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T Cell Protocols
by Kelly P. KearseKelly P. Kearse and a panel of laboratory experts describe the step-by-step execution of a wide variety of basic and specialized techniques for the study of T cell developmental biology and of their subsequent activation. Readily reproducible, the techniques are explained concisely, but in such detail that they are suitable for both the novice and experienced investigator. Coverage includes treatment of both fundamental and novel experimental protocols for the analysis of the role T cells play in immune function and in the prevention and cure of human diseases. Practical and timely, T Cell Protocols: Development and Activation offers basic scientists and clinical investigators a valuable compendium of key protocols that will improve skills with existing methods, as well as afford rapid mastery of powerful new techniques.
T Cell Protocols
by Gennaro De LiberoWith a wide variety of investigative approaches, T cell immunology is a vital and open field of study. In T Cell Protocols, Second Edition, an international panel of experts contribute fully updated classic protocols as well as newly established novel techniques for the study of T lymphocyte biology. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters in this volume provide brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections which collect expert tips on troubleshooting and avoiding known pitfalls. Up-to-date and easy to use, T Cell Protocols, Second Edition is an ideal guide for young investigators new to the complex field of immunology as well as a valuable, concise resource for experienced scientists searching for clear, efficacious descriptions of novel methods.
T-Cell Receptor Signaling: Methods and Protocols (Methods in Molecular Biology #2111)
by Chaohong LiuThis volume provides current and new advanced methods and protocols to study T cells. Chapters guide readers through T cell diversity using mass cytometry, analyzing T cells from single cell level, CRISPR/Cas9 techniques to study the T cell activation, techniques to study subsets of Tcell’s, procedures to study artificial antigen presentosomes for T cell activation, techniques to study the T cell development, two-photon microscopy, and MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Cell Receptor Signaling: Methods and Protocols aims to provide a wide range of approaches and be an invaluable resource for present and future generations of T cell researchers.
T-Cell Repertoire Characterization (Methods in Molecular Biology #2574)
by Huang Huang Mark M. DavisThis volume provides a comprehensive compilation of protocols in T cell repertoire analysis, from the leading experts in the field, representing both well-established methods and cutting-edge advances. Chapters broadly cover the emerging new T cell subsets, sequencing technologies for capturing TCR repertoire, and computational tools for analyzing an ever-growing TCR repertoire, with a particular focus on how to link the sequence with TCR antigen specificity. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Cell Repertoire Characterization aims to be a useful practical guide to researches to help further their study in this field.
T-Cell Trafficking
by Federica M. Marelli-Berg Sussan NoursharghIn the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the development of novel and cutting-edge techniques, particularly in the field of real-time imaging and genetic manipulation, have led to an increased understanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions in vitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responses in experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories.
T-Cell Trafficking
by George Edward Rainger Helen M. McgettrickThis second edition provides updated and new chapters on T-Cell trafficking. In addition to detailed experimental procedures, the interested reader will find informative introductory chapters on the relevance of T-Cell trafficking in thymic population and maturation, traffic through secondary lymphoid organs during 'physiological' resolving inflammation and during immune responses, as well as T-Cell trafficking in chronic inflammatory diseases. Importantly, chapters cover methods from in silico modeling of cellular interactions, in vitro adhesion assays, through ex vivo functional assays to integrated intravital modeling of T-Cell trafficking through organs. Written in the highly successful Methods in Molecular Biology series format, each methods chapter includes a short introduction to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, T-Cell Trafficking: Methods and Protocols, Second Edition aims to be an essential point of reference for those new to the field of T-Cell trafficking, or to those looking to expand their technical capabilities.
T. cruzi Infection: Methods and Protocols (Methods in Molecular Biology #1955)
by Karina Andrea Gómez Carlos Andrés BuscagliaThis volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput ‘-omics’ technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information.
T follicular Helper Cells
by Marion Espéli Michelle LintermanThis volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward.
T-Follicular Helper Cells: Methods and Protocols (Methods in Molecular Biology #2380)
by Luis GracaThe volume provides a comprehensive overview of methods that have been developed for the study of Tfh and Tfr biology in mice, and in different human diseases. The chapters detail methods to investigate the function of Tfh cells in vivo and in vitro, including the use of mathematical models to study GC dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Follicular Helper Cells: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.
T Helper Cell Differentiation and Their Function
by Bing SunThis book will focus on the differentiation and regulation of subsets of CD4+ T cells. It will also cover other aspects of research on these cells, which has made great advances in recent years, such as subsets' plasticity and their role in healthy and disease conditions. The book provides researchers and graduate students with a cutting-edge and comprehensive overview of essential research on CD4+ T cells.
T-Helper Cells: Methods and Protocols (Methods in Molecular Biology #2285)
by Francesco Annunziato Laura Maggi Alessio MazzoniThe aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T-cell manipulation and, last but not least, “omic” approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T- Helper Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
T-Helper Cells
by Ari Waisman Burkhard BecherT-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.
The T.I.G.E.R. Protocol: An Integrative 5-Step Programme to Treat and Heal Your Autoimmunity
by Dr Akil PalanisamyIncrease your resilience to autoimmune diseases by following Dr Akil's 5-step plan for a balanced and healthy diet.Auto-immune disorders like Graves Disease, Hashimoto's Thyroiditis, Lupus, Coeliac and even Long-Covid, affect an estimated 300 million people worldwide. These disorders can be frustrating as they are difficult to diagnose, painful and often invisible. Now, with this simple yet powerful 5-step plan devised by Dr Akil Palanisamy, readers can learn to treat, tame and potentially reverse painful autoimmune conditions.In The T.I.G.E.R. Protocol, Dr Akil Palanisamy uses his training in Western medicine and complementary therapies to create an integrative approach to treat autoimmune diseases. Addressing five key elements - Toxins, Infections, Gut health, Eating right and Rest and balance - this approach has proved remarkably effective for both patients with full-blown disease and those who had been told they were on the path to autoimmunity.The foundation of this approach is the use of specific healing foods. Diet can be a powerful tool, but autoimmune diets that are typically recommended are often restrictive. Dr Akil's protocol expands the diet to be less draconian while maintaining clinical efficacy, potentially reversing the effects of years of suffering from disease and taking a person off the path to suffering in years to come.The protocol is split into easy-to-follow Phase 1 and Phase 2 diets, allowing you to eliminate the necessary food sensitivities in the short term and then reintroduce foods to achieve a more diverse and balanced diet. It also includes Dr Akil's top 10 healing foods for the microbiome - including mushrooms, artichokes and dark chocolate.(P) 2023 Hachette Audio
The T.I.G.E.R. Protocol: An Integrative 5-Step Programme to Treat and Heal Your Autoimmunity
by Dr Akil PalanisamyAuto-immune disorders like Graves Disease, Hashimoto's Thyroiditis, Lupus, Coeliac and even Long-Covid, affect an estimated 300 million people worldwide. These disorders can be frustrating as they are difficult to diagnose, painful and often invisible. Now, with this simple yet powerful 5-step plan devised by Dr Akil Palanisamy, readers can learn to treat, tame and potentially reverse painful autoimmune conditions.In The T.I.G.E.R. Protocol, Dr Akil Palanisamy uses his training in Western medicine and complementary therapies to create an integrative approach to treat autoimmune diseases. Addressing five key elements - Toxins, Infections, Gut health, Eating right and Rest and balance - this approach has proved remarkably effective for both patients with full-blown disease and those who had been told they were on the path to autoimmunity.The foundation of this approach is the use of specific healing foods. Diet can be a powerful tool, but autoimmune diets that are typically recommended are often restrictive. Dr Akil's protocol expands the diet to be less draconian while maintaining clinical efficacy, potentially reversing the effects of years of suffering from disease and taking a person off the path to suffering in years to come.The protocol is split into easy-to-follow Phase 1 and Phase 2 diets, allowing you to eliminate the necessary food sensitivities in the short term and then reintroduce foods to achieve a more diverse and balanced diet. It also includes Dr Akil's top 10 healing foods for the microbiome - including mushrooms, artichokes and dark chocolate.
T Lymphocytes as Tools in Diagnostics and Immunotoxicology
by Stefan F. MartinThis book summarizes the state-of-the art in the development of T cell-based in vitro assays, which offer useful tools for hazard identification, risk assessment and improvement of diagnostics. It will be of interest to scientists, the chemical and pharmaceutical industry, and regulators involved in the replacement of animal testing methods. The identification of hazardous chemicals and drugs is essential to ensuring human health. The ban on animal testing for the cosmetics industry since 2009 and international efforts to reduce and replace animal testing in research and immunotoxicology call for alternative in vitro methods. The most specific immune response to chemicals and drugs that cause allergic contact dermatitis, respiratory disease and adverse drug reactions is the highly antigen-specific T lymphocyte response. Therefore the use of T cells as tools for identifying contact allergens and drugs that may cause health problems is of great interest.
T.O.s Finding Fitness
by Terrell Owens Buddy Primm Courtney ParkerAt the top of his game and his sport, Dallas Cowboys' wide receiver Terrell Owens has a phenomenal body: Muscle & Fitness magazine said this when they chose T. O. for the cover, but anyone who's ever seen him play knows he has the best physique in football. Strong, fast, agile, and focused -- when T. O. takes the field, he's in complete control. Such mastery might seem out of reach for an ordinary athlete or someone just starting to think about getting in shape, but T. O. believes that everyone is c...
T Regulatory Cells in Human Health and Diseases (Advances in Experimental Medicine and Biology #1278)
by Song-Guo ZhengThis book addresses one of the major challenges of immunology today that is being directed to the translation of the rapidly emerging volume of basic science contributions of immunology to clinical medicine. In so doing, the book systemically introduces and discusses concepts, classifications, phenotypic and functional descriptions of regulatory T (Treg) cells in health and disease. The authors of the 15 chapters were selected from among the most qualified experts in the field of Treg cell research who provide a comprehensive overview of Treg cells and their biology in the ensuing chapters.The beginning chapters provide a useful contemporary classification of Treg cell populations and then progress to chapters that explore basic mechanisms of Treg cell function and epigenetic control. In addition to descriptions of typical CD4+ Foxp3+ cells, other chapters provide detailed presentations of Treg subsets such as CD8+ Tregs and IL-10-producing Tr1 cells. The differences of various Treg subsets, as well as circulating and resident Treg cell populations, are next compared. Importantly, the next chapters provide the clinical correlation of Treg cells with autoimmune diseases, inflammatory diseases, metabolic diseases, cancer and organ transplantation and progress to chapters that highlight emerging innovative technology including nanoparticle-Treg cells and their translational values. In summary, the book will provide a valuable resource not only for graduate students and researchers in the fields of immunology, cell biology and translational medicine but also for all others interested in learning more about Treg cells and their application in human health and disease.
T1-Mapping in Myocardial Disease: Principals And Applications
by Phillip C. YangThis book details the advances in cardiac MRI that have enabled quantitative tissue characterization of the myocardium using myocardial and blood T1 measurements, which have enabled reliable detection of diffuse pathological processes in both the cardiomyocytes and the interstitial cells of the myocardium. Evaluation of the native myocardial and interstitial fibrosis, and measurement of the extracellular volume fraction has allowed an unprecedented opportunity to elucidate the pathology, diagnosis and prognosis of cardiovascular disease. T1-Mapping in Myocardial Disease: Principles and Applications reviews a wide spectrum of significant cardiovascular disease and provides relevant guidance for the clinical implementation of this innovative technique. The specific topics covered include principles of T1-mapping in cardiovascular disease and the role of T1-mapping in hypertensive heart disease and hypertrophic cardiomyopathy, cardiotoxicity from cancer treatment, cardiacfibrosis, left ventricular hypertrophy in aortic stenosis, peri-infarct injury in ischemic cardiomyopathy, and stem cell therapy. This comprehensive coverage of the utility of T1-mapping in cardiovascular diseases will greatly appeal to the entire cardiovascular medicine and imaging communities.
Taal: Deel 7 - Handboek Stem-, Spraak- en Taalpathologie
by H.F.M. Peters R. Bastiaanse J. Van Borsel P.H.O. Dejonckere K. Jansonius-Schultheiss Sj. Van der Meulen B.J.E. MondelaersHet Handboek Stem-Spraak-Taalpathologie verscheen tussen 1997 en 2007 gefaseerd in losse afleveringen. Daarin werd alle kennis op het gebied van de stem-, spraak- en taalpathologie vanuit verschillende disciplines samengebracht. Het Handboek is bestemd voor iedereen die klinisch-praktisch of meer theoretisch is geïnteresseerd, of vanuit een ander vakgebied hiermee in aanraking komt. Voor logopedisten, artsen, linguïsten, spraak- en taalpathologen, audiologen, pedagogen en psychologen in Nederland en België is het Handboek een onmisbare vraagbaak.Deel 7 geeft een overzicht van de processen van het produceren en begrijpen van gesproken en geschreven taal. Er is veel aandacht voor de manier waarop kinderen deze processen leren. De taalstoornissen komen in de delen 18, 19 en 20 aan bod.
Taalontwikkelingsstoornissen
by R. Bastiaanse P.H.O. Dejonckere K. Jansonius-Schultheiss B.J.E. Mondelaers H.F.M. Peters J. Van Borsel Sj. Van der MeulenHet Handboek Stem-Spraak-Taalpathologie verscheen tussen 1997 en 2007 gefaseerd in losse afleveringen. Daarin werd alle kennis op het gebied van de stem-, spraak- en taalpathologie vanuit verschillende disciplines samengebracht. Het Handboek is bestemd voor iedereen die klinisch-praktisch of meer theoretisch is geïnteresseerd, of vanuit een ander vakgebied hiermee in aanraking komt. Voor logopedisten, artsen, linguïsten, spraak- en taalpathologen, audiologen, pedagogen en psychologen in Nederland en België is het Handboek een onmisbare vraagbaak.Deel 18 is geheel gewijd aan taalontwikkelingsstoornissen.
Tabakentwöhnung kurz und knackig: Leitfaden zur Raucherentwöhnung für Ärzte, Zahnärzte und andere Gesundheitsberufe (essentials)
by Claudia Bauer-Kemeny Felix HerthDieses Essential plus course möchte allen im Gesundheitswesen tätigen Personen aufzeigen, wie Sie schnell und einfach, aber dennoch effektiv Tabakentwöhnung im Klinik- oder Praxisalltag durchführen können. In Verbindung mit dem dazugehörigen Online-Kurs lernen Sie die Methoden der Kurzintervention kennen, werden über die zur Tabakentwöhnung zugelassenen Medikamente informiert und erfahren, wo und wie entwöhnungswillige Rauchende weitere Unterstützungsangebote erhalten, damit ein Rauchstopp auch wirklich gelingen kann. Den kostenlosen Zugang zum Online-Kurs finden Sie direkt im Buch.
Tackling Causes and Consequences of Health Inequalities: A Practical Guide
by James Matheson John Patterson Laura NeilsonAddressing health inequalities is a key focus for health and social care organizations, yet this is the first book to explore how best frontline health workers in areas of deprivation can address these problems. Aimed at doctors and their wider multidisciplinary teams, this book provides key knowledge and practical advice on how to address the causes and consequences of health inequalities to achieve better outcomes for patients. Considering the psychological, financial and social aspects of well-being as well as health concerns, this book offers a concise but comprehensive overview of the key issues in health inequalities and, most importantly, how practically to address them. Key Features Comprehensively covers the breadth of subjects identified by RCGP’s work to formulate a curriculum for health inequalities The first book to address the urgent area of causes and consequences of health inequalities in clinical practice. Chapters are authored by expert practitioners with proven experience in each aspect of health care. Applied, practical focus, demonstrating approaches that will work and can be applied in ‘every’ situation of inequality. Provides evidence of how community based primary care can make a change.
Tackling Health Inequalities: Reinventing the Role of Environmental Health (Routledge Focus on Environmental Health)
by Surindar Kishen DhesiAlthough environmental health has received some recognition as a field which can positively impact on the social determinants of health, it remains little known outside its immediate sphere of influence. There is also limited literature available to support the potential impact of the profession in public health policy circles, and there has been an overreliance on anecdotal rather than firm evidence. This book presents the findings of an empirical research project focussed on public health policymaking (English Health and Wellbeing Boards), health inequalities and environmental health and provides an insight to the environmental health profession and routes of impact and influence. It discusses environmental health in the context of public health, the role of the profession, issues of visibility and opportunities for impact in today’s policy landscape. In particular, a focus on the local government context is timely given the shifting of the public health function from the National Health Service to local authorities. This book is essential reading for students, practitioners and policymakers in the fields of environmental health and public health.
Tackling HIV-Related Stigma and Discrimination in South Asia
by Dara Carr Laura Nyblade Anne Stangl Laura Brady Mariam Claeson Traci EckhausAlthough overall HIV prevalence in South Asia is low, the widespread stigma attached to HIV and AIDS impedes efforts to reach people most in need of prevention, care, and treatment services. To address this challenge, the 2008 South Asia Region Development Marketplace partnership, led by the World Bank, launched a competitive grants program to support innovative community approaches. 'Tackling HIV-Related Stigma and Discrimination in South Asia' summarizes the monitoring, evaluation, and case study data and documents successful community innovations. Twenty-six community groups in Afghanistan, Bangladesh, India, Nepal, Pakistan, and Sri Lanka received funds. The initiatives involved a broad spectrum, including vulnerable groups as well as people living with HIV, the media, local government authorities, health workers, and religious leaders. The interventions used traditional cultural and media approaches to discuss taboo subjects. The reach of the initiatives was amplifi ed by involving opinion leaders. The strategies engaged marginalized groups to design and lead the interventions and to facilitate contact between groups experiencing stigma and the general public to reduce fears and misconceptions about transmission. Projects that combined economic and stigma reduction interventions helped the marginalized populations to overcome the internalized stigma and become empowered to advocate for their rights. 'Tackling HIV-Related Stigma and Discrimination in South Asia' identifies effective strategies to raise awareness and reports on shifts-albeit slow-of attitudes, norms, and behaviors. Through its recommendations for successful interventions to reduce barriers to effective HIV prevention, care, and treatment programs, the book provides a strong foundation on which to build stigma reduction efforts in the region and world.
Tackling Mental Health Crises
by David Kingdon Marie FinnTackling Mental Health Crises provides a practical guide to combining social and psychological responses to mental health problems. David Kingdon and Marie Finn look at what constitutes a crisis, be it an individual crisis presentation or a situational crisis for services. They reject current diagnostic approaches to mental health, focussing instead on a symptom-led approach and keeping an awareness of the intervention experience for clients and carers throughout. Subjects covered include: engaging and assessing the client risk assessment and management emotional, behavioural, cognitive and physical presentations service teams and settings. The emphasis throughout is on taking a holistic look at crisis prevention and management, encompassing both the work that takes place at an individual level and the wider impact of societal and political issues. Containing clear direction and practical advice, combined with numerous case studies and commentary from users and carers themselves, this book will be of great use to anyone involved with dealing with mental health crises.