habitats, behaviors, and physical adaptations of the zebra.
Zebras (Nature's Children)
by Bill IvyHow many kinds of Zebras are there? How fast can a zebra run? Why does a zebra have stripes? Find the answers to these questions and more in this book about the physical characteristics, behavior, distribution, and life of zebras.
Zeke Takes a Bath
by Carrie WatersRead this story about an elephant on a farm. Ask yourself questions about what he and the other animals are trying to do.
Zelda and Ivy: The Runaways (Fountas & Pinnell LLI Blue #Level J)
by Laura KvasnoskyThe fabulous fox sisters return in three fresh, funny stories perfect for beginning readers. Whether Zelda and Ivy are combating the injustice of cucumber sandwiches for lunch, deciding what to leave the children of the future in their time capsule, or solving Zelda's writer's block, they are sure to tickle the funny bones of both new and returning fans.
Zelda and Ivy: Keeping Secrets (Fountas & Pinnell LLI Blue #Level K)
by Laura KvasnoskyA brand-new companion to the Theodor Seuss Geisel Award winner! The fabulous fox sisters return in three wry adventures for beginning readers. <P><P>It’s springtime, and little sister Ivy wants to prove she can keep a secret, but there are some secrets that are too good to keep. Big sister Zelda longs to play the perfect April Fool’s Day prank, but can she outsmart the clever Ivy? And will opera star hopeful Zelda be upstaged by a butterfly in the final act? Fresh and funny, full of sugar and sass, this trio of tales about everyday life with siblings and friends is sure to strike a chord with young readers everywhere.
Zelda and Ivy
by Laura Mcgee KvasnoskyStories of two inventive sisters at play, and at odds, ZELDA AND IVY is packed with sugar and sass -- a first-rate original! Zelda and Ivy are fox sisters with a flair for the dramatic. Their exploits unfold with plenty of sugar and sass in this spirited trio of stories.
Zelda and Ivy: The Big Picture
by Laura Mcgee KvasnoskyIn a fresh and funny new companion to the Theodor Seuss Geisel Award winner, the fox sisters team up with their friend Eugene in three spy-rific adventures. After Zelda, Ivy, and their best friend Eugene watch the new Secret Agent Fox movie (some more bravely than others), they are inspired to do a little detective work themselves. With top-secret agent names chosen and notebook in hand, they're ready to get to the bottom of neighborhood mysteries. But when a rainstorm threatens to ruin the agents' campout, can the three foxy friends come up with an undercover alternative? Sly humor, lively illustrations, and a genuine eye for the everyday dynamics among siblings make this a perfect choice for beginning readers, whether old fans or new. From the Hardcover edition.
Zelda and Ivy, the Runaways
by Laura Mcgee KvasnoskyIn three short notes, two fox sisters run away from home, bury a time capsule, and take advantage of some creative juice.<P><P> Winner of the Theodore Seuss Geisel Medal
Zelda's Big Adventure
by Marie Alafaci Shane McGZelda has big plans—she wants to be the first chicken in space. She leaves nothing to chance as she builds her spaceship, plans her experiments, and packs for the journey. All she needs now is a little help from her friends, and if they won’t step up, Zelda will just have to manage on her own! Eye-popping art and a story of perseverance and ingenuity prove that for a plucky hen, the sky is never the limit.This modern twist on the traditional The Little Red Hen story features an endearing and resourceful chicken determined to fulfill her dream.
Zelf kippen houden - Alles wat je moet weten over de verzorging van kuikens tot leggende kippen
by Isaac Miller Dick StadaZelf kippen houden is veel makkelijker dan je denkt en simpeler dan het verzorgen van de gebruikelijke huisdieren. Met een paar minuten aandacht per dag heb je zomaar elke dag verse eieren. In dit boek bekijken we welke stappen je moet nemen. Van het aanschaffen van kuikens tot het overbrengen van ze naar het buitenhok en alles wat je in de tussentijd kunt verwachten. Pak wat te eten en te drinken, zoek een lekker plekje om te zitten en ontdek in dit boek hoeveel plezier je van je eigen kippen kan hebben. In dit boek behandelen we onder andere: Benodigde ruimte en afmetingen van het hok Een ras kiezen Verzorging van de kippen Wanneer kunnen ze naar het buitenhok Schoonhouden van het kippenhok Hoe voer je de kippen Ik wens je veel plezier met dit boek en je nieuwe vriendjes.
Zen Cat
by Judith AdlerHow often have we seen cats watching us with intense focus, playing obsessively with a toy, comforting us as they cannily assess our moods, lying calmly in a patch of sunlight? Cats exemplify the essential tenets of Zen philosophy and the desire to find the simple and spiritual in everyday life. They embody utter grace, nonjudgmental devotion, and a sense of spontaneous whimsy. What's more, as we interact with cats, these qualities are reflected back in us.Zen Cat pairs more than 50 charming black-and-white photographs of cats with simple and inspiring quotes from a variety of sources: A fat cat lying on the floor evokes the statement "A hand-rolled dumpling of heaven and earth; I gulped it down and easily it went"; a picture of a cat up for adoption is accompanied by Buddha's question "If we fail to look after others when they need help, who will look after us?" This ingenious coupling of concepts and images reminds us that even the most finicky of our feline friends have a lot to teach us, if only we take the time to learn.
Zen Dogs
by Alex CearnsAward-winning animal photographer Alex Cearns celebrates the peace, calm, and joy dogs bring to our lives with this unique full-color collection capturing eighty dogs in their most relaxed and contented moments.When Alex Cearns caught Suzi the Sharpei on film with eyes closed and an endearing smile on her furry face, the renowned Australian professional pet and wildlife photographer called the picture of serenity "Zen Dog." Captivated by the image’s tranquil beauty, Cearns then turned her lens on other canines experiencing their own carefree and meditative "Zen" moments—a series of photos that would go viral across the web and take the top prize in a major international competition.Expanding on Cearns’s original web series, Zen Dogs includes eighty stunning color photographs of a variety of breeds—golden retrievers, beagles, French bulldogs, dachshunds, poodles, huskies, pit bulls, and German shepherds. Here are some familiar faces from the online series, joined by dozens of dogs never seen before—all whose engaging personalities shine through. Sprinkled throughout the photos are words of wisdom from the Buddha, Eckhart Tolle, Thich Nhat Hanh, and other meditative masters, inspiring messages that, with the photos, warm the heart and soothe the spirit.A gorgeous compendium for every dog lover, animal enthusiast, and everyone looking to add some peace and joy to their day, Zen Dogs reminds us of the power dogs have to enrich our lives—to make us happier, healthier, calmer, and more loving.
Zen Mind, Zen Horse: The Science and Spirituality of Working with Horses
by Allan J. Hamilton“Far more than a book about how to care for a horse, though it stands out as one of the best on the subject . . . beautiful” (Susan Richards, author of Chosen by a Horse). This unique guide to horsemanship incorporates Eastern philosophy to describe how horses understand and respond to the flow of vital energy around them, and how they use this energy, called chi, to communicate with their herd, express dominance, and sense predators. Written by the award-winning author of The Scalpel and the Soul, and including forewords by Monty Roberts and Dr. Robert Miller, Zen Mind, Zen Horse shares safe, simple techniques to make you more receptive to your animal’s chi, so you can develop a calm and effective training style that will not only help your horse follow commands, but strengthen the spiritual bond between horse and rider.
The Zen of Cat Walking: Leash Train Your Cat and Unleash Your Mind
by Clifford BrooksGoing for a walk is no longer just for the dogs! Experienced cat owner Clifford Brooks is here to teach you and your cat how to enjoy a stroll together. Cats are naturally adventurous creatures, and with the help of a leash and Brooks’s tips, you can indulge those instincts with a sense of security. As any animal trainer will tell you, the magic is in your attitude and sensitivity to your kitty’s needs and subtle reactions to new things. With that in mind, Brooks encourages cat owners to embrace Zen meditation as the foundation for leash training. Benefitting both you and your furry friend, practicing mediation will: • Provide a calm atmosphere in which to learn an enjoyable skill and explore new environments together. • Foster a positive energy between the two of you. • Cultivate patience and appreciation for simple accomplishments. With step-by-step instructions, meditation guidance, and interviews with other successful cat walkers, you’ll learn how cat walking can have a healthy impact on your life and the life of your feline companion. Essentially, you will become a more sensitive cat owner, able to detect when your own presence is affecting your cat’s behavior. Combining a practical skill with a thoughtful approach, Brooks’s advice will do wonders for the time you spend together.
The Zen of Home Water: True Tales of Adventure, Travel, and Fly Fishing
by Jerry HamzaA Collection of Fishing Stories from Across the Globe, by a Master Storyteller. To the uninitiated, it might be somewhat surprising to discover that fly fishermen tend to be rather contemplative sorts. During those dark nights and long seasons when fishing is not a promising endeavor, we settle down to the next best thing, reading our vast libraries of ancient fishing lore, interspersed with the odd philosophical tome. And when we do, we usually don&’t want to read proverbial stories about &“landing the big one,&” or lengthy how-to expositions on how to catch the aforementioned big one. Rather, we tend to prefer stories that place our beloved piscatorial pastime within the larger context of life and nature. Stories that, as Hamza describes, &“…sparks a light. A light that is both familiar and comforting.&” Such is The Zen of Home Water, the latest angling book by Jerry Hamza. Hamza is a John Volker for the new millennium. His book is interspersed with stories about monster brook trout, beautiful North woods streams and lakes, quirky backwoods guides, and legendary fly hatches. Through it all, he shows us one of the most profound truths of life, that &“It takes the acquisition of wisdom to understand that a happy life is actually a mosaic of small and insignificant events…we string together moments in life—like pearls becoming a beautiful necklace.&” The iridescent pearls that Hamza strings together are many and include the importance of &“freestyling&”, that uncontrollable escape impulse that implores us to drop whatever we are doing and head to the stream, any stream, with fly rod in hand. Another recurring theme is the need to unplug from the modern, electronic world. He instructs us how to trespass (with bartered permission) and fish those waters that look so inviting yet so out of reach to the (usually) law abiding. His recipe for squirrel stew is not jealously guarded but freely shared. And his stories of catching giant brook trout in the Maine North Woods allow the reader, who usually can&’t participate in such acts of angling greatness, to at least know that they are occurring to someone, somewhere. Hamza is a member of that peculiar subset of anglers, the bamboo rod aficionado. While acknowledging the cold, hard fact that bamboo rods are nothing more than conglomerations of &“expensive blades of grass,&” he also realizes that these handmade treasures passed down to us from previous generations will hopefully outlive us (and our car doors) and that we are merely their caretakers for a time. Although the dreaded &“g&” word (i.e., graphite) does make a brief appearance, Hamza is definitely one of those anglers who would rather hold an aged, organic creation of the bamboo rod maker&’s art than the latest admittedly efficient chemical concoction straight from the laboratory. This puts him squarely in the tradition of John Gierach, although Hamza&’s writing is better and his stories more entertaining. Hamza&’s own home waters are dual--Maine&’s Grand Lake Stream area and the southern shore of the Lake Ontario region. There are echoes of Thoreau&’s Maine Woods in his stories of remote lakes and plentiful trout. And while he takes us all around the country when relating his angling exploits (Kerouac&’s On the Road is a particular favorite of his), it is evident that the concept of &“home water&” carries a lot of weight with him. His beloved &“Zen Lake&”, with its less than perfect history and many small fish, could be the home water of any of us.
Zen Shorts
by Jon Muth"Michael," said Karl. "There's a really big bear in the backyard." This is how three children meet Stillwater, a giant panda who moves into the neighborhood and tells amazing tales. To Addy he tells a story about the value of material goods. To Michael he pushes the boundaries of good and bad. And to Karl he demonstrates what it means to hold on to frustration. With graceful art and simple stories that are filled with love and enlightenment, Jon Muth--and Stillwater the bear--present three ancient Zen tales that are sure to strike a chord in everyone they touch.
Zen Shorts (A Stillwater Book)
by Jon J MuthStillwater, the beloved Zen panda, now in his own Apple TV+ original series!This Caldecott Honor Book presents wondrous Zen tales to light up your life....When a giant panda named Stillwater moves into Michael, Addy, and Karl's neighborhood, he tells them the most amazing stories! To Addy, he tells a tale of a poor man who gives gifts to a robber. To Michael, he tells of a farmer who learns not to judge luck. And to Karl, he tells the tale of a monk who continues to carry the weight of a burden long past.With vibrant watercolors and elegant ink drawings, Jon J Muth--and Stillwater the bear--imaginatively present three classic Zen stories that abound with enlightenment and love.
Zen Socks (Stillwater)
by Jon J MuthStillwater, the beloved Zen panda, now in his own Apple TV+ original series!On life's journey...kindness is the key.Leo and Molly love their new neighborhood. Most of all they love their friend Stillwater. The three friends are quite a team! From Caldecott Honoree Jon J Muth comes a story about sharing, another about patience, and a third about compassion. With warmth and fun, they learn from one another in the most surprising ways....
Zenn Scarlett
by Christian SchoonZenn Scarlett is a bright, determined, occasionally a-little-too-smart-for-her-own-good 17-year-old girl training hard to become an exoveterinarian. That means she's specializing in the treatment of exotic alien life forms, mostly large and generally dangerous. Her novice year of training at the Ciscan Cloister Exovet Clinic on Mars will find her working with alien patients from whalehounds the size of a hay barn to a baby Kiran Sunkiller, a colossal floating creature that will grow up to carry a whole sky-city on its back.But after a series of inexplicable animal escapes from the school and other near-disasters, the Cloister is in real danger of being shut down by a group of alien-hating officials. If that happens, Zenn knows only too well the grim fate awaiting the creatures she loves.Now, she must unravel the baffling events plaguing her school, before someone is hurt or killed, before everything she cares about is ripped away from her and her family forever. To solve this mystery - and live to tell about it - Zenn will have to put her new exovet skills to work in ways she never imagined, and in the process learn just how powerful compassion and empathy can be.
Zenn Scarlett
by Christian SchoonZenn Scarlett is a bright, determined, occasionally a-little-too-smart-for-her-own-good 17-year-old girl training hard to become an exoveterinarian. That means she's specializing in the treatment of exotic alien life forms, mostly large and generally dangerous. Her novice year of training at the Ciscan Cloister Exovet Clinic on Mars will find her working with alien patients from whalehounds the size of a hay barn to a baby Kiran Sunkiller, a colossal floating creature that will grow up to carry a whole sky-city on its back.But after a series of inexplicable animal escapes from the school and other near-disasters, the Cloister is in real danger of being shut down by a group of alien-hating officials. If that happens, Zenn knows only too well the grim fate awaiting the creatures she loves.Now, she must unravel the baffling events plaguing her school, before someone is hurt or killed, before everything she cares about is ripped away from her and her family forever. To solve this mystery - and live to tell about it - Zenn will have to put her new exovet skills to work in ways she never imagined, and in the process learn just how powerful compassion and empathy can be.