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High-Throughput RNAi Screening
by David O. Azorsa Shilpi AroraHigh-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries. The success of these screening efforts is dependent on robust methodologies to perform these screens. In "High-Throughput RNAi Screening: Methods and Protocols, " expert researchers in the field share protocols and methods for performing high-throughput RNAi (HT-RNAi) screens. These include the use of various RNAi platforms and delivery methods in mammalian and non-mammalian systems, whole organism and cell models, and various applications, such as drug sensitizer identification. Finally, the book examines the latest advancements in the fields of assay development, library screening, data analysis, and hit selection. Written in the highly successful "Methods in Molecular Biology "series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, "High-Throughput RNAi Screening: Methods and Protocols "provides a comprehensive source of protocols and other necessary information to make robust and successful assays possible for all who wish to apply HT-RNAi in their research.
High Throughput Screening: The Discovery of Bioactive Substances
by John P. DevlinFurnishing the latest interdisciplinary information on the most important and frequently the only investigational system available for discovery programs that address the effects of small molecules on newly discovered enzyme and receptor targets emanating from molecular biology, this timely resource facilitates the transition from classical to high
High Throughput Screening
by William P. Janzen Paul BernasconiIn the years since the release of the popular first edition, the field of High Throughput Screening (HTS) has evolved considerably, from a small niche area of study to a major, essential scientific technique. In High Throughput Screening: Methods and Protocols, Second Edition, experts in the field fully update the previous edition to best serve as an introduction to HTS for scientists who are just entering the field, and as a provider of key, significant detail so as to be useful to scientists in established HTS operations. Featuring new screening technologies as well as many well established methods, chapters offer comprehensive treatment of the activities directly related to HTS, such as compound library management, data handling, and robotics. As a volume in the highly successful Methods in Molecular BiologyTM series, this work provides the kind of meticulous description and implementation advice that is crucial for getting optimal results. Authoritative and intrinsically practical, High Throughput Screening: Methods and Protocols, Second Edition promises to share the highest knowledge and enthusiasm with all those who use this versatile technology in their own labs.
High-Throughput Screening Assays in Toxicology
by Hao Zhu Menghang XiaThis book focuses on recently developed High Throughput Screening (HTS) assay protocols, many involved in the ToxCast and/or Tox21 initiatives, and the relevant HTS data analysis techniques. Divided into three sections, in vitro assays, in vivo assays, and computational techniques to analyze HTS data are all examined. Written for the highly successful "Methods in Molecular Biology" series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, "High Throughput Screening Assays in Toxicology" serves as a valuable reference resource for translating new HTS techniques into standardized chemical toxicity assessment tools in order to advance modern toxicology research to a new era where HTS techniques can partially replace the prevailing animal models.
High-Throughput Screening Assays in Toxicology (Methods in Molecular Biology #2474)
by Hao Zhu Menghang XiaThis second edition volume expands on the previous edition by exploring the latest advancements in high throughput screening (HTS) in toxicity studies by using in vitro, ex vivo, and in vivo models. This volume also covers the application of artificial intelligence (AI) and data science to curate, manage, and use HTS data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, High Throughput Screening Assays in Modern Toxicology, Second Edition is a valuable resource for scientists pursuing chemical toxicology research. This book will aid scientists and researchers in translating new HTS techniques into standardized chemical toxicology assessment tools that can refine, reduce, and replace animal testing.
High-Throughput Screening Methods in Toxicity Testing
by Pablo SteinbergExplores the benefits and limitations of the latest high-throughput screening methods With its expert coverage of high-throughput in vitro screening methods for toxicity testing, this book makes it possible for researchers to accelerate and streamline the evaluation and risk assessment of chemicals and drugs for toxicity. Moreover, it enables them to comply with the latest standards set forth by the U.S. National Research Council's "Toxicity Testing in the 21st Century: A Vision and Strategy" and the E.U.'s REACH legislation. Readers will discover a variety of state-of-the-science, high-throughput screening methods presented by a group of leading authorities in toxicology and toxicity testing. High-Throughput Screening Methods in Toxicity Testing is divided into five parts: General aspects, including predicting the toxicity potential of chemicals and drugs via high-throughput bioactivity profiling Assessing different cytotoxicity endpoints Assessing DNA damage and carcinogenesis Assessing reproductive toxicity, cardiotoxicity, and haematotoxicity Assessing drug metabolism and receptor-related toxicity Each chapter describes method principles and includes detailed information about data generation, data analysis, and applications in risk assessment. The authors not only enumerate the advantages of each high-throughput method over comparable conventional methods, but also point out the high-throughput method's limitations and potential pitfalls. In addition, the authors describe current research efforts to make high-throughput toxicity screening even more cost effective and streamlined. Throughout the book, readers will find plenty of figures and illustrations to help them understand and perform the latest high-throughput toxicity screening methods. This book is ideal for toxicologists and other researchers who need to implement high-throughput screening methods for toxicity testing in their laboratories as well as for researchers who need to evaluate the data generated by these methods.
High Tide at Gettysburg: The Campaign in Pennsylvania
by Glenn TuckerThe author tries to present how the Gettysburg battle was won and lost, and why the Gettysburg campaign remains such an appealing study to large numbers even after the passing of nearly a century.
High Tide for Horseshoe Crabs
by Lisa Kahn SchnellDual-layered text introduces the life cycle of the horseshoe crab, with a focus on the annual mass-spawning event at Delaware Bay.
High Time-Resolution Astrophysics (Canary Islands Winter School of Astrophysics #27)
by Tariq Shahbaz Jorge Casares Velázquez Teodoro Muñoz DariasHigh time-resolution astrophysics (HTRA) involves measuring and studying astronomical phenomena on timescales of seconds to milliseconds. Although many areas of astronomy, such as X-ray astronomy and pulsar observations, have traditionally required high time-resolution studies, HTRA techniques are now being applied to optical, infrared and gamma-ray wavelength regimes, due to the development of high efficiency detectors and larger telescopes that can gather photons at a higher rate. With lectures from eminent scientists aimed at young researchers and postdoctorate students in observational astronomy and astrophysics, this volume gives a practical overview and introduction to the tools and techniques of HTRA. Just as multi-spectral observations of astrophysical phenomena are already yielding new scientific results, many astronomers are optimistic that exploring the time domain will open up an important new frontier in observational astronomy over the next decade.
High-Vacuum Technology: A Practical Guide, Second Edition
by Marsbed H. HablanianOffering a basic understanding of each important topic in vacuum science and technology, this book concentrates on pumping issues, emphasizes the behavior of vacuum pumps and vacuum systems, and explains the relationships between pumps, instrumentation and high-vacuum system performance. The book delineates the technical and theoretical aspects of the subject without getting in too deep. It leads readers through the subtleties of vacuum technology without using a dissertation on mathematics to get them there. An interesting blend of easy-to-understand technician-level information combined with engineering data and formulae, the book provides a non-analytical introduction to high vacuum technology.
High Value Fermentation Products, Volume 1: Human Health
by Saurabh Saran Vikash Babu Asha ChaubeyGreen technologies are no longer the “future” of science, but the present. With more and more mature industries, such as the process industries, making large strides seemingly every single day, and more consumers demanding products created from green technologies, it is essential for any business in any industry to be familiar with the latest processes and technologies. It is all part of a global effort to “go greener,” and this is nowhere more apparent than in fermentation technology. This book describes relevant aspects of industrial-scale fermentation, an expanding area of activity, which already generates commercial values of over one third of a trillion US dollars annually, and which will most likely radically change the way we produce chemicals in the long-term future. From biofuels and bulk amino acids to monoclonal antibodies and stem cells, they all rely on mass suspension cultivation of cells in stirred bioreactors, which is the most widely used and versatile way to produce. Today, a wide array of cells can be cultivated in this way, and for most of them genetic engineering tools are also available. Examples of products, operating procedures, engineering and design aspects, economic drivers and cost, and regulatory issues are addressed. In addition, there will be a discussion of how we got to where we are today, and of the real world in industrial fermentation. This chapter is exclusively dedicated to large-scale production used in industrial settings.
High Voltage Direct Current Transmission
by Khaled Ahmed Dragan JovcicThis comprehensive reference guides the reader through all HVDC technologies, including LCC (Line Commutated Converter), 2-level VSC and VSC HVDC based on modular multilevel converters (MMC) for an in-depth understanding of converters, system level design, operating principles and modeling. Written in a tutorial style, the book also describes the key principles of design, control, protection and operation of DC transmission grids, which will be substantially different from the practice with AC transmission grids.The first dedicated reference to the latest HVDC technologies and DC grid developments; this is an essential resource for graduate students and researchers as well as engineers and professionals working on the design, modeling and operation of DC grids and HVDC. Key features: * Provides comprehensive coverage of LCC, VSC and (half and full bridge) MMC-based VSC technologies and DC transmission grids. * Presents phasor and dynamic analytical models for each HVDC technology and DC grids. * Includes HVDC protection, studies of DC and AC faults, as well as system-level studies of AC-DC interactions and impact on AC grids for each HVDC technology. * Companion website hosts SIMULINK SimPowerSystems models with examples for all HVDC topologies.
High Voltage Measurement Techniques: Fundamentals, Measuring Instruments, and Measuring Methods (Power Systems)
by Klaus SchonThis book conveys the theoretical and experimental basics of a well-founded measurement technique in the areas of high DC, AC and surge voltages as well as the corresponding high currents. Additional chapters explain the acquisition of partial discharges and the electrical measured variables. Equipment exposed to very high voltages and currents is used for the transmission and distribution of electrical energy. They are therefore tested for reliability before commissioning using standardized and future test and measurement procedures. Therefore, the book also covers procedures for calibrating measurement systems and determining measurement uncertainties, and the current state of measurement technology with electro-optical and magneto-optical sensors is discussed.
High-Voltage Test and Measuring Techniques
by Eberhard Lemke Wolfgang HauschildThe new edition of this book incorporates the recent remarkable changes in electric power generation, transmission and distribution. The consequences of the latest development to High Voltage (HV) test and measuring techniques result in new chapters on Partial Discharge measurements, Measurements of Dielectric Properties, and some new thoughts on the Shannon Theorem and Impuls current measurements. This standard reference of the international high-voltage community combines high voltage engineering with HV testing techniques and HV measuring methods. Based on long-term experience gained by the authors the book reflects the state of the art as well as the future trends in testing and diagnostics of HV equipment. It ensures a reliable generation, transmission and distribution of electrical energy. The book is intended not only for experts but also for students in electrical engineering and high-voltage engineering.
The Highbush Blueberry and Its Management
by Robert E GoughHere is a book that sets forth vital information growers need to produce highbush blueberries effectively and efficiently. Written from the grower?s point of view, The Highbush Blueberry and Its Management presents technical information in a highly readable manner that is easy to understand. It helps growers make proper decisions before they plant-
Higher and Colder: A History of Extreme Physiology and Exploration
by Vanessa HeggieDuring the long twentieth century, explorers went in unprecedented numbers to the hottest, coldest, and highest points on the globe. Taking us from the Himalaya to Antarctica and beyond, Higher and Colder presents the first history of extreme physiology, the study of the human body at its physical limits. Each chapter explores a seminal question in the history of science, while also showing how the apparently exotic locations and experiments contributed to broader political and social shifts in twentieth-century scientific thinking. Unlike most books on modern biomedicine, Higher and Colder focuses on fieldwork, expeditions, and exploration, and in doing so provides a welcome alternative to laboratory-dominated accounts of the history of modern life sciences. Though centered on male-dominated practices—science and exploration—it recovers the stories of women’s contributions that were sometimes accidentally, and sometimes deliberately, erased. Engaging and provocative, this book is a history of the scientists and physiologists who face challenges that are physically demanding, frequently dangerous, and sometimes fatal, in the interest of advancing modern science and pushing the boundaries of human ability.
Higher Biology: Practice Papers for SQA Exams
by Graham Moffat Billy DicksonPractise for your SQA exams with three specially-commissioned Hodder Gibson Practice Exam Papers.- Practise with model papers written and checked by experienced markers and examiners- Get extra advice with specially-written study-skills guidance sections- Gain vital extra marks and avoid common mistakes with examiner tips
Higher Biology for CfE: Multiple Choice and Matching
by James Simms Clare Marsh Caroline StevensonThe perfect practice book for multiple choice and matching exercises that make up 20% of the final CfE Higher exam paper. This book offers ideal material for either classroom or homework activities, and allows students to construct a glossary of terms essential to the course, while banks of multiple choice questions enable them to practise extensivelty for the examination.
Higher Biology, Second Edition
by Clare Marsh James Simms Caroline Stevenson James Torrance James FullartonExam Board: SQALevel: HigherSubject: BiologyFirst Teaching: August 2018First Exam: June 2019Ensure that students are prepared for every aspect of Higher Biology with the new edition of this popular textbook from James Torrance and his renowned author team, completely updated for the 2018 changes to the SQA Higher Biology syllabus.- Suggested learning activities throughout help to develop students' knowledge and skills including all new case studies, research topics and investigations- Testing your knowledge questions at the end of each chapter provide opportunities to continually assess Knowledge and Understanding, and are particularly useful for homework tasks- 'What you should know' summaries of key facts and concepts provide an excellent source of material for consolidation and revision prior to the SQA examination.- 'Applying Your Knowledge and Skills' sections at the end of each section have been substantially extended to give students extra practice in exam questions and foster the development of Skills of Scientific Experimentation, Investigation and Enquiry
Higher Biology, Second Edition
by Clare Marsh James Simms Caroline Stevenson James Torrance James FullartonExam Board: SQALevel: HigherSubject: BiologyFirst Teaching: August 2018First Exam: June 2019Ensure that students are prepared for every aspect of Higher Biology with the new edition of this popular textbook from James Torrance and his renowned author team, completely updated for the 2018 changes to the SQA Higher Biology syllabus.- Suggested learning activities throughout help to develop students' knowledge and skills including all new case studies, research topics and investigations- Testing your knowledge questions at the end of each chapter provide opportunities to continually assess Knowledge and Understanding, and are particularly useful for homework tasks- 'What you should know' summaries of key facts and concepts provide an excellent source of material for consolidation and revision prior to the SQA examination.- 'Applying Your Knowledge and Skills' sections at the end of each section have been substantially extended to give students extra practice in exam questions and foster the development of Skills of Scientific Experimentation, Investigation and Enquiry
Higher Biology: Second Edition
by James Simms Clare Marsh Caroline StevensonExam Board: SQALevel: HigherSubject: BiologyFirst Teaching: August 2018First Exam: June 2019Ensure that students are prepared for every aspect of Higher Biology with the new edition of this popular textbook from James Torrance and his renowned author team, completely updated for the 2018 changes to the SQA Higher Biology syllabus.- Suggested learning activities throughout help to develop students' knowledge and skills including all new case studies, research topics and investigations- Testing your knowledge questions at the end of each chapter provide opportunities to continually assess Knowledge and Understanding, and are particularly useful for homework tasks- 'What you should know' summaries of key facts and concepts provide an excellent source of material for consolidation and revision prior to the SQA examination.- 'Applying Your Knowledge and Skills' sections at the end of each section have been substantially extended to give students extra practice in exam questions and foster the development of Skills of Scientific Experimentation, Investigation and Enquiry
Higher Chemistry: Second Edition Epub
by John AndersonExam Board: SQALevel: HigherSubject: ChemistryFirst Teaching: August 2018First Exam: June 2019Full course coverage in this new Higher Chemistry textbook, updated for the latest changes to the SQA coursework and question papers.- In-text, Study and End-of-course questions have been updated and extended in this edition, testing students' knowledge and understanding of the chemistry presented and offering lots of practice to revise and consolidate ahead of the exam.- Worked examples show common Higher Chemistry questions and ways of answering that cover the necessary points- Checklists for Revision provide short summaries of the key learning points at the end of each chapter so that students can this to self-check their learning which helps them revise for assessments- Brand new section 'Additional features of the Higher Chemistry exam' offers advice on how to tackle two important new features of the exam: Numeracy and Open-ended questions- Key terms and Chemical Dictionary aid understanding and allow students to check their knowledge of the key terms
Higher Chemistry: Revision Notes and Questions
by David Calder John AndersonThe perfect revision companion to the Higher Chemistry textbook, this newly written resource reflects the very latest SQA curriculum.This book offers bite-sized facts, worked examples and a variety of question types to support and reinforce learning and practice for the final examination.- Dedicated sections on numeracy and researching chemistry- Provides a broad range of questions, including open-ended and past SQA exam questions- Answers section allows students to check their understanding of the standards required for Higher level success
Higher Chemistry: Practice Papers for SQA Exams
by Barry McbridePractise for your SQA exams with three specially-commissioned Hodder Gibson Practice Exam Papers.- Practise with model papers written and checked by experienced markers and examiners- Get extra advice with specially-written study-skills guidance sections- Gain vital extra marks and avoid common mistakes with examiner tips
Higher Chemistry, Second Edition
by John AndersonExam Board: SQALevel: HigherSubject: ChemistryFirst Teaching: August 2018First Exam: June 2019Full course coverage in this new Higher Chemistry textbook, updated for the latest changes to the SQA coursework and question papers.- In-text, Study and End-of-course questions have been updated and extended in this edition, testing students' knowledge and understanding of the chemistry presented and offering lots of practice to revise and consolidate ahead of the exam.- Worked examples show common Higher Chemistry questions and ways of answering that cover the necessary points- Checklists for Revision provide short summaries of the key learning points at the end of each chapter so that students can this to self-check their learning which helps them revise for assessments- Brand new section 'Additional features of the Higher Chemistry exam' offers advice on how to tackle two important new features of the exam: Numeracy and Open-ended questions- Key terms and Chemical Dictionary aid understanding and allow students to check their knowledge of the key terms