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DNA Topoisomerase Protocols, Volume II: Enzymology and Drugs
by Mary-Ann Bjornsti Neil OsheroffDNA Topoisomerase Protocols, II: Enzymology and Drugs brings together an unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods include assays for enzyme-catalyzed DNA relaxation/supercoiling, DNA cleavage, DNA nicking, DNA decatenation, and ATP hydrolysis. Several modified DNA substrates, used to dissect enzyme mechanisms by trapping intermediates, are also described. Methodologies to determine the action of topoisomerase-targeted drugs include biochemical assays of drug-induced enzyme-DNA complexes, methods for assaying drug uptake, and cell-based assays for determining drug specificity and mechanisms of resistance. A companion volume, DNA Topoisomerase Protocols, I: DNA Topology and Enzymes, provides state-of-the-art experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function.<P><P> DNA Topoisomerase Protocols, II: Enzymology and Drugs provides, for the first time, a comprehensive description of the methods and experimental systems needed to investigate fully the catalytic mechanisms of DNA topoisomerases from bacterial, viral, and eukaryotic sources. For the many basic scientists and clinicians who want better to understand and to exploit the action of topoisomerase-targeted drugs in a variety of in vitro and in vivo systems, it will immediately develop immense value as the new standard reference in the field.
DNA Topoisomerases
by Duncan J. ClarkeDue to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise. In DNA Topoisomerases: Methods and Protocols, experts in the field present a compendium of protocols that extends into the new areas of research that have been recently uncovered in order to elucidate the methodology needed to study topoisomerases in the molecular and cellular context. The volume examines subjects such as the analysis of DNA topologies and the interaction of topoisomerases with DNA as tools to measure topoisomerase functions, the binding of topoisomerases to specific sites in the genomes of eukaryotes, including origins of DNA replication, as well as exploration of perturbed topoisomerase function. As a volume in the highly successful Methods in Molecular BiologyTM series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes on trouble-shooting and avoiding known pitfalls. Cutting-edge and easy-to-use, DNA Topoisomerases: Methods and Protocols provides key content which reflects an expanded understanding of the functions of these essential enzymes in the hope of inspiring researchers to further important discoveries in this dynamic field.
DNA Traffic in the Environment
by Hiromi Nishida Taku OshimaThis book comprehensively discusses our current understanding of the role and biological mechanisms of horizontal transfer of genetic elements in the environment, which has been important in the evolution of prokaryotes (archaea and bacteria). Horizontal transfer of genetic elements generates variations of prokaryotes and their genomes. Comparative studies of genomes revealed that it frequently occurred during archaeal and bacterial evolution. The book introduces a variety of studies related to horizontal gene transfer, gene silencing, plasmids, phages, transposons, and the emergence of microbes that degrade man-made xenobiotics and have antimicrobial resistance. Written by leading researchers in DNA traffic, the book is a valuable guide to horizontal transfer for both young scientists and experts in the field.
DNA Tumor Viruses
by James Pipas Blossom DamaniaThis unique book focuses on the DNA viruses in the human population that are associated with cancers. It covers most of the viruses that are thought to contribute to human malignancy. This book represents a comprehensive review of the field of DNA tumor virology. Right now, while there are books out there that cover individual viruses that are also covered in this book, there is no single book that covers this topic comprehensively. This book is the first current, comprehensive review of its kind in the market.
DNA USA: A Genetic Portrait of America
by Bryan SykesCrisscrossing the continent, a renowned geneticist provides a groundbreaking examination of America through its DNA. The best-selling author of The Seven Daughters of Eve now turns his sights on the United States, one of the most genetically variegated countries in the world. From the blue-blooded pockets of old-WASP New England to the vast tribal lands of the Navajo, Bryan Sykes takes us on a historical genetic tour, interviewing genealogists, geneticists, anthropologists, and everyday Americans with compelling ancestral stories. His findings suggest: * Of Americans whose ancestors came as slaves, virtually all have some European DNA. * Racial intermixing appears least common among descendants of early New England colonists. * There is clear evidence of Jewish genes among descendants of southwestern Spanish Catholics. * Among white Americans, evidence of African DNA is most common in the South. * European genes appeared among Native Americans as early as ten thousand years ago. An unprecedented look into America's genetic mosaic and how we perceive race, DNA USA challenges the very notion of what we think it means to be American.
DNA Vaccines
by Monica Rinaldi Daniela Fioretti Sandra IuresciaDNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines. Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease.
Do Animals Think?
by Clive D. WynneDoes your dog know when you've had a bad day? Can your cat tell that the coffee pot you left on might start a fire? Could a chimpanzee be trained to program your computer? In this provocative book, noted animal expert Clive Wynne debunks some commonly held notions about our furry friends. It may be romantic to ascribe human qualities to critters, he argues, but it's not very realistic. While animals are by no means dumb, they don't think the same way we do. Contrary to what many popular television shows would have us believe, animals have neither the "theory-of-mind" capabilities that humans have (that is, they are not conscious of what others are thinking) nor the capacity for higher-level reasoning. So, in Wynne's view, when Fido greets your arrival by nudging your leg, he's more apt to be asking for dinner than commiserating with your job stress. That's not to say that animals don't possess remarkable abilities--and Do Animals Think? explores countless examples: there's the honeybee, which not only remembers where it found food but communicates this information to its hivemates through an elaborate dance. And how about the sonar-guided bat, which locates flying insects in the dark of night and devours lunch on the wing? Engagingly written, Do Animals Think? takes aim at the work of such renowned animal rights advocates as Peter Singer and Jane Goodall for falsely humanizing animals. Far from impoverishing our view of the animal kingdom, however, it underscores how the world is richer for having such a diversity of minds--be they of the animal or human variety.
Do Chocolate Lovers Have Sweeter Babies?: The Surprising Science of Pregnancy
by Jena PincottBrain Candy for expectant parents! Pregnancy is an adventure. Lots of books tell you the basics—“the baby is the size of [insert fruit here].” But pregnant science writer Jena Pincott began to wonder just how a baby might tinker with her body—and vice versa—and chased down answers to the questions she wouldn’t ask her doctor, such as: • Does stress sharpen your baby’s mind—or dull it? • Can you predict your baby’s temperament? • Why are babies born in the darker months of the year more likely to grow up to be novelty-loving risk takers? • Are bossy, dominant women more likely to have boys? • How can the cells left behind by your baby affect you years later? This is a different kind of pregnancy book—thoughtful, fun, and filled with information you won’t find anywhere else.
Do Dice Play God?: The Mathematics of Uncertainty
by Ian StewartA celebrated mathematician explores how math helps us make sense of the unpredictableWe would like to believe we can know things for certain. We want to be able to figure out who will win an election, if the stock market will crash, or if a suspect definitely committed a crime. But the odds are not in our favor. Life is full of uncertainty --- indeed, scientific advances indicate that the universe might be fundamentally inexact --- and humans are terrible at guessing. When asked to predict the outcome of a chance event, we are almost always wrong. Thankfully, there is hope. As award-winning mathematician Ian Stewart reveals, over the course of history, mathematics has given us some of the tools we need to better manage the uncertainty that pervades our lives. From forecasting, to medical research, to figuring out how to win Let's Make a Deal, Do Dice Play God? is a surprising and satisfying tour of what we can know, and what we never will.
Do Gentlemen Really Prefer Blondes? Why He Fancies You and Why He Doesn't
by Jena PincottQ: Do Gentlemen Really Prefer Blondes? A: Marilyn Monroe, Scarlet Johansson and Gwyneth Paltrow would be happy to know that they do. During the Ice Age, when even cavemen were in short supply, the blonde woman really did get her man - simply because her light coloured hair made her stand out. Plus scientists have recently discovered that natural blondes have higher oestrogen levels. In short, golden (preferably long) hair shouts : 'I am young, sexy - and fertile' to every member of the male species within a few miles. Q: When's the best time to seduce my man? A: Your other half will definitely get more possessive and more attracted to you when you're ovulating and at your most fertile. You'll spend time on your make-up and choose next day's outfit before you go to bed. Your lips will be fuller, and your skin will be radiant because of the high levels of oestrogen being pumped through your blood. But don't get carried away by how sexy you feel. You are also more likely to be unfaithful during this period and your boyfriend is also likely to run away with another equally fertile member of the female species. Q: How do I get him to change his wild lifestyle? A: Get pregnant. Shocking but true. Fathers have lower testosterone levels than single men which means that they are more easy-going and less confrontational, they take fewer life threatening risks, and they are far less likely to have an affair with the leggier, skinnier blonde from the office.
Do Humankind’s Best Days Lie Ahead?: The Munk Debates (The Munk Debates #2015)
by Steven Pinker Matt Ridley Alain de Botton Malcolm GladwellProgress. It is one of the animating concepts of the modern era. From the Enlightenment onwards, the West has had an enduring belief that through the evolution of institutions, innovations, and ideas, the human condition is improving. This process is supposedly accelerating as new technologies, individual freedoms, and the spread of global norms empower individuals and societies around the world. But is progress inevitable? Its critics argue that human civilization has become different, not better, over the last two and a half centuries. What is seen as a breakthrough or innovation in one period becomes a setback or limitation in another. In short, progress is an ideology not a fact; a way of thinking about the world as opposed to a description of reality.In the seventeenth semi-annual Munk Debates, which was held in Toronto on November 6, 2015, pioneering cognitive scientist Steven Pinker and bestselling author Matt Ridley squared off against noted philosopher Alain de Botton and bestselling author Malcolm Gladwell to debate whether humankind’s best days lie ahead.
Do I Know You?: A Faceblind Reporter's Journey into the Science of Sight, Memory, and Imagination
by Sadie DingfelderAn award-winning science writer discovers she&’s faceblind and investigates the neuroscience of sight, memory, and imagination—while solving some long-running mysteries about her own life. Science writer Sadie Dingfelder has always known that she&’s a little quirky. But while she&’s made some strange mistakes over the years, it&’s not until she accosts a stranger in a grocery store (whom she thinks is her husband) that she realizes something is amiss. With a mixture of curiosity and dread, Dingfelder starts contacting neuroscientists and lands herself in scores of studies. In the course of her nerdy midlife crisis, she discovers that she is emphatically not neurotypical. She has prosopagnosia (faceblindness), stereoblindness, aphantasia (an inability to create mental imagery), and a condition called severely deficient autobiographical memory. As Dingfelder begins to see herself more clearly, she discovers a vast well of hidden neurodiversity in the world at large. There are so many different flavors of human consciousness, and most of us just assume that ours is the norm. Can you visualize? Do you have an inner monologue? Are you always 100 percent sure whether you know someone or not? If you can perform any of these mental feats, you may be surprised to learn that many people—including Dingfelder—can&’t. A lively blend of personal narrative and popular science, Do I Know You? is the story of one unusual mind&’s attempt to understand itself—and a fascinating exploration of the remarkable breadth of human experience.
Do No Harm: Protecting Connected Medical Devices, Healthcare, and Data from Hackers and Adversarial Nation States
by Matthew WebsterDiscover the security risks that accompany the widespread adoption of new medical devices and how to mitigate them In Do No Harm: Protecting Connected Medical Devices, Healthcare, and Data from Hackers and Adversarial Nation States, cybersecurity expert Matthew Webster delivers an insightful synthesis of the health benefits of the Internet of Medical Things (IoMT), the evolution of security risks that have accompanied the growth of those devices, and practical steps we can take to protect ourselves, our data, and our hospitals from harm. You’ll learn how the high barriers to entry for innovation in the field of healthcare are impeding necessary change and how innovation accessibility must be balanced against regulatory compliance and privacy to ensure safety. In this important book, the author describes: The increasing expansion of medical devices and the dark side of the high demand for medical devices The medical device regulatory landscape and the dilemmas hospitals find themselves in with respect medical devices Practical steps that individuals and businesses can take to encourage the adoption of safe and helpful medical devices or mitigate the risk of having insecure medical devices How to help individuals determine the difference between protected health information and the information from health devices--and protecting your data How to protect your health information from cell phones and applications that may push the boundaries of personal privacy Why cybercriminals can act with relative impunity against hospitals and other organizations Perfect for healthcare professionals, system administrators, and medical device researchers and developers, Do No Harm is an indispensable resource for anyone interested in the intersection of patient privacy, cybersecurity, and the world of Internet of Medical Things.
Do Not Lick This Book
by Idan Ben-BarakIn this interactive children’s picture book, microbiologist Idan Ben-Barak and award-winning illustrator Julian Frost zoom in on the microscopic world found on everyday objects—and in our bodies—warning readers Do Not Lick This Book.Min is a microbe. She is small.Very small.In fact, so small that you’d need to look through a microscope to see her. Or you can simply open this book and take Min on an adventure to amazing places she’s never seen before—like the icy glaciers of your tooth or the twisted, tangled jungle of your shirt.
Do Plants Know Math?: Unwinding the Story of Plant Spirals, from Leonardo da Vinci to Now
by Stéphane Douady Jacques Dumais Christophe Golé Nancy PickA breathtakingly illustrated look at botanical spirals and the scientists who puzzled over themCharles Darwin was driven to distraction by plant spirals, growing so exasperated that he once begged a friend to explain the mystery &“if you wish to save me from a miserable death.&” The legendary naturalist was hardly alone in feeling tormented by these patterns. Plant spirals captured the gaze of Leonardo da Vinci and became Alan Turing&’s final obsession. This book tells the stories of the physicists, mathematicians, and biologists who found themselves magnetically drawn to Fibonacci spirals in plants, seeking an answer to why these beautiful and seductive patterns occur in botanical forms as diverse as pine cones, cabbages, and sunflowers.Do Plants Know Math? takes you down through the centuries to explore how great minds have been captivated and mystified by Fibonacci patterns in nature. It presents a powerful new geometrical solution, little known outside of scientific circles, that sheds light on why regular and irregular spiral patterns occur. Along the way, the book discusses related plant geometries such as fractals and the fascinating way that leaves are folded inside of buds. Your neurons will crackle as you begin to see the connections. This book will inspire you to look at botanical patterns—and the natural world itself—with new eyes.Featuring hundreds of gorgeous color images, Do Plants Know Math? includes a dozen creative hands-on activities and even spiral-plant recipes, encouraging readers to explore and celebrate these beguiling patterns for themselves.
Do Polar Bears Get Lonely?: And 101 Other Intriguing Science Questions
by New ScientistDo Polar Bears Get Lonely? is the third compilation of readers' answers to the questions in the 'Last Word' column of New Scientist, the world's best-selling science weekly. Following the phenomenal success of Does Anything Eat Wasps? (2005) and the even more spectacularly successful Why Don't Penguins' Feet Freeze? (2006), this latest collection includes a bumper crop of wise and wonderful answers never before seen in book form.As usual, the simplest questions often have the most complex answers - while some that seem the knottiest have very simple explanations. New Scientist's 'Last Word' is regularly voted the magazine's most popular section as it celebrates all questions - the trivial, idiosyncratic, baffling and strange. This all-new and eagerly awaited selection of the best again presents popular science at its most entertaining and enlightening.
Do Polar Bears Get Lonely: And 101 other intriguing science questions
by New ScientistDo Polar Bears Get Lonely? is the third compilation of readers' answers to the questions in the 'Last Word' column of New Scientist, the world's best-selling science weekly. Following the phenomenal success of Does Anything Eat Wasps? (2005) and the even more spectacularly successful Why Don't Penguins' Feet Freeze? (2006), Do Polar Bears Get Lonely? includes a bumper crop of wise and wonderful questions and answers such as:Why does garlic make your breath smell? How toothpaste makers get the stripes in toothpaste? Why do we get 'pins and needles'? Why are some people left-handed and other people right-handed? Can insects get fat? Do elephants sneeze? And do fish get thirsty? What causes cells to stick together in the human body rather than simply fall apart? And why are pears pear-shaped (and not apple-shaped)?This eagerly awaited selection of the best once again presents popular science at its most entertaining and enlightening.
Do Polar Bears Get Lonely?: And 101 Other Intriguing Science Questions
by New ScientistDo Polar Bears Get Lonely? is the third compilation of readers' answers to the questions in the 'Last Word' column of New Scientist, the world's best-selling science weekly. Following the phenomenal success of Does Anything Eat Wasps? (2005) and the even more spectacularly successful Why Don't Penguins' Feet Freeze? (2006), this latest collection includes a bumper crop of wise and wonderful answers never before seen in book form.As usual, the simplest questions often have the most complex answers - while some that seem the knottiest have very simple explanations. New Scientist's 'Last Word' is regularly voted the magazine's most popular section as it celebrates all questions - the trivial, idiosyncratic, baffling and strange. This all-new and eagerly awaited selection of the best again presents popular science at its most entertaining and enlightening.
Do Robots Make Love?: From AI to Immortality – Understanding Transhumanism in 12 Questions
by Laurent Alexandre Jean-Michel BesnierShould we enhance the human condition with technology?Does anyone really want to live for a thousand years?Could AI end up destroying mankind?Discover the incredible potential of mankind's near future as Doctor and entrepreneur Laurent Alexandre and tech-philosopher Jean Michel Besnier go head to head on the big questions in an entertaining and thought-provoking debate on the fundamental principles of transhumanism.This movement seeks to improve the human condition through science - has fast become one of the most controversial the scientific community have ever faced. As great strides are made in using advanced technology to enhance human intellect and physiology, the ethical and moral questions surrounding its possibilities have never been more pressing. Should we change the way we reproduce? Could we enhance the human body with technology to the point where we are all technically cyborgs? Is it possible to make love to a robot?
Do Something: Activism for Everyone
by Kajal Odedra'An indispensable manual for budding activists by one of the country's most effective campaigners.'Cathy Newman, Journalist and Presenter'Tired of complaining but don't know what to do? This beautifully written book will not only inspire you but give you a step-by-step guide to creating positive change.'Magid Magid, Politician and Activist'This is your mayday book. If you want to start your own resistance, buy Do Something.'Deborah Frances-White, Host of The Guilty Feminist podcastDo you find yourself staring helplessly at your news feed? Or all too often asking, 'why hasn't somebody done something about that?'. If the answer is yes, then DO SOMETHING is the book you need.Whether you simply want to challenge your local shop to reduce their plastic or go big and demand a new law to be passed, this book is the place to start.Full of lessons from the real world DO SOMETHING contains practical steps and a blueprint anyone can follow - from helping you to pinpoint the fundamentals of what you want to achieve to mobilising supporters and harnessing traditional and social media. Having worked as a campaigner for over a decade Kajal Odedra knows the tricks that have typically been held by people in circles of power and believes that everyone should know how to speak up and be heard. Revolution on every scale is happening all around the world - but rather than being led by governments, policy makers or political leaders, it is individuals, communities and collectives who are calling for action. People power works! So, stop banging your head against the wall, pick up this book, and start planning your resistance.
Do Something: Activism for Everyone
by Kajal Odedra'An indispensable manual for budding activists by one of the country's most effective campaigners.'Cathy Newman, Journalist and Presenter'Tired of complaining but don't know what to do? This beautifully written book will not only inspire you but give you a step-by-step guide to creating positive change.'Magid Magid, Politician and Activist'This is your mayday book. If you want to start your own resistance, buy Do Something.'Deborah Frances-White, Host of The Guilty Feminist podcastDo you find yourself staring helplessly at your news feed? Or all too often asking, 'why hasn't somebody done something about that?'. If the answer is yes, then DO SOMETHING is the book you need.Whether you simply want to challenge your local shop to reduce their plastic or go big and demand a new law to be passed, this book is the place to start.Full of lessons from the real world DO SOMETHING contains practical steps and a blueprint anyone can follow - from helping you to pinpoint the fundamentals of what you want to achieve to mobilising supporters and harnessing traditional and social media. Having worked as a campaigner for over a decade Kajal Odedra knows the tricks that have typically been held by people in circles of power and believes that everyone should know how to speak up and be heard. Revolution on every scale is happening all around the world - but rather than being led by governments, policy makers or political leaders, it is individuals, communities and collectives who are calling for action. People power works! So, stop banging your head against the wall, pick up this book, and start planning your resistance.
Do Something: Activism for Everyone
by Kajal OdedraA user's guide to Activism by one of the UK's biggest names in grass-roots campaigning shows in a few simple steps how we can all make a difference.People power works and is changing the world around us. This is a user's guide to activism by one of the UK's biggest names in grass-roots campaigning. Illustrated with lessons from the real world, it is a guide to creating change for anyone who has ever asked themselves 'why hasn't anyone done something about that?'We are taught to believe that only a small group of people in society have the ability to change laws and company policies. That's simply not true any longer - each of is capable of using our experiences to change the world. We just need to use that thing that makes us different, and learn how to channel it. Having worked as a campaigner for the last 11 years, Kajal Odedra of change.org - which is used by 15 million people in the UK - has learned the tricks of the trade that have traditionally been held in circles of power, and believes that everyone should know how to speak up and be heard. Her mission is to show people how to use their voice to make their community, politicians and CEOs take notice.(P)2019 Hodder & Stoughton Ltd
Do Sparrows Like Bach?: The Strange and Wonderful Things that Are Discovered When Scientists Break Free
by New ScientistFrom the same editors that brought you Why Don't Penguins' Feet Freeze? and Does Anything Eat Wasps?, an exploration of the weird and wonderful margins of science--the latest volume in the brilliant New Scientist series. Science tells us grand things about the universe: how fast light travels, and why stones fall to earth. But scientific endeavor goes far beyond these obvious foundations. There are some fields we don`t often hear about because they are so specialized, or turn out to be dead ends. Yet researchers have given hallucinogenic drugs to blind people (seriously), tried to weigh the soul as it departs the body, and planned to blast a new Panama Canal with an atomic weapon. Real scientific breakthroughs sometimes come out of the most surprising and unpromising work. Do Sparrows Like Bach? is about the margins of science--investigating everything from what it`s like to die to exploding pants and recycled urine. Who on earth would burn off their beard with a laser? Produce a fireproof umbrella that doubles as a parachute? Replace sniffer dogs with gerbils? Could a chemical component of flatulence be the next Viagra? Do sparrows (and even fish for that matter) prefer Bach to Led Zeppelin? The editors at New Scientist magazine have the answers to all these questions and more in this celebration of outrageous, outlandish, and brilliant discoveries on the fringes of scientific research. This extraordinary collection is an astonishing reminder that even at its most misguided, science is intensely creative, often hilarious, and can spark the imagination like nothing else.
Do Species Exist?: Principles of Taxonomic Classification
by Werner KunzA readily comprehensible guide for biologists, field taxonomists and interested laymen to one of the oldest problems in biology: the species problem. Written by a geneticist with extensive experience in field taxonomy, this practical book provides the sound scientific background to the problems arising with classifying organisms according to species. It covers the main current theories of specification and gives a number of examples that cannot be explained by any single theory alone.