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DNA Fingerprinting: Advancements and Future Endeavors
by Hirak Ranjan Dash Pankaj Shrivastava Braja Kishore Mohapatra Surajit DasThis book describes the basics and various applications of DNA fingerprinting, including in actual case studies. The book is divided in four modules; Module 1: Basics of DNA Fingerprinting, Module 2: Applications of DNA Fingerprinting, Module 3: DNA Fingerprinting: Case Studies, and Module 4: Future of DNA Fingerprinting. Each module consists of 4 to 5 chapters, written by reputed researchers, academics and forensic scientists from around the globe. The respective chapters cover e.g. related fields, the tools and techniques used, various genotyping kits, real-world case studies, ancient DNA and wild life forensics, molecular diagnosis of human diseases, legal aspects, microbial forensics and the economics of the DNA fingerprinting technique. The book offers a practical guide for professionals, graduate and post-graduate students in the fields of Forensic Science, Medicine, Genetics, Anthropology, Microbiology, and Zoology. It also serves as a useful reference resource, summarizing major technological advances in the field of DNA fingerprinting, the problems faced in this field of science and possible new solutions to these problems. Presently, DNA fingerprinting is utilized in solving the majority of criminal cases; as such, the book is also helpful for investigating agencies, as it includes representative case studies.
DNA Fingerprinting
by M. Krawczak J. SchmidtkeThe book is primarily concerned with DNA fingerprinting and DNA profiling in the context of forensic medicine and kinship testing. It concentrates on methods of determining the degree of relatedness of members of the same species, focusing on humans and occasionally glancing at other species.
DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition
by Kurt Weising Hilde Nybom Markus Pfenninger Kirsten Wolff Günter KahlGiven the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated p
DNA Helicases and DNA Motor Proteins (Advances in Experimental Medicine and Biology #767)
by Maria SpiesIn recent years, a number of groundbreaking structural and mechanistic studies deepened our understanding of helicase mechanisms and established new approaches for their analyses. Many fundamental mechanistic questions ranging from the mechanism of force generation, mechanochemical coupling to distinct mechanisms by which the same enzyme translocates on DNA removing obstacles, unwinds DNA and/or remodels nucleoprotein complexes, however, remain to be answered. It is even less understood how the helicase motors are incorporated into a wide range of genome maintenance and repair machines. The field has reached a stage when the studies of molecular mechanisms and basic biology of helicases can and shall be integrated with the studies of development, cancer and longevity. The objective of this book is to provide the first systematic overview of structure, function and regulation of DNA helicases and related molecular motors. By integrating the knowledge obtained through the diverse technical approaches ranging from single-molecule biophysics to cellular and molecular biological studies the editors aim to provide a unified view on how helicases function in the cell, are regulated in response to different cellular stresses and are integrated into large macromolecular assemblies to form a complex and adaptive living system.
DNA Information: Laws of Perception
by Georgi MuskhelishviliThis book explores the double coding property of DNA, which is manifested in the digital and analog information types as two interdependent codes. This double coding principle can be applied to all living systems, from the level of the individual cell to entire social systems, seen as systems of communication. Further topics discussed include the ubiquitous problem of logical typing, which reflects our inherent incapacity to simultaneously perceive discontinuity and continuity, the problem of time, and the peculiarities of autopoietic living systems. It is shown that the scientific "truths" that appear to be coherent constructions connecting the scientifically verified observations by the rules of logic are in fact always relative and never absolute.
DNA is Not Destiny
by Steven J. HeineDo you fear what might be lurking in your DNA?Well, now you can find out, and you most likely will. Scientists expect one billion people to have their genomes sequenced by 2025, and as the price drops it may even become a standard medical procedure. Yet cultural psychologist Steven Heine argues that the first thing we'll do upon receiving our DNA test results is to misinterpret them completely. We've become accustomed to breathless media coverage about newly discovered "cancer" or "IQ" or "infidelity" genes, each one promising a deeper understanding of what makes us tick. But as Heine shows, most of these claims are oversimplified and overhyped misinterpretations of how our DNA really works. With few exceptions, it is a complex combination of experience, environment, and genetics that determines who we are, how we behave, and what diseases will afflict us in the future. So why do we continue to buy into the belief that our genes control our destiny? Heine argues that we are psychologically ill equipped to deal with DNA results, repeatedly falling into predictable biases--switch-thinking, essentialism, fatalism, negativity dominance, and more--that mold our thinking about the information we receive. Heine shares his research--and his own genome-sequencing results--to not only to set the record straight regarding what your genes actually reveal about your health, intelligence, ethnic identity, and family, but to also help you counteract these insidious cognitive traps. His fresh, surprising conclusions about the promise, and limits, of genetic engineering and DNA testing upend conventional thinking and reveal a simple, profound truth: your genes create life--but they do not control it.
DNA Know Thyself: Living in a DNA World
by Ray J RoseThe book provides a concise, integrated view of DNA biology and biotechnology in various aspects, such as human health, food production, environmental sustainability, and industry development. It covers the evolution of DNA, genes, cancer, aging biology, and plant gene technology. The chapters delve into the evolution of DNA, cells, genes, and regulation and its influence on cancer, aging, and development. The book emphasizes modern DNA biotechnology, including plant gene technology, gene therapy, and synthetic biology, and highlights the relevance of DNA knowledge in addressing global challenges. It focuses on providing a contemporary understanding and the background of DNA biology, making it suitable for science and medicine programs or as an introduction to specialized DNA/biotechnology areas. The book targets academicians, researchers, and clinicians in related fields. It is also a valuable resource for anyone interested in understanding DNA and its applications in various fields.
DNA Liquid-Crystalline Dispersions and Nanoconstructions (Liquid Crystals Book Series)
by Yuri M. Yevdokimov V.I. Salyanov S.V. Semenov S.G. SkuridinThe discovery of the spatial structure of the double-stranded DNA molecule is one of the greatest achievements of science. It would not be an exaggeration to say that the DNA double helix is a distinguished symbol of modern biology. Divided into three parts, DNA Liquid-Crystalline Dispersions and Nanoconstructions covers the information presently a
DNA Manipulation and Analysis (Methods in Molecular Biology #2633)
by Garry ScarlettThis volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Gene Modification and Nucleic Acid Technologies aims to be the comprehensive guide for life scientists moving into the field of recombinant DNA and nucleic acid manipulation.
DNA Methylation: Approaches, Methods, and Applications
by Manel EstellerDNA Methylation: Approaches, Methods and Applications describes the relation DNA methylation has to gene silencing in disease, and explores its promising role in treating cancer. Written by leaders in the field, this exceptional compilation of articles outlines the best techniques to use when addressing questions concerning the cytosine methylation
DNA Methylation Microarrays: Experimental Design and Statistical Analysis
by Sun-Chong Wang Art PetronisProviding an interface between dry-bench bioinformaticians and wet-lab biologists, DNA Methylation Microarrays: Experimental Design and Statistical Analysis presents the statistical methods and tools to analyze high-throughput epigenomic data, in particular, DNA methylation microarray data. Since these microarrays share the same under
DNA Methylation Protocols
by Bernie H. Ramsahoye Ken I. MillsDNA Methylation Protocols offer a set of readily reproducible protocols of the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation and the isolation and measurement of demethylases and related proteins.
DNA Methyltransferases - Role and Function
by Albert Jeltsch Renata Z. JurkowskaDNA methyltransferases are important enzymes in a broad range of organisms. Dysfunction of DNA methyltransferases in humans leads to many severe diseases, including cancer. This book focuses on the biochemical properties of these enzymes, describing their structures and mechanisms in bacteria, humans and other species, including plants, and also explains the biological processes of reading of DNA methylation and DNA demethylation. It covers many emerging aspects of the biological roles of DNA methylation functioning as an essential epigenetic mark and describes the role of DNA methylation in diseases. Moreover, the book explains modern technologies, like targeted rewriting of DNA methylation by designed DNA methyltransferases, as well as technological applications of DNA methyltransferases in DNA labelling. Finally, the book summarizes recent methods for the analysis of DNA methylation in human DNA. Overall, this book represents a comprehensive state-of-the-art- work and is a must-have for advanced researchers in the field of DNA methylation and epigenetics.
DNA Methyltransferases - Role and Function (Advances in Experimental Medicine and Biology #1389)
by Albert Jeltsch Renata Z. JurkowskaThis 2nd edition of the book on DNA methyltransferases has been comprehensively updated to reflect many novel research findings regarding the structure, function, and technology of these enzymes that have emerged over the past 6 years.Like the previous edition, this 2nd edition explains the biochemical properties of DNA methyltransferases, describing their structures, mechanisms and biological roles in bacteria, humans and plants. It also discusses the biological processes of reading DNA methylation and the mechanisms of DNA demethylation. This volume highlights the newest findings on DNA methyltransferase inhibitors and their use in cancer therapy as well as the latest epigenome editing systems based on these enzymes. Overall, this 2nd edition comprehensively summarizes the current state of research in the field of DNA methylation and DNA methyltransferase and is essential reading for early career and advanced researchers in this exciting field.
DNA Microarrays (Advanced Methods)
by Ulrike A NuberDNA Microarrays introduces all up-to-date microarray platforms and their various applications. It is written for scientists who are entering the field of DNA microarrays as well as those already familiar with the technology, but interested in new applications and methods.
DNA Microarrays and Related Genomics Techniques: Design, Analysis, and Interpretation of Experiments
by David B. Allison T. Mark Beasley Jode W. EdwardsConsidered highly exotic tools as recently as the late 1990s, microarrays are now ubiquitous in biological research. Traditional statistical approaches to design and analysis were not developed to handle the high-dimensional, small sample problems posed by microarrays. In just a few short years the number of statistical papers providing approaches
DNA Modification Detection Methods (Springer Protocols Handbooks)
by Bi-Feng YuanUnderstanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years. This book provides a comprehensive overview of available techniques and methods together with detailed step-by-step protocols for experimental procedures required to successfully perform analysis on various types of DNA modifications, including 5mC, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU), 5-formyluracil (5fU), N6-methyladenine (6mA), β-D-glucosyl-5-hydroxymethyluracil (base J) and 8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable source for biochemists and molecular biologists from different fields who wish to investigate DNA modifications.
DNA Modifications: Methods and Protocols (Methods in Molecular Biology #2198)
by Alexey Ruzov Martin GeringThis book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techniques for the detection and quantification of DNA modifications, antibody-based approaches to study their spatial distribution in different cells and tissues, and methods to analyze their genomic distribution with the help of bioinformatics tools that interrogate the corresponding datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, DNA Modifications: Methods and Protocols serves as an ideal guide to research scientists and PhD students in this rapidly developing discipline, and, thus, will ultimately contribute to deciphering the roles of non-canonical DNA nucleotides in different biological systems.
DNA Nanoscience: From Prebiotic Origins to Emerging Nanotechnology
by Kenneth DouglasDNA Nanoscience: From Prebiotic Origins to Emerging Nanotechnology melds two tales of DNA. One is a look at the first 35 years of DNA nanotechnology to better appreciate what lies ahead in this emerging field. The other story looks back 4 billion years to the possible origins of DNA which are shrouded in mystery. The book is divided into three parts comprised of 15 chapters and two Brief Interludes. Part I includes subjects underpinning the book such as a primer on DNA, the broader discipline of nanoscience, and experimental tools used by the principals in the narrative. Part II examines the field of structural DNA nanotechnology, founded by biochemist/crystallographer Nadrian Seeman, that uses DNA as a construction material for nanoscale structures and devices, rather than as a genetic material. Part III looks at the work of physicists Noel Clark and Tommaso Bellini who found that short DNA (nanoDNA) forms liquid crystals that act as a structural gatekeeper, orchestrating a series of self-assembly processes using nanoDNA. This led to an explanation of the polymeric structure of DNA and of how life may have emerged from the prebiotic clutter.
DNA Nanotechnology
by Chunhai FanDNA nanotechnology: From structure to function presents an overview of various facets of DNA nanotechnology, with a particular focus on their promising applications. This book is composed of three parts. Part I, Elements of DNA Nanotechnology, provides extensive basic information on DNA nanotechnology. Part II, Static and Dynamic DNA Nanotechnology, describes the design and fabrication of static and dynamic DNA nanostructures. Recent advances in DNA origami, DNA walkers and DNA nanodevices are all covered in this part. Part III, Applications of DNA Nanotechnology, introduces a variety of applications of DNA nanotechnology, including biosensing, computation, drug delivery, etc. Together these provide a comprehensive overview of this emerging area and its broad impact on biological and medical sciences. This book is intended for post-graduates, post-doctoral researchers and research scientists who are interested in expanding their knowledge of DNA nanotechnology. It provides readers an impression of the latest developments in this exciting filed.
DNA Nanotechnology
by Bruno Samorì Giampaolo ZuccheriCurrently, nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the biological behavior and function of DNA. With the structural perfection of a self-assembling DNA nano-object, such as a DNA origami, it is clear how complex DNA is as a molecule, leading researchers to wonder how many different constructs could be designed and realized. DNA Nanotechnology: Methods and Protocols shows the procedures to follow in order to repeat methods that lead to such constructs or to the mastering of the characterization techniques used to study them. The chapters of this book are roughly divided into two parts: some cover the methods for preparing the nanostructures, from the rationale of the operations to the techniques for their handling, while other chapters deal more directly with advanced instrumental techniques that can manipulate and characterize molecules and nanostructures. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, DNA Nanotechnology: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to ignite interest and spur activity in this young and rapidly growing research field. Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
DNA Nanotechnology: Methods and Protocols (Methods in Molecular Biology #1811)
by Giampaolo ZuccheriThis second edition provides new and updated methods that detail new DNA nanotechnology techniques. Chapters focus on DNA origami nanostructures for arranging matter in the nanoscale or on their manipulation with the aid of other technologies, on procedures for making nucleic acids nanostructures of different kinds, and methods to simulate complex nanostructures or to use them in biosensing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, DNA Nanotechnology: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
DNA Nanotechnology for Cell Research: From Bioanalysis to Biomedicine
by Zhou NieDNA Nanotechnology for Cell Research Comprehensive coverage of DNA nanotechnology with a focus on its biomedical applications in disease diagnosis, gene therapy, and drug delivery Bringing together multidisciplinary aspects of chemical, material, and biological engineering, DNA Nanotechnology for Cell Research: From Bioanalysis to Biomedicine presents an overview of DNA nanotechnology with emphasis on a variety of different applications in cell research and engineering, covering a unique collection of DNA nanotechnology for fundamental research and engineering of living cells, mostly in cellulo and in vivo, for the first time. Broad coverage of this book ranges from pioneering concepts of DNA nanotechnology to cutting-edge reports regarding the use of DNA nanotechnology for fundamental cell science and related biomedical engineering applications in sensing, bioimaging, cell manipulation, gene therapy, and drug delivery. The text is divided into four parts. Part I surveys the progress of functional DNA nanotechnology tools for cellular recognition. Part II illustrates the use of DNA-based biochemical sensors to monitor and image intracellular molecules and processes. Part III examines the use of DNA to regulate biological functions of individual cells. Part IV elucidates the use of DNA nanotechnology for cell-targeted medical applications. Sample topics covered in DNA Nanotechnology for Cell Research include: Selections and applications of functional nucleic acid toolkits, including DNA/RNA aptamers, DNAzymes, and riboswitches, for cellular recognition, metabolite detection, and liquid biopsy. Developing intelligent DNA nanodevices implemented in living cells for amplified cell imaging, smart intracellular sensing, and in cellulo programmable biocomputing. Harnessing dynamic DNA nanotechnology for non-genetic cell membrane engineering, receptor signaling reprogramming, and cellular behavior regulation. Construction of biocompatible nucleic acid nanostructures as precisely controlled vehicles for drug delivery, immunotherapy, and tissue engineering. Providing an up-to-date tutorial style overview along with a highly valuable in-depth perspective, DNA Nanotechnology for Cell Research is an essential resource for the entire DNA-based nanotechnology community, including analytical chemists, biochemists, materials scientists, and bioengineers.
DNA Recombination
by Hideo TsubouchiHomologous recombination is important in various aspects of DNA metabolism, including damage repair, replication, telomere maintenance, and meiosis, and yeast genetics has successfully provided a framework for the mechanism of homologous recombination. Divided into four convenient sections, DNA Recombination: Methods and Protocols covers recent techniques that best utilize the advantages of the yeast system, prescribing to the belief that yeast will keep serving as a great model organism to study homologous recombination. Chapters have also been included for such exceptions as the group of genes involved in recombination that are found solely in higher eukaryotes, such as BRCA2. And looking forward, a necessary step in the direction of understanding the homologous recombination process is to isolate the machine and let it work in a test tube. Understanding the design by studying the appearance and behavior of the machinery as a single molecule will be an important milestone toward understanding the mechanism of action of the machinery. Techniques covering these topics have also been included. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, DNA Recombination: Methods and Protocols serves as an ideal guide to scientists of all backgrounds with its well-honed methodologies and strives to bring the reader to the next level of understanding regarding this vital subject.
DNA Repair and Replication: Mechanisms and Clinical Significance
by Roger J. Grand John J. ReynoldsDNA Repair and Replication brings together contributions from active researchers. The first part of this book covers most aspects of the DNA damage response, emphasizing the relationship to replication stress. The second part concentrates on the relevance of this to human disease, with particular focus on both the causes and treatments which make use of DNA Damage Repair (DDR) pathways.