- Table View
- List View
In Vitro Models for Stem Cell Therapy: Methods and Protocols (Methods in Molecular Biology #2269)
by Peggy Stock Bruno ChristThis volume looks at in vitro disease models representing the respiratory, hepatobiliary, osteochondral, nervous, dermal, ocular, immune system, and pathological biological processes like tumorigenesis for stem cell research. The chapters in this book cover a range of diseases and application of various stem cells such as adult stem cells and iPS. Chapters also discuss new methods to characterize and manipulate stem cells with the aim to better understand and improve their biological performance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, In Vitro Models for Stem Cell Therapy: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this exciting field
In Vitro Mutagenesis Protocols, 2nd Edition
by Jeff BramanHands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.
In Vitro Mutagenesis Protocols, 3rd Edition
by Jeff BramanIn the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.<P><P> Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.
In Vitro Neurochemical Techniques
by Alan N. Bateson Glen B. Baker Alan A. BoultonThis collection of state-of-the-art techniques encompasses the breadth of neurochemical and molecular neurobiology research, ranging from the isolation of neuronal genes and the study of their expression to the analysis of receptor-ligand interactions, to the characterization of the consequences of receptor activation. The methods include electrophysiological techniques to explore the functional properties of receptors present in the membranes of excitable cells, methods to isolate novel genes central to neurobiological processes, and protocols to perform in situ hybridization histochemistry. Other methods cover measuring changes in gene expression, the rapid identification of gene polymorphisms, and the identification and characterization of second messenger pathways. Together with its companion volumes, In Vivo Neuromethods and Cell Neurobiology Techniques, all three cutting-edge works will prove exceptionally useful to those basic and clinical neuroscientists who want to expand the range of their current research or develop competence in complementary methods.
In Vitro Neuronal Networks: From Culturing Methods to Neuro-Technological Applications (Advances in Neurobiology #22)
by Michela Chiappalone Valentina Pasquale Monica FregaThis book provides a comprehensive overview of the incredible advances achieved in the study of in vitro neuronal networks for use in basic and applied research. These cultures of dissociated neurons offer a perfect trade-off between complex experimental models and theoretical modeling approaches giving new opportunities for experimental design but also providing new challenges in data management and interpretation. Topics include culturing methodologies, neuroengineering techniques, stem cell derived neuronal networks, techniques for measuring network activity, and recent improvements in large-scale data analysis. The book ends with a series of case studies examining potential applications of these technologies.
In Vitro Neurotoxicology: Methods and Protocols
by Lucio G. Costa Marina Guizzetti Gennaro GiordanoIn recent years, the need to develop acceptable alternatives to conventional animal testing for neurotoxicity and developmental neurotoxicity has been increasingly recognized, and much effort is being directed toward the development of alternative models, utilizing mostly mammalian cells in culture but also non-mammalian model systems. In Vitro Neurotoxicology: Methods and Protocols presents a series of cellular, biochemical, and molecular methodological protocols in the area of in vitro neurotoxicology, with an emphasis on mammalian cell culture systems. Opening with a section on methodologies for preparing several cellular systems of variable complexity, amenable for in vitro neurotoxicological studies, the thorough volume continues with coverage of methods to measure cellular death and major mechanisms, methods for assessing mechanisms of nervous system cell toxicity related to impairment of cell signaling, while a final section illustrates additional methods for assessing important nervous system processes such as cell proliferation, neuritogenesis, and synaptogenesis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, In Vitro Neurotoxicology: Methods and Protocols serves researchers with an interest in assessing or characterizing the potential neurotoxicity of environmental contaminants, drugs, or other chemicals.
In Vitro Neurotoxicology: Principles and Challenges
by Evelyn Tiffany-CastiglioniNeurotoxicity assessment with in vitro systems offers not only a means to better understand mechanisms of action of toxicants and decrease reliance on laboratory animals, but also provides a basis for reliable, high throughput screening of new and untested chemicals. In In Vitro Neurotoxicology: Principles and Challenges, well-respected leaders in the field take a fresh look at their own and others' work, critically and comparatively analyzing it across experimental systems and toxicants, and synthesizing essential principles for in vitro neurotoxicity testing. In thematically organized chapters, the authors employ examples comprised of multiple experimental systems and endpoints, and discuss the use of complementary in vivo/in vitro strategies. Neurotoxicants of significance to human health are emphasized, especially those for which metabolism and dose-responses are well studied both in vivo and in vitro: lead, mercury, organophosphorus insecticides, polychlorinated biphenyls and dioxin, ethanol, and endogenous proteins. Several neurotoxicology topics covered provide insight into neurodevelopmental and neurodegenerative diseases, including the concept of astroglia as depots for lead and other metals in the central nervous system, the elucidation of factors involved in the onset of puberty in females, and the exploration of endogenous proteins as neurotoxicants. <P><P> Authoritative and forward-looking, In Vitro Neurotoxicology: Principles and Challenges sets out new concepts and research directions that will facilitate significant improvement in today's methods and systems for in vitro neurotoxicity testing.
In Vitro Percutaneous Absorption: Principles, Fundamentals, and Applications
by Robert L. Bronaugh Howard I. MaibachThis book covers all major areas of interest in the rapidly expanding field of in vitro methods for percutaneous absorption studies. Specific areas discussed include diffusion cell design, receptor fluid, preparation of skin, and temperature. The book covers experimental methodology, as well as the underlying principles and fundamentals that help professionals and students gain an understanding of the basis for currently used methodology.
In Vitro Toxicity Testing Protocols
by Christopher K. Atterwill Sheila O’hareIn Vitro Toxicity Testing Protocols is a collection of proven, state-of-the-art methods that toxicologists can use in carrying out laboratory studies. The book's expert contributors offer a variety of efficient in vitro toxicology techniques that can be applied regularly in your lab, or can serve as the fundamental basis for the establishment of novel techniques in working laboratories. All the procedures are highly reliable. Also included are ex vivo techniques, whose development embraces the twin aims of reduction and refinement of the use of laboratory animals; the editors believe this is an important step toward the ethical treatment of laboratory animals. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, concise, easy-to-follow, step-by-step protocols are provided for each technique, together with extensive notes that cover troubleshooting and offer special tips to ensure the successful completion of your experiments.
In Vitro Toxicology Systems
by Anna Bal-Price Paul JenningsIn Vitro Toxicology Systems brings together important issues and considerations needed in order to develop a workable, reliable, integrated testing strategy for the replacement of animals in toxicity testing regimes. This thorough volume includes sections on in vitro models for systemic organ toxicity, neurotoxicity, sensory organs, immunotoxicity and reproductive toxicity and addresses how stem cells may be used going forward. The book also tackles difficult areas of toxicology such as carcinogenicity and nanotoxicology, with additional chapters dedicated to kinetics, metabolism, and in vitro in vivo extrapolation. The book also addresses biological processes such as stress response pathways and mechanistic biomarkers and how these can be uncovered and measured using high content approaches. Reliable and authoritative, In Vitro Toxicology Systems will be of benefit not only to students, scientists, and regulators working in the field of chemical safety assessment but also to a wider scientific audience. --
In Vitro Transcription and Translation Protocols
by Guido GrandiA highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included.
In Vivo Cellular Imaging Using Fluorescent Proteins
by Robert HoffmanThe discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.
In Vivo Confocal Microscopy in Eye Disease
by Golshan Latifi Scott HauIn Vivo Confocal Microscopy in Eye Disease is a comprehensive new text that covers the latest advances in the field of in vivo confocal imaging. It presents a detailed overview of the basic anatomy of the different part of the cornea, conjunctiva and adnexal structures. It discusses the use of in vivo confocal microscopy in a range of clinical applications including the diagnosis of infective keratitis, corneal dystrophies, cornea nerves, conjunctival diseases and their differentiating features. Numerous confocal images, clinical pictures, other paraclinical images and histopathology slides of different ocular pathologies are examined and presented throughout the text. This book systematically reviews the use of confocal imaging in basic science of ocular tissues to the diagnosis of eye disease assembled in a single volume. It provides an up to date resource with an in depth review of scientific literature relating to both the clinical and research application of in vivo confocal microscopy in eye disease. The book ends with a discussion on non-ocular application of in vivo confocal microscopy and future developments including other emerging imaging technologies. This text will provide an invaluable resource for those who are interested in ocular imaging and it represents a timely resource for all practicing and trainee ophthalmologists
In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs
by Shinichi Ohno Nobuhiko Ohno Nobuo TeradaThis book focuses on actual morphofunctional findings of cells and tissues in living animal organs. Medical and biological scientists need to know the real in vivo morphology and immunolocalization of the molecular components in living animal organs. Recently, the live imaging of cells and tissues of animals with fluorescence-labeled proteins by gene manipulation has become more and more popular in biological fields. Current research, meanwhile, has revealed that immunohistochemical or morphological studies exclusively depend on living animal organs. The cryotechnique is one of the most useful tools for immunohistochemistry and bioimaging of animal organs. This book describes the epoch-making cryotechnique originally developed by the editors. The book also makes the management of living animal morphology more accessible not only for biomedical researchers but also for clinical doctors, providing a valuable resource work on the current perspectives of in vivo morphology.
In Vivo Inhalation Toxicity Screening Methods for Manufactured Nanomaterials (Current Topics in Environmental Health and Preventive Medicine)
by Toru Takebayashi Robert Landsiedel Masashi GamoThis edited volume discusses the short-term inhalation study (STIS) and intratracheal administration, the two major in vivo inhalation-toxicity screening methods, which play an important role in efficient hazard evaluation. It also provides a general overview of the inhalation toxicity of nanomaterials and related issues. For each screening method, it provides up-to-date information on the test procedures, interpretation of the test results, useful applications, and related technologies. In view of the increasing variety of nanomaterials in practical use, the book offers a basis for building a framework for grouping and read-across assessments of nanomaterials. With contributions by academic and industrial experts, In vivo Inhalation Toxicity Screening Methods for Manufactured Nanomaterials is a pragmatic reference resource for readers who are responsible for assessing the safety of nanomaterials in R&D and business, as well as researchers.
In vivo Models for Drug Discovery, Volume 62
by Hugo Kubinyi Michel Hamon Raimund Mannhold José M Vela Gerd Folkers Rafael MaldonadoThis one-stop reference is the first to present the complete picture -- covering all relevant organisms, from single cells to mammals, as well as all major disease areas, including neurological disorders, cancer and infectious diseases. Addressing the needs of the pharmaceutical industry, this unique handbook adopts a broad perspective on the use of animals in the early part of the drug development process, including regulatory rules and limitations, as well as numerous examples from real-life drug development projects. After a general introduction to the topic, the expert contributors from research-driven pharmaceutical companies discuss the basic considerations of using animal models, including ethical issues. The main part of the book systematically surveys the most important disease areas for current drug development, from cardiovascular to endocrine disorders, and from infectious to neurological diseases. For each area, the availability of animal models for target validation, hit finding and lead profiling is reviewed, backed by numerous examples of both successes and failures among the use of animal models. The whole is rounded off with a discussion of perspectives and challenges. Key knowledge for drug researchers in industry as well as academia.
In Vivo Neuromethods
by Alan A. Boulton Glen B. Baker Alan N. BatesonA superb collection of time-tested, key techniques for investigating nervous system function, as well as the actions of drugs on the nervous system. The methods range from authoritative treatment of such well-established techniques as recording electrical activity in the brain, to practical coverage of state-of-the-art methods, including neural transplantation and antisense technology. The techniques described are particularly suitable for studying the mechanisms of action of psychotropic drugs and drugs of abuse, as well as the etiology and pharmacotherapy of neuropsychiatric diseases and neurodegenerative disorders. Several of the methods detailed are highly useful in screening potential drugs for the treatment of depression, anxiety disorders, schizophrenia, and Parkinson's and Alzheimer's disease.
In Vivo Neuropharmacology and Neurophysiology
by Athineos PhilippuThis volume presents classical approaches to in vivo neuropharmacology and neurophysiology, such as c-fos, electrochemistry, microdialysis microstimulation, and push-up superfusion. It also explores exciting new methods for behavioral analysis, and techniques based on optogenetics and non-invasive magnetic resonance imaging. The chapters of this book cover topics such as principles of stereotaxy, pharmaco-based fMRI and neurophysiology in humans and non-human primates, electrical nerve stimulation and central microstimulation, involvement of neurotransmitters in mnemonic processes, the impact of cannabinoids on motor activity, as well as the involvement of nitric oxide in neurotoxicity produced by psychostimulant drugs. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Cutting-edge and practical, In Vivo Neuropharmacology and Neurophysiology is a valuable resource for experienced and less experienced investigators of brain function and brain disorders.
In vivo NMR Imaging
by Leif Schröder Cornelius FaberNuclear magnetic resonance imaging represents a technique that is indispensable in every day biomedical diagnostics. Thanks to the numerous ways to manipulate and detect an NMR signal, it is possible to obtain a variety of information with excellent spatial and temporal resolution. Today's MRI techniques go far beyond the illustration of pure anatomical structures and include the revealing of processes down to the molecular level. The number of small animal imaging centers relying on MRI as a key method for preclinical research to understand diseases and to test for novel treatments is growing rapidly. In Vivo NMR Imaging: Methods and Protocols is written as an experimental laboratory text to provide a descriptive approach of the various applications of magnetic resonance imaging and its underlying principles. Starting from a compact introduction of basic NMR physics and image encoding techniques suitable for a broad audience in the life sciences, the concept focuses on addressing the many ways of generating contrast in MR images. The authors cover an interdisciplinary range of problems to be addressed by this non-invasive modality, including study protocols for addressing morphological, physiological, functional, and biochemical aspects of various tissues in living organisms. Information about practical aspects of designing experimental studies that follow the special conditions for micro imaging setups are also provided. Written in the successful Methods in Molecular BiologyTM series format, In Vivo NMR Imaging: Methods and Protocols aims to be an experimental compendium of modern in vivo MR imaging with special focus on recent developments in molecular imaging and new protocols for imaging metabolism and molecular markers.
In Vivo Reprogramming in Regenerative Medicine
by Açelya YilmazerThis new volume reviews current progress on different approaches of in vivo reprogramming technology. Leaders in the field discuss how in vivo cell lineage reprogramming can be used for tissue repair and regeneration in different organs, including brain, spinal cord, pancreas, liver and heart. Recent studies on in vivo cell reprogramming towards pluripotency are reviewed; examples are given to show its potential in regenerative medicine. In each chapter, the regenerative potential of different in vivo reprogramming approaches is discussed in detail. More specifically, how different tissue failures or damages can be treated with this technology is explained. Examples from various animal models are given and the regenerative potential of in vivo reprogramming is compared to that of cell transplantation studies. The last chapter discusses current challenges of these preclinical studies and gives suggestions in order to improve the current strategies. Future directions are indicated for the transition of in vivo reprogramming technology to clinical settings. This is among the first books in the literature which specifically focuses on the in vivo reprogramming technology in regenerative medicine and these chapters collectively cover one of the most important and exciting topics of regenerative medicine.
In Vivo Self-Assembly Nanotechnology for Biomedical Applications
by Hao Wang Li-Li LiThis book reviews and discusses the development of self-assembled nanomaterials applied in biomedical fields. Based on self-assembled nanomaterial constructions, it highlights the mechanisms of the stimuli-response-induced assembly/disassembly and transformation. Moreover, it examines healthcare-related diseases, the applications of nanomaterials and therapy/detection strategies, providing readers with both a deeper understanding of the subject and inspirations for future research. The book is primarily intended for researchers and graduate students in the fields of material sciences and chemistry who wish to learn about the principles, methods, mechanisms and biomedical applications of self-assembled nanomaterials.
Inborn Metabolic Diseases
by Jean-Marie Saudubray Matthias R. Baumgartner John WalterThis work is recognised as the standard textbook for professionals involved in the diagnosis and management of inborn errors of metabolism (IEM) and an essential resource in this multidisciplinary field. For the 6th edition all 43 chapters have been newly written or revised by authors with particular expertise in their subject areas. Contents: A clinical and biochemical approach to the recognition and diagnosis of IEM with algorithms to symptoms, signs, and syndromes in patients of all ages; Emergency treatments; Medications - Separate comprehensive sections on IEM of: Carbohydrates; Mitochondrial Energy; Amino and organic acids; Vitamin-responsive defects; Neurotransmitter and Small peptides, Lipid and Bile Acids; Nucleic Acid and Heme; Organelles - Disorders affecting the synthesis and remodelling of complex lipids and fatty acid homeostasis are now included.
Inborn Metabolic Diseases: Diagnosis and Treatment
by Jean-Marie Saudubray Matthias R. Baumgartner John H. Walter Ángeles García-CazorlaThis 7th edition is a milestone in the series of Inborn Metabolic Diseases (IMD), recognised as the standard textbook for professionals involved in the diagnosis and management of IMD.Within the last 5 years a Copernican revolution in our understanding of IMD has changed the definition, concepts, paradigms, and classification. This new edition now extends the concept of IMD to include those disturbances in molecular machinery diagnosed by molecular techniques but currently without measurable metabolic markers.The book presents a clinical and biochemical approach to the diagnosis and management of IEM with many diagnostic algorithms for patients of all ages and with a particular focus on neurological presentations. It includes separate, comprehensive sections on IEM classified in 3 major pathophysiological categories: disorders of energy metabolism, both mitochondrial and non-mitochondrial; small molecule disorders, mostly diagnosed with metabolic markers; and complex molecules disorders, mostly diagnosed with molecular techniques.Two new chapters were added, describing around 600 disorders of nucleic acid metabolism, tRNA metabolism, ribosomal biogenesis, and cellular trafficking.
Incarnate: A Novel
by Josh StolbergAn ambitious and sharp-witted clinical psychiatrist turns detective when one of her patients comes under investigation for a series of brutal murders—is she a psychopath or a victim herself?Brilliant psychiatric resident Dr. Kim Patterson has one major flaw: she is too committed to her patients. Her willingness to break the rules and use unconventional methods to help her patients heal has already gotten her thrown out of several hospitals. And when Scarlett Hascall is brought into the psychiatric ward, Kim can’t resist getting involved, even if it means breaking protocol—because Kim can see what the other doctors cannot. Scarlett is suffering from a rare condition: Dissociative Identity Disorder, otherwise known as Multiple Personalities. But even Kim is at a loss when she realizes that some of Scarlett’s alternate identities are claiming to be people who have gone missing from their town. And when Scarlett’s alter identity “Izzi” knows way too much about the whereabouts of the missing Isabel Wilcox, the police begin to suspect that Scarlett may have been involved with Isabel’s disappearance. Only Kim defends Scarlett, certain of the girl’s innocence. But her new theory is a radical one, one she can hardly believe herself: What if Kim is channeling the troubled souls of these victims? With the help of local police officer Zack Trainor, Kim digs further into Isabel’s case, trying to find proof that Scarlett wasn’t involved. The more they discover, the more it becomes apparent that something strange and frightening is going on with Scarlett. Is it possible she is truly harboring lost souls? Or is Scarlett playing a twisted game with her doctors and the police?
The Incense Bible: Plant Scents That Transcend World Culture, Medicine, and Spirituality
by Dennis J Mckenna Kerry HughesMake a spiritual connection to nature with real, raw, natural incenseThe Incense Bible is a comprehensive guide to the spiritual meaning of real, raw, natural incense and how to use it in prayer, meditation, or simply in creating a home environment to "reconnect" with divinity and nature. This unique book examines the spiritual and ritual uses of "pure" incense (not sticks, cones, or synthetic mixtures), explores our attraction to it, and explains how we can use it at home to increase wellness. Filled with easy-to-use references and easy-to-understand technical information, the book also looks at the use of incense in health and medicine, a history of its use in a variety of countries and cultures, and various types of raw incense, including eucalyptus, sandalwood, sweet grass, agarwood, frankincense, and myrrh.The Incense Bible examines aspects of spirituality and religion, health and medicine, botanical medicines, ethnobotany, and history of real incense-not the low-quality, processed, synthetic fragrances most people think of as "incense." This enlightening and entertaining book, written in everyday language and filled with photgraphs, examines why incense appeals to our sense of smell, of adventure, and of spiritual and physical well-being-and has for centuries. The book looks at the use of incense for purifications and cleansings, creative inspiration, meditation, worship and prayer, for inducing dreams and sleep, to improve learning and problem solving, and as a perfume for clothes, hair, and body.Topics examined in The Incense Bible include: types of raw incense, including balsam, cedar, mugwort and moxa, and white sage safety how the sense of smell works the link between taste and scent scent and memory allergies and sensitivities oils and flower essences for healing how to make incense incense materials and symbols the use of incense in Egyptian, Greek, and Roman times, in Buddhism and Shamanism, in Israel, in the Catholic Church, in Native American sweat lodges, and in the African rainforest and much more!The Incense Bible is an essential resource for anyone interested in health and natural medicine, botanical medicines, and ethnobotany, and for anyone seeking to express their spirituality by reconnecting to nature