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Plant-Microbe Interaction and Stress Management (Rhizosphere Biology)
by Puneet Singh Chauhan Shri Krishna Tewari Sankalp MisraThis book provides a comprehensive view for plant microbe interactions towards stress management and microbiome-assisted approaches in sustainable agriculture. It is divided into four major sections. The book gives insights into the increasing threat of abiotic and biotic stresses and the accompanying challenges to modern agriculture. Through different chapters, the book shows how various microorganisms could ameliorate abiotic and biotic stress, and contribute towards food sustainability and restore ecosystem functioning. It provides a deep understanding of soil microbiome and its interaction with plants, to enhance food security. It further talks about metagenomic approaches for methodological tool for studying the soil microbiome. Separate sections on stress, talk at length about the various abiotic and biotic stresses that plants are faced it. The book culminates with an exciting section on microbiome-assisted approaches for combating stress. It talks about the different microbiomes such as rhizosphere, soil, phyllosphere and endophytic microbiome. The book would be beneficial to students, researchers and course instructors in microbiology, botany, plant pathology and agriculture.
Plant-Microbe Interactions: Laboratory Techniques (Springer Protocols Handbooks)
by N. Amaresan M. Senthilkumar A SankaranarayananThis manual details the techniques involved in the study of plant microbe interactions (PMI). Covering a wide range of basic and advanced techniques associated with research on biological nitrogen fixation, microbe-mediated plant nutrient use efficiency, the biological control of plant diseases and pests such as nematodes, it will appeal to postgraduate students, research scholars and postdoctoral fellows, as well as teachers from various fields, including pathology, entomology and agronomy.It consists of five broad sections featuring different units. Information panels at the beginning of each unit present essential knowledge as well as advances in a particular topic. The manual can also serve as a textbook for undergraduate courses like Techniques for Plant-Microbe Interactions; Biological Control of Plant Diseases; and Nutrient Use Efficiency. Providing basic insights and working protocols from all related disciplines, this unique laboratory manual is a valuable resource for researchers interested in investigating PMI.
Plant-Microbe Interactions: Harnessing Next-Generation Molecular Technologies for Sustainable Agriculture (Advances and Applications in Biotechnology)
by Jagajjit SahuA constant research effort to understand plant-microbe interactions makes it indispensable to keep abreast of the latest research developments. Researchers from a range of disciplines have used multiple approaches to infer this field. With the advent of next-generation techniques, both molecular and computational, the field has entered a new phase. These approaches often result in massive information, which is sometimes tangled and in need of further analysis. These types of analyses also require cutting-edge data analytics as well as efficient statistical models. Plant-Microbe Interactions: Harnessing Next-Generation Molecular Technologies for Sustainable Agriculture provides a comprehensive picture of the modern-day analytics and approaches being used to provide insights into the interactions between plant and microbe. A wide range of technologies are explored along with practical guides toward these techniques. A detailed understanding of omics data in various areas could be obtained from this compilation. Key Features: • Crosstalk between plant and microbe • Overview of advanced molecular techniques used to study plant-microbe interaction • Practical guide to technologies such as NGS • Omics data analysis used to study plant-microbe interaction • Role of soil metagenomics • Advanced technologies such as nanotechnology and CRISPR serving to study plant-microbe interaction This book will serve as a great reference to various next-generation techniques in the field of plant-microbe interaction, thereby helping to better understand the mechanism. This will also help budding researchers to shape their research in similar areas.
Plant-Microbe Interactions in Agro-Ecological Perspectives
by Dhananjaya Pratap Singh Harikesh Bahadur Singh Ratna PrabhaThis books presents an updated compilation on fundamental interaction mechanisms of microbial communities with the plant roots and rhizosphere (belowground) and leaves and aerial parts (aboveground). Plant rhizopshere recruits its own microbial composition that survive there and help plants grow and develop better under biotic and abiotic conditions. Similar is the case with the beneficial microorganisms which are applied as inoculants with characteristic functions. The mechanism of plant-microbe interactions is interesting phenomenon in biological perspectives with numerous implications in the fields. The First volume focuses on the basic and fundamental mechanisms that have been worked out by the scientific communities taking into account different plant-microbe systems. This includes methods that decipher mechanisms at cellular, physiological, biochemical and molecular levels and the functions that are the final outcome of any beneficial or non-beneficial interactions in crop plants and microbes. Recent advances in this research area is covered in different book chapters that reflect the impact of microbial interactions on soil and plant health, dynamics of rhizosphere microbial communities, interaction mechanisms of microbes with multiple functional attributes, microbiome of contrasting crop production systems (organic vs conventional), mechanisms behind symbiotic and pathogenic interactions, endophytic (bacterial and fungal) interaction and benefits, rhizoplane and endosphere associations, signalling cascades and determinants in rhizosphere, quorum sensing in bacteria and impact on interaction, mycorrhizal interaction mechanisms, induced disease resistance and plant immunization, interaction mechanisms that suppress disease and belowground microbial crosstalk with plant rhizosphere. Methods based on multiphasic and multi-omics approaches were discussed in detail by the authors. Content-wise, the book offers an advanced account on various aspects of plant-microbe interactions and valuable implications in agro-ecological perspectives.
Plant Microbe Interface
by Ajit Varma Swati Tripathi Ram PrasadThis book shares the latest insights into the genetic basis of molecular communication between plants and their microbial consortia. Further, the book highlights the capabilities of the rhizosphere and endosphere, which help manage ecosystem responses to climate change, nutrient cycling and sequestration of carbon; and discusses their application to the development and management of renewable energy sources.In their natural environments, plants are surrounded by a tremendous number of microorganisms. Some microbes directly interact with plants in a mutually beneficial fashion, while others colonize plants solely for their own advantage. In addition, microbes can indirectly affect plants by drastically altering their environments. Understanding the complex nature of the plant-microbe interface (PMI) can pave the way for novel strategies to improve plant productivity in an eco-friendly manner.The PMI approach focuses on understanding the physical, molecular, and chemical interactions between organisms in order to determine their functional roles in biological, physical, chemical and environmental systems. Although several metabolites from plants and microbes have now been fully characterized, their roles in chemical interactions between these associates remain poorly understood, and require further investigation.
Plant Microbe Symbiosis
by Ajit Varma Ram Prasad Swati TripathiThis book provides an overview of the latest advances concerning symbiotic relationships between plants and microbes, and their applications in plant productivity and agricultural sustainability. Symbiosis is a living phenomenon including dynamic variations in the genome, metabolism and signaling network, and adopting a multidirectional perspective on their interactions is required when studying symbiotic organisms. Although various plant-microbe symbiotic systems are covered in this book, it especially focuses on arbuscular mycorrhiza (AM) symbiosis and root nodule symbiosis, the two most prevalent systems. AM symbiosis involves the most extensive interaction between plants and microbes, in the context of phylogeny and ecology. As more than 90% of all known species of plants have the potential to form mycorrhizal associations, the productivity and species composition, as well as the diversity of natural ecosystems, are frequently dependent upon the presence and activity of mycorrhizas. In turn, root nodule symbiosis includes morphogenesis and is formed by communication between plants and nitrogen-fixing bacteria. The biotechnological application of plant–microbe symbiosis is expected to foster the production of agricultural and horticultural products while maintaining ecologically and economically sustainable production systems. Designed as a hands-on guide, this book offers an essential resource for researchers and students in the areas of agri-biotechnology, soil biology and fungal biology.
Plant Microbe Symbiosis- Fundamentals and Advances
by Naveen Kumar AroraPlant microbe interaction is a complex relationship that can have various beneficial impacts on both the communities. An urgent need of today's world is to get high crop yields in an ecofriendly manner. Utilization of beneficial and multifaceted plant growth promoting (PGP) microorganisms can solve the problem of getting enhanced yields without disturbing the ecosystem thus leading to sustainability. For this to achieve understanding of the intricate details of how the beneficial microbes form associations with the host plant and sustain that for millions of years must be known. A holistic approach is required wherein the diversity of microbes associated with plant and the network of mechanisms by which they benefit the host must be studied and utilized. 'Plant Microbe Symbiosis - Fundamentals and Advances' provides a comprehensive understanding of positive interactions that occur between plant and microorganisms and their utilization in the fields. The book reviews the enormous diversity of plant associated microbes, the dialog between plant-microbes-microbes and mechanisms of action of PGP microbes. Utilization of PGPRs as nutrient providers, in combating phytopathogens and ameliorating the stressed and polluted soils is also explained. Importantly, the book also throws light on the unanswered questions and future direction of research in the field. It illustrates how the basic knowledge can be amalgamated with advanced technology to design the future bioformulations.
Plant-Microbes-Engineered Nano-particles: Understanding the Interaction of Plant, Microbes and Engineered Nano-particles (ENPS) (Advances in Science, Technology & Innovation)
by Pardeep Singh Rishikesh Singh Pramit Verma Rahul Bhadouria Ajay Kumar Mahima KaushikThis book presents a collection of cross-disciplinary research, with contributions addressing all key features of the plant/microbe/ENP nexus in agro-ecosystems. The uptake, transport and transformation of nanoparticles in plants have attracted more and more attention in the past several years. Especially, the impact of Engineered Nanoparticles (ENPs) on bioprocesses; low-, medium- and high-level dose responses in the microbial community of soil; and long-, medium- and short-term exposure responses, particularly microbial nitrogen transformations, are just a few of the aspects involved. Since ENPs are used in many industries, including cosmetics, agriculture, medicine, food technology and waste management, their transport through biogeochemical cycles is an important focus of many studies today.Specifically, ENP–microbe interaction has been analysed with regard to disease treatment for plants; it plays a vital role in disease inhibition by releasing metal ions that act through many pathways – e.g. reactive oxygen species (ROS) generation, DNA transformation and disruption of the cell cycle – to stop cell growth in the pathogen. Due to these properties, ENPs are also used as slow release or delayed release pesticides and fungicides, and as carrier systems for growth-promoting hormones. Despite their multiple uses in various industries, the negative effects of ENPs are still a major concern for the scientific community and consumers alike. For example, their transport to various food chains has been reported to have adverse effects. This raises a degree of doubt concerning a rapidly growing scientific field with major applications in many industries.From a sustainable development perspective and particularly to ensure food security in light of the uncertainty accompanying climate change, it is imperative to address this divergence by focusing on the plant/microbe/ENP nexus.
Plant Microbes Symbiosis: Applied Facets
by Naveen Kumar AroraPlants form mutualistic association with various microorganisms, particularly in the rhizosphere region. The association benefits both the partners in a number of ways. A single plant can support the growth of diverse microbes and in reciprocation these microbes help the plant in several ways. A great deal of knowledge is now available on the mechanisms of action of plant growth promoting microbes in forming association with their partner plant and benefitting it. With ever increasing population and to achieve food security it has become utmost necessary to utilize these friendly microbes to enhance the crop yield and quality in an ecofriendly and sustainable manner. We already know about the huge negative impact of chemicals used in agriculture on the humans and the ecosystems as whole. 'Plant Microbes Symbiosis - Applied Facets' provides a comprehensive knowledge on practical, functional and purposeful utility of plant-microbe interactions. The book reviews the utilization of beneficial microbes for crop yield enhancement and protection against diseases caused by phytopathogens and nutrient deficiencies. The tome also reviews the utility of plant growth promoting microbes in helping the plants to deal with abiotic stresses imposed by climate change and anthropogenic activities. The book showcases how plant-microbe interactions are or can be utilized for reclamation of stressed soils and degradation of pollutants in a most effective and environment friendly manner. It also ascertains the reasons for the below par performance of the microbial based inoculants. The utilization of biotechnological tools for development of next generation bioformulations to combat the new challenges and overcome past hurdles has been discussed. This wonderful association between plants and microbes if used properly will not only enhance the crop yields and reclaim barren lands but also make our planet a better place to live on for all of its habitants.
Plant-Microbial Interactions and Smart Agricultural Biotechnology (Microbial Biotechnology for Food, Health, and the Environment)
by Swati Tyagi, Robin Kumar, Baljeet Singh Saharan, and Ashok Kumar NaddaConsidering the ever-increasing global population and finite arable land, technology and sustainable agricultural practices are required to improve crop yield. This book examines the interaction between plants and microbes and considers the use of advanced techniques such as genetic engineering, revolutionary gene editing technologies, and their applications to understand how plants and microbes help or harm each other at the molecular level. Understanding plant-microbe interactions and related gene editing technologies will provide new possibilities for sustainable agriculture. The book will be extremely useful for researchers working in the fields of plant science, molecular plant biology, plant-microbe interactions, plant engineering technology, agricultural microbiology, and related fields. It will be useful for upper-level students and instructors specifically in the field of biotechnology, microbiology, biochemistry, and agricultural science. Features: Examines the most advanced approaches for genetic engineering of agriculture (CRISPR, TALAN, ZFN, etc.). Discusses the microbiological control of various plant diseases. Explores future perspectives for research in microbiological plant science. Plant-Microbial Interactions and Smart Agricultural Biotechnology will serve as a useful source of cutting-edge information for researchers and innovative professionals, as well as upper-level undergraduate and graduate students taking related agriculture and environmental science courses.
Plant Microbiology
by Andrew Holmes Michael GillingsPlant Microbiology provides a comprehensive source of information on DNA sequencing and mapping, the newest technology and procedures in areas such as radiation hybrid mapping, FISH and specialized sequencing techniques are covered. The book also describes how transgene expression is controlled in plants and how advanced information strategies can be used to manipulate and modify the plant genome. An exciting final chapter provides and overview of all the applications of plant transformation in agriculture, medicine and industry.
The Plant Microbiome: Methods and Protocols (Methods in Molecular Biology #2232)
by Lilia C. Carvalhais Paul G. DennisThis volume provides methods, protocols, and reviews that are useful for new and experienced plant microbiome researchers. Chapters guide readers through the investigation of microbiomes associated with seeds, sampling microbiomes from plant compartments and tissues, culture-based methods, culture-independent metabarcoding methods, methods to obtain DNA and perform metabarcoding, protocols to block PCR amplification from the plant host, qPCR-based methods, editing of specific genes in Bacillus genomes, and Streptomycetes and plant microbial indicators. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Plant Microbiome: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Plant Microbiome: Stress Response
by Dilfuza Egamberdieva Parvaiz AhmadThis book presents state-of-the-art research on the many facets of the plant microbiome, including diversity, ecology, physiology and genomics, as well as molecular mechanisms of plant-microbe interactions. Topics considered include the importance of microbial secondary metabolites in stimulating plant growth, induced systemic resistance, tolerance to abiotic stress, and biological control of plant pathogens. The respective contributions show how microbes help plants to cope with abiotic stresses, and represent significant progress toward understanding the complex regulatory networks critical to host-microbe interaction and plant adaptation in extreme environments. New insights into the mechanisms of microbial actions in inducing plant stress tolerance open new doors for improving the efficacy of microbial strategies, and could produce new ways of economically increasing crop yields without harming the environment. As such, this book offers an essential resource for students and researchers with an interest in plant-microbe interaction, as well as several possibilities for employing the plant microbiome in the enhancement of crop productivity under future climate change scenarios.
Plant Microbiome and Biological Control: Emerging trends and applications (Sustainability in Plant and Crop Protection #20)
by Piyush Mathur Swarnendu RoyThis book offers a comprehensive guide to discovering, assessing, and utilizing consortia of beneficial microbes for crop protection and enhanced crop production in the context of climate change. It provides deep insights into the functional roles of the rhizomicrobiome, including AMF, endophytes, PGPRs, and the phyllomicrobiome, as well as the microbiomes of different plant parts such as seeds, fruits, and stems, in promoting plant growth, development, and the biocontrol of pests and pathogens in a sustainable manner. The book also presents the latest updates on molecular biology techniques, genetic engineering, biotechnological tools, and metagenomics, which are widely used for analyzing plant-pathogen interactions and microbial identification. It will be especially valuable for students and faculty involved in the study and teaching of plant–microbe interactions, as well as researchers working on sustainable methods for plant disease management. With cutting-edge research from leading experts, this book aims to contribute to the development of an eco-friendly, sustainable agricultural system.
Plant Microbiome Engineering (Methods and Protocols in Food Science)
by Dhanasekaran Dharumadurai A. Sankara NarayananThis volume provides lab-oriented protocols to deal the various plant microbiome engineering approaches in a lucid and simple manner. Chapters are divided into four section detailing plant associated microbiomes, single cell genomics, whole community metagenomics, metabolic network monitoring and advanced methods in plant microbiome engineering. Written in the format of the Methods and Protocols in Food Science series, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well-established and validated methods for readily reproducible laboratory protocols and contain notes on how to avoid or solve typical problems. Authoritative and cutting-edge, Plant Microbiome Engineering aims to provide well-established protocols and procedures largely used by both academics and industrials.
Plant Microbiome for Plant Productivity and Sustainable Agriculture (Microorganisms for Sustainability #37)
by Sagar Chhabra Ram Prasad Naga Raju Maddela Narendra TutejaThis edited book deals with latest comprehensive information on conventional and high throughput techniques and technologies that are recently used to study plant microbial interface for agricultural research and enhancing plant productivity. Plant microbiota are important for many plant growth promotion activity and agricultural productivity and are sustainable green technology for enhancing agricultural productivity under changing environment. The book covers recent information about the plant associated microbiota and their ecology. It discusses technologies to isolate and test microbiota inhabiting in different portion of plants. The book explores the conventional methods as well as the most recently recognized high throughput technologies which are important for productive agroecosystems to feed the growing global population.This book is of interest to teachers, researchers, microbiologist, plant and environmental scientist and those interested in environment stewardship around the world. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences and policy makers to be a useful to read.
The Plant Microbiome in Sustainable Agriculture
by Prem Lal Kashyap Alok Kumar Srivastava Madhumita SrivastavaTranslational Microbiome for Sustainable Agriculture will invite global experts and distinguished investigators conducting phytomicrobiome research and develop a comprehensive reference book with up-to-date information regarding the microbiome studies and trends in the field of agricultural sustainability. It covers both terrestrial and crop associated microbiomes, unveiling biological, biotechnological and technical aspects of research. This book will be devoted to students and professionals interested in learning techniques for microbiome surveys, including culture-independent approaches, and to better understand the biology of microorganisms in nature and commercializing of microbiome derived new products, with major emphasis on sustainable agriculture under changing environment scenario. Using this information as a basis, a non-specialist reader should be able to understand more complex articles and to discuss selected topics with colleagues.
Plant Microbiome Paradigm
by Ajit Varma Swati Tripathi Ram PrasadThis book provides a comprehensive overview of the current state of knowledge on plant-microbiome interactions and associations. It covers all major mechanistic approaches used to investigate microbes’ impacts on plant growth promotion, disease control and health.The industrial manufacture of nitrogen currently accounts for roughly 2% of the world’s total energy consumption. Microbial products are expected to reduce the need for costly fertilizers, as well as chemical pesticides and fungicides. While beneficial microorganisms are increasingly being used in agriculture, abiotic and biotic stresses such as heat, drought, cold, and salt can quickly kill or render them useless in the field. However, discovering new and better treatments is a lengthy process due to the considerable microbial diversity found in soils.Researchers have now proposed using biotechnological approaches to accelerate the process of microbial technology development. The fact that plant-associated microbes stimulate plant growth and development is well known, as the examples of rhizobia and mycorrhizal fungi show. The mechanisms by which these microorganisms maintain plant growth include the production of phytohormones, fixation of nitrogen, and the mobilization of phosphorus and minerals. The plant microbiome is also involved in pathogen suppression, and especially the root microbiome acts as a protective shield against soil-borne pathogens.A special feature of this book is its multidisciplinary approach, spanning from plant microbiology/biocontrol, fungal and bacterial endophytes, plant physiology, to biochemistry, proteomics and genomics. It is ideally suited for researchers and student of agri-biotechnology, soil biology and fungal biology.
Plant Microbiomes for Sustainable Agriculture (Sustainable Development and Biodiversity #25)
by Joginder Singh Ajar Nath Yadav Ali Asghar Rastegari Neelam YadavThis book encompasses the current knowledge of plant microbiomes and their potential biotechnological application for plant growth, crop yield and soil health for sustainable agriculture. The plant microbiomes (rhizospheric, endophytic and epiphytic) play an important role in plant growth, development, and soil health. Plant and rhizospheric soil are a valuable natural resource harbouring hotspots of microbes, and it plays critical roles in the maintenance of global nutrient balance and ecosystem function. The diverse group of microbes is key components of soil–plant systems, where they are engaged in an intense network of interactions in the rhizosphere/endophytic/phyllospheric. The rhizospheric microbial diversity present in rhizospheric zones has a sufficient amount of nutrients release by plant root systems in form of root exudates for growth, development and activities of microbes. The endophytic microbes are referred to those microorganisms, which colonize in the interior of the plant parts, viz root, stem or seeds without causing any harmful effect on host plant. Endophytic microbes enter in host plants mainly through wounds, naturally occurring as a result of plant growth, or through root hairs and at epidermal conjunctions. Endophytes may be transmitted either vertically (directly from parent to offspring) or horizontally (among individuals). The phyllosphere is a common niche for synergism between microbes and plant. The leaf surface has been termed as phyllosphere and zone of leaves inhabited by microorganisms as phyllosphere. The plant part, especially leaves, is exposed to dust and air currents resulting in the establishments of typical flora on their surface aided by the cuticles, waxes and appendages, which help in the anchorage of microorganisms. The phyllospheric microbes may survive or proliferate on leaves depending on extent of influences of material in leaf diffuseness or exudates. The leaf diffuseness contains the principal nutrients factors (amino acids, glucose, fructose and sucrose), and such specialized habitats may provide niche for nitrogen fixation and secretions of substances capable of promoting the growth of plants. The microbes associated with plant as rhizospheric, endophytic and epiphytic with plant growth promoting (PGP) attributes have emerged as an important and promising tool for sustainable agriculture. PGP microbes promote plant growth directly or indirectly, either by releasing plant growth regulators; solubilization of phosphorus, potassium and zinc; biological nitrogen fixation or by producing siderophore, ammonia, HCN and other secondary metabolites which are antagonistic against pathogenic microbes. The PGP microbes belong to different phylum of archaea (Euryarchaeota); bacteria (Acidobacteria, Actinobacteria, Bacteroidetes, Deinococcus-Thermus, Firmicutes and Proteobacteria) and fungi (Ascomycota and Basidiomycota), which include different genera namely Achromobacter, Arthrobacter, Aspergillus, Azospirillum, Azotobacter, Bacillus, Beijerinckia, Burkholderia, Enterobacter, Erwinia, Flavobacterium, Gluconoacetobacter, Haloarcula, Herbaspirillum, Methylobacterium, Paenibacillus, Pantoea, Penicillium, Piriformospora, Planomonospora, Pseudomonas, Rhizobium, Serratia and Streptomyces. These PGP microbes could be used as biofertilizers/bioinoculants at place of chemical fertilizers for sustainable agriculture. The aim of “Plant Microbiomes for Sustainable Agriculture” is to provide the current developments in the understanding of microbial diversity associated with plant systems in the form of rhizospheric, endophytic and epiphytic. The book is useful to scientist, research and students related to microbiology, biotechnology, agriculture, molecular biology, environmental biology and related subjects.
Plant Microevolution and Conservation in Human-Influenced Ecosystems
by David BriggsAs human activities are increasingly domesticating the Earth's ecosystems, new selection pressures are acting to produce winners and losers amongst our wildlife. With particular emphasis on plants, Briggs examines the implications of human influences on micro-evolutionary processes in different groups of organisms, including wild, weedy, invasive, feral, and endangered species. Using case studies from around the world, he argues that Darwinian evolution is ongoing. He considers how far it is possible to conserve endangered species and threatened ecosystems through management, and questions the extent to which damaged landscapes and their plant and animal communities can be precisely recreated or restored. Many of Darwin's ideas are highlighted, including his insights into natural selection, speciation, the vulnerability of rare organisms, the impact of invasive species, and the effects of climate change on organisms. An important text for students and researchers of evolution, conservation, climate change and sustainable use of resources.
Plant Micronutrients: Deficiency and Toxicity Management
by Khalid Rehman Hakeem Tariq AftabPlants require essential nutrients (macronutrients and micronutrients) for normal functioning. Sufficiency range is the levels of nutrients necessary to meet the plant’s needs for optimal growth. This range depends on individual plant species and the particular nutrient. Nutrient levels outside of a plant’s sufficiency range cause overall crop growth and health to decline, due either to deficiency or toxicity from over-accumulation. Apart from micronutrients (B, Cl, Mn, Fe, Zn, Cu and Mo), Aluminum (Al), cerium (Ce), cobalt (Co), iodine (I), lanthanum (La), sodium (Na), selenium (Se), silicon (Si), titanium (Ti), and vanadium (V) are emerging as novel biostimulants that may enhance crop productivity and nutritional quality. These beneficial elements are not "essential" but when supplied at low dosages, they augment plant growth, development, and yield by stimulating specific molecular, biochemical, and physiological pathways in responses to challenging environments.The book is the first reference volume that approaches plant micronutrient management with the latest biotechnological and omics tools. Expertly curated chapters highlight working solutions as well as open problems and future challenges in plant micronutrient deficiency or toxicity. We believe this book will introduce readers to state-of-the-art developments and research trends in this field.
Plant microRNAs: Shaping Development and Environmental Responses (Concepts and Strategies in Plant Sciences)
by Tamas Dalmay Célia Miguel Inês ChavesThis book summarizes the latest findings on the functions of microRNAs in the regulation of plant development and responses to the surrounding environment. MicroRNAs are an important class of molecules that can be found in diverse groups of organisms, including plants and animals, and the investigation of their roles is a highly dynamic and “hot” research topic. The respective chapters address four main aspects, namely: microRNA investigation and annotation, the regulatory roles of microRNAs in various developmental processes, in response to abiotic factors, and in the context of biotic stress response regulation. Systematically reviewing the most important findings in this field, the book offers an essential guide for undergraduate and graduate students, teachers, and plant science researchers. Due to the potential applications of microRNAs in crop breeding and plant protection, it also represents a valuable resource for scientists in academia and the private sector alike.
Plant MicroRNAs
by Pamela J. Green Blake C. MeyersMicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular BiologyTM series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike.
Plant MicroRNAs and Stress Response
by Deepu Pandita Anu PanditaMicroRNAs (miRNAs) are small (20–24 nt), single stranded, regulatory RNA molecules or gene regulators of critical transcriptional or post-transcriptional gene regulation in plants in sequence-specific order that respond to numerous abiotic stresses and animals, non-coding, highly evolutionarily conserved and widely distributed throughout the plant kingdom. MiRNAs are master regulators of plant growth and development, development attenuation under various environmental stresses by stress-responsive miRNAs and plant stress responses and tolerance. Drought, salinity, heat, cold, UV radiation, heavy metal, pathogens, pests and other microbial infections affect survival, growth, development, quality, yield, and production of plants. Stress induced miRNAs down regulate their target miRNAs. This down regulation leads to the accumulation and function of positive regulators, highlighting their roles in stress responses and tolerance. Plant miRNA mediated modifications include overexpression or repression of stress-responsive miRNAs and/or their target complementary or partially complementary gene products, miRNA-resistant target genes, target-mimics and artificial miRNAs. Thus, miRNAs may serve as "genomic gold mines", novel, potent and potential targets in plant genetic manipulations and miRNA-based biotechnology will aid plant improvement and crop-plant tolerance to different environmental stresses. This book reviews our recent understanding of plant microRNAs, biogenesis and functions, computational tools and bioinformatics, regulation of plant growth and development, expression studies, and the role of plant miRNAs in various biotic and abiotic stress-response regulation in plants.
Plant Microtechniques and Protocols
by Edward Chee Tak Yeung Claudio Stasolla Michael John Sumner Bing Quan HuangA proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one's own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e. g. , Jensen (1964); O'Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e. g. , archeology, wood science, etc. ]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.