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Peptide-Based Drug Design
by Laszlo OtvosDue to their high specificity and low toxicity profile, peptides have once again become central to the development of new drugs. In Peptide-Based Drug Design: Methods and Protocols, expert researchers provide a handbook which offers a selection of research and production tools suitable for transforming a promising protein fragment or stand-alone native peptide into a pharmaceutically acceptable composition. The volume delves into contemporary, cutting-edge subjects such as hit isolation and target validation, computer-aided design, sequence modifications to satisfy pharmacologists, in vivo stability and imaging, and the actual production of difficult sequences. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include readily reproducible, step-by-step laboratory protocols, lists of materials, and the Notes section, which highlights tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Peptide-Based Drug Design: Methods and Protocols shows its subject to be an independent science on the rise, and provides scientists with a clear, concise guide for continuing this vital research.
Peptide Chemistry and Drug Design
by Ben M. DunnThis book focuses on peptides as drugs, a growing area of pharmaceutical research and development. It helps readers solve problems of discovering, developing, producing, and delivering peptide-based drugs. * Identifies promising new areas in peptide drug discovery * Includes chapters on discovery from natural sources, metabolic modification, and drug delivery * Overviews separation methods and techniques for analysis, bond formation, and purification * Offers readers both a professional reference and a text or resource for graduate-level students
Peptide Drug Discovery and Development: Translational Research in Academia and Industry
by Castanho Nuno C. SantosFilling a real knowledge gap, this handbook and ready reference is both modern and forward-looking in its emphasis on the "bench to bedside" translational approach to drug development. Clearly structured into three major parts, the book stakes out the boundaries of peptide drug development in the preclinical as well as clinical stages. The first part provides a general background and focuses on the characteristic strengths and weaknesses of peptide drugs. The second section contains five cases studies of peptides from diverse therapeutic fields, and the lessons to be learned from them, while the final part looks at new targets and opportunities, discussing several drug targets and diseases for which peptide drugs are currently being developed.
Peptide Libraries
by Ratmir DerdaThis volume provides an overview of modern and emerging methods for production, analysis, and utility of peptide libraries. Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these libraries, de novo discovery of reactions, hybrid organic-inorganic materials and, emerging tools for the analysis of these libraries by method of genetic selection and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Peptide Libraries: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.
Peptide Microarrays
by Marina Cretich Marcella ChiariDue to their versatility, along with the diminishing costs of library synthesis and the growth of commercial support, peptide microarrays will likely expand beyond being just a research tool into an adaptable and powerful platform to be harnessed for wider drug discovery and point-of-care applications. In Peptide Microarrays: Methods and Protocols, experts in the field provide a cutting-edge view of peptide array technology, its applications, and technical issues. After examining how peptides can characterize proteins and clarify, at the amino acid level, the molecular recognition events in which they are involved, the volume goes on to cover topics such as the production and use of peptide arrays for enzyme and binding motifs characterization, epitope mapping, and diagnostics, the newest technological advancements, as well as software and web tools for the design of peptide arrays and for the analysis of output data. As a volume in the highly successful Methods in Molecular BiologyTM series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Peptide Microarrays: Methods and Protocols promises to serve scientists with its unique insights and novel solutions in peptide array technology in order to advance this vital field.
Peptide Microarrays: Methods and Protocols (Methods in Molecular Biology #2578)
by Marina Cretich Alessandro GoriThis detailed volume provides an updated overview of current uses of peptide microarray technology, showcasing consolidated applications while highlighting some of the most intriguing novelties and emerging fields of use. The methodologies within this collection are of considerable value for both advanced users and new-comers in the peptide microarray arena, as the renowned contributors describe full coverage of the aspects related to their workflows, from microchip manufacturing to advanced analytical applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Peptide Microarrays: Methods and Protocols, Third Edition aims to make the use of peptide microarrays more and more accessible while stimulating further developments to sustain exciting discoveries in the biochemistry and medicine realms.
Peptide Research Protocols
by Anthony P. Davenport Janet J. MaguireA panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of the endothelin system from its synthesis and metabolism, to its function in health and disease. Theses methods use state-of-the-art molecular techniques to quantify the expression of mRNA for both endothelin receptors and the endothelin converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function.
Peptide Synthesis: Methods and Protocols (Methods in Molecular Biology #2103)
by Waleed M. Hussein Mariusz Skwarczynski Istvan TothThis book provides a variety of procedures for synthetically producing peptides and their derivatives, ensuring the kind of precision that is of paramount importance for successful synthesis. Numerous techniques relevant to drugs and vaccines are explored, such as conjugation and condensation methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Peptide Synthesis: Methods and Protocols serves as an essential guide to the many crucial processes that will allow researchers to efficiently prepare, purify, characterize, and use peptides for chemical, biochemical, and biological studies.
Peptide Therapeutics: Fundamentals of Design, Development, and Delivery (AAPS Advances in the Pharmaceutical Sciences Series #47)
by Seetharama D. JoisThis book explains how peptide-based drug design works, what steps are needed to develop a peptide-based therapeutic, and challenges in synthesis as well as regulatory issues. It covers the design concept of peptide therapeutics from fundamental principles using structural biology and computational approaches. The chapters are arranged in a linear fashion. A fresh graduate or a scientist who works on small molecules can use this to follow the design and development of peptide therapeutics to use as understanding the basic concepts. Each chapter is written by experts from academia as well as industry. Rather than covering extensive literature, the book provides concepts of design, synthesis, delivery, as well as regulatory affairs and manufacturing of peptides in a systematic way with examples in each case. The book can be used as a reference for a pharmaceutical or biomedical scientist or graduate student who wants to pursue their career in peptide therapeutics. Some chapters will be written as a combination of basic principles and protocol so that scientists can adopt these methods to their research work. The examples provided can be used to perform peptide formulation considerations for the designed peptides. The book has nine chapters, and each chapter can be read as an independent unit on a particular concept.
Peptides (Neuromethods #6)
by Glen B. Baker Q. J. Pittman Alan A. BoultonThis timely new volume in Bouton and Bader's highly practical Neuromethods series presents state-of-the-art techniques for neuropeptide research. Neuromethods * 6 * Peptides highlights the vast array of novel techniques that are essential for identifying neural peptides and explaining their roles, which have become a significant focus of attention in neuropharmacology today. In addition to providing a detailed methodological formula for each technique, the authors - all renowned experts in their particular areas - also demonstrate how their powerful new methodologies contribute to our understanding of neuropeptide function. Chapters explore: * posttranslational processing, * radioimmunoassay development, * in vitro peptide release, * peptide analogs, * molluscan models, * anitbody production, * immunohistochemical localization, * peptide release in vivo, * postsynaptic sequences, * electrophysiological techniques
Peptides and Protein Phosphorylation
by B.E. KempThis comprehensive volume focuses on the ways in which synthetic peptides have been exploited in order to expand our understanding of the molecular mechanisms involved in protein phosphorylation. It recognizes that virtually all physiological processes are regulated by protein phosphorylation. It discusses the use of synthetic peptides in studying the catalytic mechanism and regulation of protein kinases. It also includes the chemical synthesis of phosphorylated peptides and preparation of specific antisera. This incredible work has lead to the development of a new generation of peptide inhibitors with potencies of greater magnitude than those previously known. Everyone involved with biochemistry and molecular biology will find this one-of-a-kind resource fascinating and filled with useful information.
Peptides for Youth
by William D. Lubell Susan Valle Emanuel EscherThe American Peptide Society (APS) provides a forum for advancing and promoting knowledge of the chemistry and biology of peptides. The approximately one thousand members of the Society come from North America and from more than thirty other countries throughout the world. Establishment of the APS was a result of the rapid worldwide growth that has occurred in peptide-related research, and of the increasing interaction of peptide scientists with virtually all fields of science. Peptides for Youth: The Proceedings of the the 20th American Peptide Symposium will highlight many of the recent developments in peptide science, with a particular emphasis on how these advances are being applied to basic problems in biology and medicine. The 20th American Peptide Symposium will take place June 26 - 30, 2007 in Montreal, Canada.
Peptides in Energy Balance and Obesity
by Gema FrühbeckObesity is one of the most relevant public health concerns today and it is now evident that body weight control is achieved through highly integrated physiological interactions like nutrient selection as well as being influenced by genetic and environmental factors. Moreover, energy balance regulation is a complex process aimed at maintaining constant energy stores. Presenting a detailed and comprehensive account of the roles of specific peptides in energy balance, food intake control and co-morbidities, this review provides a better understanding of the patho-physiology of energy balance and obesity.
Per Oral Endoscopic Myotomy (POEM)
by Kevin M. ReavisThis text is designed to present a comprehensive and state-of the-art approach to the current and future status of POEM. Sections address preliminary issues faced by surgeons and gastroenterologists who are interested in implementing this procedure into their treatment armamentarium. These areas include the evolution of the technique, training and credentialing, as well as tools and platforms commonly used for POEM. Subsequent chapters focus on specific disease processes, and future applications of the technique. Written by experts in POEM, Per Oral Endoscopic Myotomy (POEM) addresses patient selection, pre-operative considerations, technique, and avoiding complications. A brief editorial review of the existing literature addressing the particular topic follows in each section. The text concludes with chapters on emerging and future applications. Extensive endoscopic images and illustrations make this an interactive text for General Surgeons, Surgical Endoscopists, Gastroenterologists, and associated sub-specialists.
Perceived Health and Adaptation in Chronic Disease
by Francis Guillemin Alain Leplege Serge Briancon Elisabeth Spitz Joel CosteChronic diseases have become predominant in Western societies and in many developing countries. They affect quality of life and daily activities and require regular medical care. This unique monograph will bring readers up to date with chronic disease research, with a focus on health-related quality of life and patient perception of the impact of the diseases and health intervention, as well as psychological adaptation to the disease. It considers the application of concepts and measures in medical and psychological clinical practice and in public health policies. Informed by theory, philosophy, history and empirical research, chapters will indicate how readers might advance their own thinking, learning, practice and research. The book is intended to be provocative and challenging to enhance discussion about theory as a key component of research and practice. Perceived Health and Adaptation in Chronic Disease will be of interest to researchers and academics alike. It boasts a wide range of contributions from leading international specialists from Australia, Canada, Denmark, France, Germany, the Netherlands, Spain, Sweden, the UK and the USA. This has also allowed the book to provide readers with a multidisciplinary approach.
Perceptie van spraak: Deel 8 - Handboek Stem-, Spraak- en Taalpathologie
by H.F.M. Peters R. Bastiaanse J. Van Borsel P.H.O. Dejonckere K. Jansonius-Schultheiss Sj. Van der Meulen B.J.E. MondelaersHet Handboek Stem-Spraak-Taalpathologie verscheen tussen 1997 en 2007 gefaseerd in losse afleveringen. Daarin werd alle kennis op het gebied van de stem-, spraak- en taalpathologie vanuit verschillende disciplines samengebracht. Het Handboek is bestemd voor iedereen die klinisch-praktisch of meer theoretisch is geïnteresseerd, of vanuit een ander vakgebied hiermee in aanraking komt. Voor logopedisten, artsen, linguïsten, spraak- en taalpathologen, audiologen, pedagogen en psychologen in Nederland en België is het Handboek een onmisbare vraagbaak.Deel 8 is gericht op de auditieve waarneming van spraak. Naast de ontwikkeling van het gehoororgaan, komt ook de relatie tussen spraakperceptie en spraakproductie aan bod.
Perception-Action Cycle
by John G. Taylor Vassilis Cutsuridis Amir HussainThe perception-action cycle is the circular flow of information that takes place between the organism and its environment in the course of a sensory-guided sequence of behaviour towards a goal. Each action causes changes in the environment that are analyzed bottom-up through the perceptual hierarchy and lead to the processing of further action, top-down through the executive hierarchy, toward motor effectors. These actions cause new changes that are analyzed and lead to new action, and so the cycle continues. The Perception-action cycle: Models, architectures and hardware book provides focused and easily accessible reviews of various aspects of the perception-action cycle. It is an unparalleled resource of information that will be an invaluable companion to anyone in constructing and developing models, algorithms and hardware implementations of autonomous machines empowered with cognitive capabilities. The book is divided into three main parts. In the first part, leading computational neuroscientists present brain-inspired models of perception, attention, cognitive control, decision making, conflict resolution and monitoring, knowledge representation and reasoning, learning and memory, planning and action, and consciousness grounded on experimental data. In the second part, architectures, algorithms, and systems with cognitive capabilities and minimal guidance from the brain, are discussed. These architectures, algorithms, and systems are inspired from the areas of cognitive science, computer vision, robotics, information theory, machine learning, computer agents and artificial intelligence. In the third part, the analysis, design and implementation of hardware systems with robust cognitive abilities from the areas of mechatronics, sensing technology, sensor fusion, smart sensor networks, control rules, controllability, stability, model/knowledge representation, and reasoning are discussed.
Perceptions of Health and Illness: Current Research And Applications
by Keith J. Petrie John A. WeinmanFirst Published in 1997. The study of how individuals perceive and make sense of health and illness is a new and rapidly developing area in health psychology. The field has seen important recent theoretical developments and applications to a wide range of health threats and illnesses. The first section of this book examines the current theoretical and measurement issues in the field and includes issues related to illness perceptions across the lifespan, disability, and the assessment of illness representations in chronic illness. The second section addresses the role of illness perceptions in health screening and prevention and includes work on perceptions of genetic disease, cancer screening, and how individuals process health risk information. The third section is concerned with the application of the illness perceptions approach to patients with chronic illness and those undergoing treatment. Illnesses examined using this approach include chronic fatigue syndrome, breast cancer, diabetes, and myocardial infarction.
Perceptual Organization: An Integrated Multisensory Approach
by Stephen HandelThis textbook goes beyond introductory sensory perception by incorporating supplementary electronic materials to demonstrate the parallels between both hearing and seeing. Each chapter intermixes seeing and hearing processes so that students can easily understand that perceptual organization is the same across different kinds of sensations and modalities. Figures illustrating visual organization are paired with sound files demonstrating the analogous auditory organization. While most books on sensation and/or perception treat the senses individually there is growing awareness of just how important multisensory integration is to understanding the connection between sensory perception and cognition.
Percutaneous Absorption: Drugs, Cosmetics, Mechanisms, Methods (Drugs and the Pharmaceutical Sciences #155)
by Nina DragićevićUpdating and expanding the scope of topics covered in the previous edition, Percutaneous Absorption: Drugs, Cosmetics, Mechanisms, Methods, Fifth Edition supplies new chapters on topics currently impacting the field including cutaneous metabolism, skin contamination, exposure to protein allergens, in vitro absorption methodology and the percutaneous absorption of chemical mixtures. Complete with studies on the role of the skin as a key portal of entry for chemicals into the body, this book serves as a detailed reference source for recent advances in the field, as well as an experimental guide for laboratory personnel. Key Features: Details in vivo and in vitro methods for measuring absorption, dermal decontamination, mechanisms of transdermal delivery, and the relationship of transepidermal water loss to percutaneous absorption Considers a range of mathematical models, the safety evaluation of cosmetic ingredients, the absorption of hair dyes, nanoparticles for drug delivery, and other novel methods of drug delivery Discusses topics including skin metabolism, the skin reservoir, and the effects of desquamation on absorption
Percutaneous Absorption of UV Filters Contained in Sunscreen Cosmetic Products
by Zacarías León GonzálezZacarías León's thesis describes the development and validation of analytical methods to estimate the processes set in motion by percutaneous absorption of UV filters in sunscreen cosmetic products. León describes these methods in both in vitro and non-invasive in vivo methodologies. Currently dermatologists recommend the use of sunscreen products not only under conditions of extreme exposure to the sun but also in daily situations. However the chemical compounds in these products contain may lead to undesired processes and cause induced toxicity, estrogenic effects and endocrine activity. León establishes methods to investigate these effects and provides valuable information on the undesired side effects associated with the use of UV filters found in sunscreen products. The work in this thesis has led to a number of publications in renowned analytical chemistry journals.
Percutaneous and Minimally Invasive Foot Surgery
by Cyrille Cazeau Yves StiglitzThis book on percutaneous and minimally invasive foot surgery comprehensively covers one of the most innovative topics in orthopedic surgery, discussing decision-making based on foot biomechanics and presenting step-by-step descriptions of each surgical procedure. The chapters are divided into four sections, the first of which lays the groundwork for further reading by providing a comprehensive overview of the biomechanical approach. The second part then guides readers through making appropriate choices in order to ensure stable corrections and allow immediate full weight-bearing and fast rehabilitation without compromising patient outcomes. In the third part, leading surgeons in the field describe each procedure step-by-step with the help of operative pictures, while the last part addresses technical postoperative issues, such as postoperative dressing and cast management, as well as complications and how to reduce them. All chapters are richly illustrated and clearly highlight the key points for successful surgery and minimized risks. Unique in its focus on percutaneous and minimally invasive foot surgery, this book offers an invaluable resource for postgraduate students, residents, orthopedists wanting to specialize in foot surgery, as well as practicing orthopedic surgeons and podiatrists.
Percutaneous and Non-fluoroscopical (PAN) Procedure for Structural Heart Disease
by Horst Sievert Ziyad M. Hijazi Xiangbin PanThis book provides an extensive discussion of echocardiography-guided percutaneous interventional techniques for clinical practitioners, including cardiologists, interventional physicians, surgeons and specialist training candidates. It allows readers to gain a thorough understanding of interpreting echocardiography-guided procedures, which not only protect patients from pain and other complications associated with conventional surgery, but also obviate the use of fluoroscopy or contrast agents and so avoid radiation damage and the risks of allergy or renal function impairment. Further, these procedures could help to save patients in areas lacking medical resources. The book first demonstrates the substitution of transesophageal for transthoracic echocardiography to avoid intubation under general anesthesia, and then presents key technical and practical aspects of echo-guided percutaneous interventions for structural heart diseases, such as ASD, VSD, PDA, PS, MS, AS and CoA. Lastly, it explores the lessons learned in echocardiography, anesthesia and postprocedural management of echo-guided percutaneous interventions.
Percutaneous Collagen Induction With Microneedling: A Step-by-Step Clinical Guide
by Emerson Lima Mariana LimaPercutaneous Collagen Induction with Microneedling is a minimally invasive technique widely used to treat numerous dermatologic conditions such as facial and body scars, melasma, wrinkles, skin laxity, stretch marks, alopecies, vitiligo and scleroderma. Microneedling can also be used to optimize transdermal drug delivery for many substances. This technique uses modern microneedling devices containing multiple fine needles, typically 0.5 to 2.5 mm in length, which are mounted on a barrel and rolled onto the skin to create numerous perforations into the stratum corneum and the papillary dermis. These micro-wounds initiate the release of growth factors, triggering collagen and elastin formation, which results in dermal remodeling and skin resurfacing. This book provides a step-by-step approach to microneedling, based on the authors’ more than ten years of experience with the technique, during which they have treated more than 3,000 patients in Brazil for numerous dermatologic conditions. Richly illustrated throughout, it includes over 400 illustrations.
Percutaneous Coronary Intervention for Chronic Total Occlusion: The Hybrid Approach
by Stéphane RinfretThe second edition of this essential text provides readers with a detailed guide to performing various percutaneous coronary intervention (PCI) techniques for treating coronary chronic total occlusion (CTO). PCI continues to be an effective procedure to help patients with this pathology, with high success and low complications rates. Chapters feature a step-by-step approach to relevant techniques and describe their potential pitfalls, enabling the reader to develop a thorough understanding of how to perform those procedures successfully. Details of the latest methods for angiography analysis and the management of ostial CTOs, plus heavily revised chapters on topics such as contemporary device-based antegrade dissection and the retrograde approach through septal and non-septal collateral channels ensure that this Work remains the most up-to-date reference on the subject. Percutaneous Intervention for Coronary Chronic Total Occlusion: The Hybrid Approach represents a vital reference to assist practicing and trainee interventional cardiologist in learning these techniques. Various examples are provided, with a vast selection of still images and angiographic video loops to enable the reader become confident in applying these methodologies into their day-to day clinical practice.