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Plant Stress Tolerance: Molecular Mechanisms and Breeding Strategies, Volume 2

by Jen-Tsung Chen

Plant Stress Tolerance: Molecular Mechanisms and Breeding Strategies, Volume Two explores methods of precise management of biotic stressors including pests and pathogens. This is based on advanced molecular technologies including mutagenesis, genetic engineering, genome-wide association study, marker-assisted selection, genomic selection, molecular marker-based platforms, functional genomics, multiple omics tools, high-throughput technologies, computational biology, epigenetic manipulation, and clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing.This book proposes strategies involving immunity-boosting through releasing genetic resources from naturally resistant plants, regulating defense systems by phytohormones, promoting disease tolerance by biostimulants, and nanotechnology such as nanocarriers for managing biotic stressors. These positive approaches help to advance and accelerate breeding programs for disease-tolerant crops against various pests and pathogens.Plant Stress Tolerance: Molecular Mechanisms and Breeding Strategies, Volume Two is an ideal reference for the research fields of plant pathology, plant disease management, plant physiology, plant functional genomics, multiple omics, systems biology, and crop breeding. The book inspires ideas from the reader regarding future research on disease-resilient crops to face the challenge of global climate change and the increasing human population.

Plant Stress Tolerance

by Kareem A. Mosa Ahmed Ismail Mohamed Helmy

Providing a comprehensive overview of cutting-edge research on Omics applications in plant sciences field,"Plant Stress Tolerance" focuses on different approaches towards plant stress tolerance including both biotic stresses and abiotic stresses. This book outlines the challenges facing this area of research, with solid, up-to-date information for graduate students, academic scientists and researchers on using the recent advances of Omics technologies on plant stresses.

Plant Stress Tolerance

by Ramanjulu Sunkar

A number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical. In Plant Stress Tolerance: Methods and Protocols, expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results. Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Plant Stress Tolerance: Methods and Protocols provides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.

Plant Stress Tolerance: Methods and Protocols (Methods in Molecular Biology #2832)

by Ramanjulu Sunkar

This fully updated new edition explores new techniques for studying plant stress. This includes novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase–seq for identifying nucleosome occupancy, alternative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted metabolomics, ROS and NO measurements, priming-related protocols, growth-promoting bacteria isolation and functional characterization, as well as isolating mutants for stress-regulated genes using CRISPR technology. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Stress Tolerance: Methods and Protocols, Third Edition provides a wide range of protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.

Plant Stress Tolerance

by Ramanjulu Sunkar

A number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical. In Plant Stress Tolerance: Methods and Protocols, expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results. Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Plant Stress Tolerance: Methods and Protocols provides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.

Plant Structural Biology: Hormonal Regulations

by Jan Hejátko Toshio Hakoshima

After decades of dominance of genetics and genomics, the importance of structural biology is growing exponentially in the field of plant biology. The main objectives of this new book series is to “demystify” structural biology for plant researchers and to provide important insights into the basic molecular mechanisms underlying plant development through the diverse approaches utilized by structural biologists. The book series starts with a theme dedicated to hormonal signaling that has benefited from the application of structural biology. “Plant Structural Biology: Hormonal Regulations” provides up-to-date knowledge of the structural aspects of hormonal signal recognition, signal transduction, hormonal control of downstream regulatory pathways and hormonal crosstalk. The most distinctive features of this book as well as future titles is/will be to provide overview of cutting-edge research in the field of plant structural biology, and to serve as a compendium of various approaches that could be applied to problems being solved in modern plant biology. Last but not least, we hope this book will facilitate and broaden the community of (not only) plant scientists who are interested in structural biology approaches and tools. For these reasons, the style of this series is concise and general, in order to avoiding unnecessary details. Explanatory boxes describing the basics of specific approaches (e.g. X-ray crystallography, NMR, SAXS, molecular dynamics simulations, etc.) are included.

Plant Structure: A Colour Guide (Manson Ser.)

by Bryan G. Bowes James D. Mauseth

This book is a fundamental guide to understanding plant structure offering plant scientists, plant biologists and horticulturalists in practice, academic life and in training. It includes a combination of concise scientific text and superb color photographs and drawings, focusing on structure at anatomical, histological and fine structure levels.

Plant Synthetic Biology: Methods and Protocols (Methods in Molecular Biology #2379)

by Matias D. Zurbriggen

This volume provides methods on different aspects and applications on plants, algae, photosynthetic bacteria, synthetic construct design, and multiplex cloning. Chapters cover multiple aspects of synthetic metabolic, photosynthetic systems, metabolic and signaling pathways, advanced engineering of metabolic networks, isolation of organelles and co-culture of microorganisms, and methods for the on command manipulation of the relative stability of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Synthetic Biology: Methods and Protocols aims to be a useful resource for both researchers starting to explore novel experimental avenues as well as for experts willing to expand their portfolio of tools and strategies.

Plant Synthetic Promoters

by Reinhard Hehl

This book assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of cis-regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented. The experimental verification of the proposed expression profile conferred by the cis-regulatory elements requires the assembly of synthetic promoters. Several chapters are dedicated to the assembly of synthetic promoters, also including specific software tools to facilitate promoter design. Transient and transgenic reporter gene technology is a prominent approach to test the spatial and temporal expression driven by synthetic promoters, and several chapters address this approach. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice to ensure successful results in the lab. Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.

Plant Systematics

by Arun K. Pandey Shruti Kasana

This book is designed to introduce the fundamentals of systematics in a simple, concise and balanced manner. The book aims to equip the students with the basics of plant taxonomy and at the same time also update them with the most recent advances in the field of plant systematics. The book has been organized into 21 chapters that introduce and explain different concepts in a stimulating manner. The text is supplemented with relevant illustrations and photographs. Relevant literature has been added to provide a better picture of the most recent updates in the field of plant systematics. Note: T&F does not sell or distribute the Hardback in India, Pakistan, Nepal, Bhutan, Bangladesh and Sri Lanka.

Plant Systematics

by Michael G. Simpson

Plant Systematics has made a substantial contribution to plant systematics courses at the upper-undergraduate and first year graduate level. This second edition continues to provide the basis for teaching an introduction to the morphology, evolution, and classification of land plants. A foundation of the approach, methods, research goals, evidence, and terminology of plant systematics are presented along with the most recent knowledge of evolutionary relationships of plants and practical information vital to the field. In this second edition, the author includes greatly expanded treatments of families of lycophytes, ferns, gymnosperms, and flowering plants (all with full-color plates), a new chapter on species concepts and the role of systematics in conservation biology, and a new appendix summarizing basic statistical and morphometric techniques used in plant systematics studies. An explanation of maximum likelihood and Bayesian inference algorithms is included in methods of phylogenetic inference, and chapters on morphology and plant nomenclature have been augmented with new material.

Plant Systematics: An Integrated Approach, Fourth Edition

by Gurcharan Singh

This fourth edition of Plant Systematics is completely revised and updated. It incorporates the updated International Code of Nomenclature for Algae, Fungi and Plants (Shenzhen Code, 2018), the new version of PhyloCode (Beta version of Phylocode 5, 2014), APweb version 14 (September, 2018), revised Angiosperm Phylogeny Group classification (APG IV, 2016), new Pteridophyte Phylogeny Group Classification (PPG I, 2016), besides the updates since the publication of third edition. <P><P>The book is a blend of classical fundamental aspects and recent developments, especially in the field of molecular systematics, cladistics and computer identification. Special attention has been given to information on botanical nomenclature, identification, molecular systematics and phylogeny of angiosperms. The complicated concepts of phylogeny, taxometrics and cladistics have been explained with a view to providing a comparison between these diverse but interactive fields of study. An attempt has been made to build upon a common example when exploring different methods, especially in procedures of identification, taxometrics and cladistics. The major systems of classification are evaluated critically. Discussion on major families of Pteridophytes, Gymnosperms and Angiosperms, especially those of major phylogenetic interest, form a major portion of this edition. The ebook includes nearly 500 color photographs set out in 36 pages covering plants from different parts of the world. In addition, 305 black & white illustrations have been included to provide a better understanding of the plants covered in the book.

Plant Systems Biology

by Dmitry A. Belostotsky

As a fairly new and expansive area of study, plant systems biology has been approached by scientists from fields as varied as plant physiology and astrophysics, creating a wide variety of techniques and methods to further this vital research. In Plant Systems Biology, expert investigators provide cutting-edge chapters dealing with diverse subjects such as systems biology of plant gene expression to analysis of networks, pathways, specific statistical issues and novel computational tools, imaging-based tools as well as chemical genetic, metabolomic and integrative methods that cannot be easily pigeonholed. As a volume in the highly successful Methods in Molecular BiologyTM series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and comprehensive, Plant Systems Biology is an ideal guide for all researchers who wish to contribute to the evolution of the vibrant field of plant biology.

Plant Systems Biology: Methods and Protocols (Methods in Molecular Biology #2395)

by Mikaël Lucas

This second edition volume expands on the previous edition with an update on the latest techniques used to study plant systems biology on three specific scales: the molecular level, the tissue level, and the whole plant. Chapters cover topics such as gene regulatory network inference and dynamic modeling using ordinary differential equations or Boolean formalisms; protocols for at-will induction of plant aerial or root organs, or quantification of tissue mechanical properties; mathematical modeling of plant tissue using SimuPlant or VirtualLeaf; and simulating crop root systems using OpenSimRoot or R-SWMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, step-by-step, readily reproducible laboratory protocols or software tutorials, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Plants Systems Biology: Methods and Protocols, Second Edition is a valuable resource for plant biologists looking for different approaches to finding solutions to their questions and generating new ideas, as well as for students who desire to discover the field of plant systems biology.

Plant Taxonomy: The Systematic Evaluation of Comparative Data

by Tod F. Stuessy

The field of plant taxonomy has transformed rapidly over the past fifteen years, especially with regard to improvements in cladistic analysis and the use of new molecular data. The second edition of this popular resource reflects these far-reaching and dramatic developments with more than 3,000 new references and many new figures. Synthesizing current research and trends, Plant Taxonomy now provides the most up-to-date overview in relation to monographic, biodiversity, and evolutionary studies, and continues to be an essential resource for students and scholars.This text is divided into two parts: Part 1 explains the principles of taxonomy, including the importance of systematics, characters, concepts of categories, and different approaches to biological classification. Part 2 outlines the different types of data used in plant taxonomic studies with suggestions on their efficacy and modes of presentation and evaluation. This section also lists the equipment and financial resources required for gathering each type of data. References throughout the book illuminate the historical development of taxonomic terminology and philosophy while citations offer further study. Plant Taxonomy is also a personal story of what it means to be a practicing taxonomist and to view these activities within a meaningful conceptual framework. Tod F. Stuessy recalls the progression of his own work and shares his belief that the most creative taxonomy is done by those who have a strong conceptual grasp of their own research.

Plant Techniques: Theory and Practice

by S. Rama Rao S.M. Khasim K. Thammasiri M. Rahamtulla

This book deals with the basic concepts of Plant Science including botanical micro technique and microtomy, staining techniques, molecular techniques, plant tissue culture, electron microscopy, and cryopreservation and germplasm storage.It is the outcome of several decades of research and teaching in plant biology to undergraduate and postgraduate students of Plant Science, Horticulture, Microbiology, and Biotechnology.Print edition not for sale in Bangladesh, Bhutan, India, Nepal, Pakistan, and Sri Lanka.

Plant Theory

by Jeffrey Nealon

In our age of ecological disaster, this book joins the growing philosophical literature on vegetable life to ask how our present debates about biopower and animal studies change if we take plants as a linchpin for thinking about biopolitics. Logically enough, the book uses animal studies as a way into the subject, but it does so in unexpected ways. Upending critical approaches of biopolitical regimes, it argues that it is plants rather than animals that are the forgotten and abjected forms of life under humanist biopower. Indeed, biopolitical theory has consistently sidestepped the issue of vegetable life, and more recently, has been outright hostile to it. Provocatively, Jeffrey T. Nealon wonders whether animal studies, which has taken the "inventor" of biopower himself to task for speciesism, has not misread Foucault, thereby managing to extend humanist biopower rather than to curb its reach. Nealon is interested in how and why this is the case. Plant Theory turns to several other thinkers of the high theory generation in an effort to imagine new futures for the ongoing biopolitical debate.

Plant-Thinking: A Philosophy of Vegetal Life

by Michael Marder

The margins of philosophy are populated by non-human, non-animal living beings, including plants. While contemporary philosophers tend to refrain from raising ontological and ethical concerns with vegetal life, Michael Marder puts this life at the forefront of the current deconstruction of metaphysics. He identifies the existential features of plant behavior and the vegetal heritage of human thought so as to affirm the potential of vegetation to resist the logic of totalization and to exceed the narrow confines of instrumentality. Reconstructing the life of plants "after metaphysics," Marder focuses on their unique temporality, freedom, and material knowledge or wisdom. In his formulation, "plant-thinking" is the non-cognitive, non-ideational, and non-imagistic mode of thinking proper to plants, as much as the process of bringing human thought itself back to its roots and rendering it plantlike.

Plant Tissue Culture: Propagation, Conservation and Crop Improvement

by Mohammad Anis Naseem Ahmad

This book presents basic concepts, methodologies and applications of biotechnology for the conservation and propagation of aromatic, medicinal and other economic plants. It caters to the needs and challenges of researchers in plant biology, biotechnology, the medical sciences, pharmaceutical biotechnology and pharmacology areas by providing an accessible and cost-effective practical approach to micro-propagation and conservation strategies for plant species. It also includes illustrations describing a complete documentation of the results and research into particular plant species conducted by the authors over the past 5 years. Plant Biotechnology has been a subject of academic interest for a considerable time. In recent years, it has also become a useful tool in agriculture and medicine, as well as a popular area of biological research. Current economic growth is globally projected in a highly positive manner, but the challenges many countries face with regard to food, feed, malnutrition, infectious diseases, the newly identified life-style diseases, and energy shortages, all of which are worsened by an ever-deteriorating environment, continue to pull the growth digits back. The common thread that connects all of the above challenges is biotechnology, which could provide many answers. Molecular biology and biotechnology have now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenics now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. The book introduces several new and exciting areas to researchers who are unfamiliar with plant biotechnology and also serves as a review of ongoing research and future directions for scholars. The book highlights numerous methods for in vitro propagation and utilization of techniques in raising transgenics to help readers reproduce the experiments discussed.

Plant Tissue Culture: An Introductory Text

by Sant Saran Bhojwani Prem Kumar Dantu

Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.

Plant Tissue Culture

by Jayarama Reddy

This book is a comprehensive text on plant tissue culture, with its past, present, and future prospects and techniques discussed in detail. In the first three chapters, the history, terminologies, and applications are given in detail. The fourth chapter is dedicated to the instrumentation of plant tissue culture. The basic techniques used in PTC are described in the sixth chapter. The details of the constituents and types of different nutrient media are discussed in the eighth chapter. In chapter number 9, methods of haploid production have been described. Bioreactors are the instruments that are used for the large-scale production of plantlets and plant products. This book is useful for all the students, researchers, teachers, and industrialists interested in plant tissue culture. Print edition not for sale in india. This book is a comprehensive text on plant tissue culture, with its past, present, and future prospects and techniques discussed in detail.

Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition

by Robert N. Trigiano

Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.

Plant Tissue Culture, Development, and Biotechnology

by Robert N. Trigiano Dennis J. Gray

Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol

Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region

by Duong Tan Nhut Hoang Thanh Tung Edward Chee-Tak YEUNG

This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.

Plant Tolerance to Environmental Stress: Role of Phytoprotectants

by Mirza Hasanuzzaman Masayuki Fujita Hirosuke Oku M. Tofazzal Islam

Global climate change affects crop production through altered weather patterns and increased environmental stresses. Such stresses include soil salinity, drought, flooding, metal/metalloid toxicity, pollution, and extreme temperatures. The variability of these environmental conditions pared with the sessile lifestyle of plants contribute to high exposure to these stress factors. Increasing tolerance of crop plants to abiotic stresses is needed to fulfill increased food needs of the population. This book focuses on methods of improving plants tolerance to abiotic stresses. It provides information on how protective agents, including exogenous phytoprotectants, can mitigate abiotic stressors affecting plants. The application of various phytoprotectants has become one of the most effective approaches in enhancing the tolerance of plants to these stresses. Phytoprotectants are discussed in detail including information on osmoprotectants, antioxidants, phytohormones, nitric oxide, polyamines, amino acids, and nutrient elements of plants. Providing a valuable resource of information on phytoprotectants, this book is useful in diverse areas of life sciences including agronomy, plant physiology, cell biology, environmental sciences, and biotechnology.

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