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Histamine Receptors
by Patrizio Blandina Maria Beatrice PassaniA comprehensive and detailed overview of the current state of preclinical research on histamine and histamine receptors. Part of the book focuses on novel approaches to the study of histamine receptors such as polymorphism, genetic linkage, and computational analysis, and on the use of new histaminergic ligands in diseases such as asthma and dermatitis. Several chapters will be devoted to the role of histamine in the control of homeostatic and behavioral responses such as the sleep-wake cycle, regulation of the blood brain barrier, food intake, alertness, itch, and memory formation and consolidation.
Histamine Receptors as Drug Targets
by Ekaterini Tiligada Madeleine EnnisThis book illustrates the current state-of-the-art in histamine research, with a focus on the appropriate methodologies to investigate the pharmacological properties and the therapeutic exploitation of HRs and their ligands. In addition, the range of techniques described provides an introduction to complementary cross-methodological disciplines beyond these fields. This multi-disciplinary approach is required to define the 'decision gates' that determine the development of more effective and safer therapeutic options for many forms of highly prevalent and debilitating diseases, such as asthma, dementias, dermatitis, and arthritis. Written for the Methods in Pharmacology and Toxicology series, chapters concentrate on practical, hands-on protocols from experts in the techniques. Authoritative and thorough, Histamine Receptors as Drug Targets seeks to aid pharmacologists, biochemists, drug discovery researchers, molecular biologists, chemists, toxicologists, lab scientists, medical doctors, principle investigators, research scientists, lab directors and technicians, as well as graduate students around the world in pursuing the study of this vital scientific area.
Histidine Phosphorylation: Methods and Protocols (Methods in Molecular Biology #2077)
by Claire E. EyersThis volume details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine. Chapters guide readers through in vitro systems, cell-based systems, comprehensive background review articles on histidine kinases and phosphatases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Histidine Phosphorylation: Methods and Protocols aims to ensure successful results in the further study of this rapidly growing field.
Histochemical and Cytochemical Methods of Visualization (Methods in Visualization)
by Jean-Marie ExbrayatHistochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualizatio
Histochemistry of Single Molecules
by Carlo Pellicciari Marco BiggiogeraThis detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets. It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.
Histochemistry of Single Molecules: Methods and Protocols (Methods in Molecular Biology #2566)
by Carlo Pellicciari Marco Biggiogera Manuela MalatestaThis volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.
Histology Protocols
by Ian A. Darby Tim D. HewitsonWith much of what we know about the pathogenesis of disease coming from the systematic and careful study of histological material, histology has proven to be an incredibly valuable area of study. In "Histology Protocols", expert researchers in the field contribute detailed methods involving the preparation of tissue, providing an overview of fixation, embedding, and processing, along with the required techniques for the retrieval of RNA from histological sections, followed by sections on routine and specialist histological staining techniques, including immuno-, lectin, and hybridization histochemistry, histological staining, as well as specific methods for the in situ identification of hypoxia and apoptosis, and details on the advances in imaging and image analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, "Histology Protocols" will provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques necessary to realize the potential of their resource.
Histology at a Glance (At A Glance Ser. #50)
by Michelle PeckhamThis brand new title provides a highly illustrated and unambiguous introduction to the science, structure and function of cells, their related pathology, and in particular, helps readers master the basic principles of recognizing features of histological sections. Histology at a Glance: Presents the perfect combination of text and image; key concepts are explained and superbly illustrated Provides coverage of all the main body systems and the components of the tissues of these systems Features practical information on microscopy, including sample preparation and magnification Includes hints and tips to help recognize features in a histological specimen, and overcome common difficulties Contains self-assessment questions to help test learning and recognition Links to the author's histological website, which features many more tips and samples, at www. wiley. com/go/histologyataglance Histology at a Glance is the perfect guide for medical, dentistry and biomedical science students, junior doctors, and is ideal for independent learning programmes in histology.
Histone Deacetylase Inhibitors in Combinatorial Anticancer Therapy
by Shabir Ahmad GanaiThis book reviews the latest developments in the design, synthesis, and molecular mechanism of action of Histone Deacetylase (HDAC) inhibitors in the context of potential cancer therapy. HDAC inhibitors are emerging as promising anticancer drug molecules that promote growth arrest, differentiation and apoptosis of cancer cells with tumor selective toxicity. The book begins with an overview of various epigenetic modifying enzymes that are involved in cancer transition and progression; before exploring the potential of HDACs in cancer treatment. It provides a classification of HDAC inhibitors based on their structural attributes, and addresses HDAC-induced cytotoxicity.. Lastly, it discusses and assesses the rationale behind therapies that combine HDAC inhibitors with other anticancer agents to treat solid tumors. Given its scope, it offers a valuable resource for all researchers, clinicians, and students working in formulation, drug discovery, oncology, and personalized medicine.
Histone Deacetylase Inhibitors — Epidrugs for Neurological Disorders
by Shabir Ahmad GanaiThis book provides an outline of epigenetics as a whole, while also specifically examining a range of epigenetic players, including histone acetyl transferases (HATs) and histone deacetylases (HDACs). It chiefly focuses on the emerging targets of HDACs and their implications for various neurological disorders, while also discussing the drawbacks of current therapeutic strategies, the classification of HDAC inhibitors, and their promising effects in connection with specific neurological disorders. The book explores the potential use of these inhibitors as novel therapeutic agents, considers the current challenges involved in using them to tackle neurological complications, and offers a novel solution by designing isoform-selective inhibitors and employing combinatorial therapeutic strategies. Its final section, which explores future directions, elaborates on the possibility of enhancing HDAC inhibitors’ therapeutic efficacy against various neurological complications.
Histone Deacetylases
by Sibaji SarkarThis volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors. "Histone Deacetylases: Methods and Protocols" is divided into four sections: Sections A and B describe methodologies used to detect the activity, function, or chromatin location of HDACs 1 through 11, with Section A discussing Class I and Section B discussing class II histone deacetylases; Section C focuses on the methodologies for cloning and characterizing the acetylation of SIRTs 1 through 7; and Section D describes methods related to histone deacetylase inhibitors. Written in the highly successful "Methods in Molecular Biology "series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, "Histone Deacetylases: Methods and Protocols, "is a valuable resource for investigators working on epigenetics, molecular biology, and genetics.
Histone Deacetylases: the Biology and Clinical Implication
by Edward Seto Tso-Pang YaoThe book highlights work from many different labs that taught us abnormal HDACs potentially contribute to the development or progression of many human diseases including immune dysfunctions, heart disease, cancer, memory impairment, aging, and metabolic disorders.
Histone Methyltransferases: Methods and Protocols (Methods in Molecular Biology #2529)
by Raphaël Margueron Daniel HolochThis volume provides methods used to investigate histone methyltransferase function. Chapters guide readers through a comprehensive set of approaches that detail phylogenetic diversity, histone demethylase activities in vitro, generating chromatin substrates, auto-methylation, quantification of metabolites, protein purification, crystallization, X-ray structure, cryogenic electron microscopy, assessing genome-wide patterns, CUT&Tag in mouse embryonic tissues, chemical biology approaches, peptide SPOT arrays, nascent chromatin capture, ectopic protein tethering, computational models, and development of methyltransferase inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Histone Methyltransferases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Histone Mutations and Cancer (Advances in Experimental Medicine and Biology #1283)
by Dong Fang Junhong HanThis book focuses on histone mutations, especially those mutations closely related to cancer. Genetic mutations and epigenetic alterations contribute to the development of a variety of cancers: recent genetic studies have identified e.g. H3K27M and H3G34R/V mutation in over 75% of DIPG cases, H3.3K36M mutation in more than 90% of chondroblastoma cases, and H3G34W/L mutation in over 90% of giant cell tumors of bone. Given the high incidence and tumorigenesis effects of histone H3 mutations, they are also referred to as oncohistones.This book highlights the advances made in the area over the past 10 years, and offers a state-of-the-art summary of epigenetic alternation, gene expression, protein structure, drug discovery, immunotherapy, and mouse modeling of histone H3 mutations in various tumors. Chiefly intended to provide researchers and graduate students with an overall picture of these mutations, it will also be of interest to researchers in basic oncology, clinical oncology, and epigenetics, as well as academics and clinical oncology practitioners.
Histone Recognition
by Ming-Ming ZhouThis book provides a timely review of the role of histone modifications in epigenetic control of gene expression. Topics covered include: basic mechanisms of molecular recognition of histone post-translational modification (PTMs); combinatorial readout of histone PTMs by tandem epigenome reader domains; genome-wide profiling of histone PTM interactions; small molecule modulation of histone PTM interactions and their potential as a new approach to therapeutic intervention in human diseases. All chapters were written by leading scientists who made the original key discoveries of the structure and mechanism of evolutionarily conserved reader domains, which serve to direct gene transcription in chromatin through interactions with DNA-packing histones in a PTM-sensitive manner.
Histone Variants: Methods and Protocols (Methods in Molecular Biology #1832)
by Guillermo A. Orsi Geneviève AlmouzniThis volume compiles methodologies used for detailed studies of histone variants, from their basic properties to their functional roles in chromatin and as vectors of epigenetic information. Its four sections cover experimental approaches to probe the biochemistry of histone variants and variant nucleosomes; their dynamics throughout the cell cycle and at specific genomic locations; their functional impact on chromosome organization and genome stability; and their importance in development, disease and evolution. Written in the highly successful Methods in Molecular Biology series format, chapters include lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, illustrations on the expected results, and tips on troubleshooting and avoiding known pitfalls. This anthology brings together contributions from many of the most highly recognized investigators in this community.Cutting-edge and thorough, Histone Variants: Methods and Protocols is a valuable resource for anyone interested in exploring the experimental and collaborative possibilities in this evolving field.
Histones
by Benoit Guillemette Luc R. GaudreauThis volume provides a comprehensive list of protocols for molecular biologists, biochemists and geneticists. Chapters cover protocols that further the study into protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Histones: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Histones and Other Basic Nuclear Proteins
by Gary S. Stein Janet L. SteinThis comprehensive book is a compilation of Professor Lubomir S. Hnilica's twenty years of research experimentally addressing the chemistry and the biological functions of chromosomal proteins. The histones and other nuclear proteins found associated with DNA in a number of tissues and cell types are featured. Lubomir Hnilica played a major role in establishing the extent to which these basic chromosomal polypeptides are conserved and the manner in which they interact with DNA to modify chromatin structure. In addition, non-histone chromosomal protein research is explained, and his technique of applying several biochemical and immunological approaches to the characterization of this complex and heterogeneous class of chromosomal polypeptides is discussed. Highlighted is the use of chemical crosslinking for studying protein/DNA interactions in intact cells. The proteins as well as the structure, organization, and regulation of the genes are also presented.
Histones: Methods and Protocols (Methods in Molecular Biology #2919)
by Benoit Guillemette Luc GaudreauThis second edition provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Histones: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.
Histopathology of Seed-Borne Infections
by Dalbir Singh S. B. MathurHistopathology of Seed-Borne Infections is the first book to provide comprehensive coverage of seed infection and disease. It includes an up-to-date account on the development and structure of seed, pointing out the structural variations in seeds of the plant families to which most crop plants belong. The text presents techniques and advice for his
Historia de la ciencia (Timelines of Science): Un recorrido visual a través del tiempo
by DKExplora el mundo de la ciencia como nunca antes en esta guía profusamente ilustrada.La historia de la humanidad ha avanzado gracias a los descubrimientos científicos. Desde que nuestros antepasados ??aprendieron a usar herramientas y fuego hasta los avances modernos que han dado forma al mundo en el que vivimos, la ciencia ha definido nuestra historia a lo largo de miles de años. Con bellas ilustraciones y textos claros y fáciles de leer, 'Historia de la ciencia' recorre la historia de la ciencia en todo el mundo y profundiza en la historia de las ideas, prácticas y progresos científicos. Con un enfoque visual y accesible único, este libro muestra como nunca antes cómo se originaron las ideas científicas clave y cómo han dado forma a nuestras vidas.Discover the world of science as never before in this richly illustrated guide.The history of humankind has been driven by scientific discovery. From our distant ancestors learning to use tools and fire for the first time, to the modern breakthroughs that have shaped the world we live in today, science has defined the story of humans for thousands of years. Using beautiful illustrations and clear, easy-to-read text, Timelines of Science explains the history of science as it unfolded around the world, and delves into the story of scientific ideas, practice, and progress one step at a time. Offering a uniquely accessible and visual approach, this book shows as never before where scientific ideas came from and how they have shaped all of our lives.
Historia del veneno: De la cicuta al polonio
by Adela Muñoz PáezCicuta, arsénico, cianuro, talio, polonio... Adela Muñoz realiza un insólito recorrido por los momentos más relevantes de la historia a partir de envenenamientos célebres La fascinación del ser humano por los venenos se remonta al principio de los tiempos. Ya en el antiguo Egipto y en el mundo grecorromano, los tósigos se convirtieron en un arma de poder. En Roma fueron muchos los emperadores que alcanzaron la gloria gracias a ellos: Nerón mató a su madre, Agripina la Menor, quien a su vez había asesina - do al emperador Claudio para conseguir la corona de su hijo. También en la Italia renacentista, y en la Francia de Luis XIV, las pócimas causaron estragos, y reyes, cortesanos y plebeyos perdieron la vida bajo sus efectos. Adela Muñoz nos descubre la composición de los venenos que acabaron con la vida de personajes ilustres como Sócrates, Cleopatra, el emperador Claudio, Rasputín o Adolf Hitler. Una fascinante historia desde la cicuta de la Antigüedad al polonio de nuestros días.
Historic Journeys Into Space (Images of America)
by Lynn M. Homan Thomas ReillyFrom the January 1958 launch of the first American satellite to the creation of the National Aeronautics and Space Administration by President Eisenhower just a few months later, from the heated space race of the Cold War era to the heroes of shuttle launches, the United States has been on the leading edge of space exploration and technology. Initially developed from one of Adolph Hitler's most feared weapons, the V2 rocket, the space program has accomplished much in just a few short decades. The first American manned space flight, which put astronaut Alan Shepard into space, was launched in 1961; in 1965, Edward White became the first American to walk in space--for a total of 21 minutes. In 1969, Neil Armstrong uttered those now-famous words as he became the first human to walk on the Moon. These and many other achievements, once considered unimaginable, have inspired and amazed the American people and engaged our brightest scientific minds.
Historical Biogeography of Neotropical Freshwater Fishes
by James S. Albert Roberto E. ReisThe fish faunas of continental South and Central America constitute one of the greatest concentrations of aquatic diversity on Earth, consisting of about 10 percent of all living vertebrate species. Historical Biogeography of Neotropical Freshwater Fishes explores the evolutionary origins of this unique ecosystem. The chapters address central themes in the study of tropical biodiversity: why is the Amazon basin home to so many distinct evolutionary lineages? What roles do ecological specialization, speciation, and extinction play in the formation of regional assemblages? How do dispersal barriers contribute to isolation and diversification? Focusing on whole faunas rather than individual taxonomic groups, this volume shows that the area's high regional diversity is not the result of recent diversification in lowland tropical rainforests. Rather, it is the product of species accumulating over tens of millions of years and across a continental arena.
Historical Climate Variability and Impacts in North America
by Cary J. Mock Lesley-Ann Dupigny-GirouxClimatologists with an eye on the past have any number of sources for their work, from personal diaries to weather station reports. Piecing together the trajectory of a weather event can thus be a painstaking process taking years and involving real detective work. Missing pieces of a climate puzzle can come from very far afield, often in unlikely places. In this book, a series of case studies examine specific regions across North America, using instrumental and documentary data from the 17th to the 19th centuries. Extreme weather events such as the Sitka hurricane of 1880 are recounted in detail, while the chapters also cover more widespread phenomena such as the collapse of the Low Country rice culture. The book also looks at the role of weather station histories in complementing the instrumental record, and sets out the methods that involve early instrumental and documentary climate data. Finally, the book's focus on North America reflects the fact that the historical climate community there has only grown relatively recently. Up to now, most such studies have focused on Europe and Asia. The four sections begin with regional case studies, and move on to reconstruct extreme events and parameters. This is followed by the role of station history and, lastly, methodologies and other analyses. The editors' aim has been to produce a volume that would be instrumental in molding the next generation of historical climatologists. They designed this book for use by general researchers as well as in upper-level undergraduate or graduate level courses.