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Ribbon of Darkness: Inferencing from the Shadowy Arts and Sciences
by Barbara Maria StaffordOver the course of her career, Barbara Stafford has established herself the preeminent scholar of the intersections of the arts and sciences, articulating new theories and methods for understanding the sublime, the mysterious, the inscrutable. Omnivorous in her research, she has published work that embraces neuroscience and philosophy, biology and culture, pinpointing connections among each discipline’s parallel concerns. Ribbon of Darkness is a monument to the scope of her work and the range of her intellect. At times associative, but always incisive, the essays in this new volume take on a distinctly contemporary purpose: to uncover the ethical force and moral aspects of overlapping scientific and creative inquiries. This shared territory, Stafford argues, offers important insights into—and clarifications of—current dilemmas about personhood, the supposedly menial nature of manual skill, the questionable borderlands of gene editing, the potentially refining value of dualism, and the limits of a materialist worldview. Stafford organizes these essays around three concepts that structure the book: inscrutability, ineffability, and intuitability. All three, she explains, allow us to examine how both the arts and the sciences imaginatively infer meaning from the “veiled behavior of matter,” bringing these historically divided subjects into a shared intellectual inquiry and imbuing them with an ethical urgency. A vanguard work at the intersection of the arts and sciences, this book will be sure to guide readers from either realm into unfamiliar yet undeniably fertile territory.
Ribonuclease P
by Fenyong Liu Sidney AltmanThis volume will discuss all important aspects of RNase P structure and function in eukaryotic, prokaryotic, and viral systems. It will cover a broad range of subjects in RNA research, including: structural analysis by biochemical or biophysical means; mRNA structure, function and biogenesis; ribosome structure and function; translational control; and RNA catalysis. RNA and RNase is an interdisciplinary topic that represents molecular, evolutionary and structural biology, biochemistry, biomedical sciences, chemistry, genetics, and virology as they relate to questions of the structure and function of RNA and of ribonucleoprotein assemblies.
Ribonucleases
by Allen W. NicholsonRibonucleases are a ubiquitous and functionally diverse group of enzymes that have a common ability to cleave RNA. Either through scission of internal phosphodiesters, or removal of nucleotides from RNA 5' or 3' ends, ribonucleases perform essential roles in gene expression and regulation, genome replication and maintenance, host defense, stress response, and viral strategies of infection. Ribonucleases have also served as highly informative models to understand virtually every aspect of biomolecular structure and function. The fifteen chapters in this volume are written by recognized researchers in the field, and provide in-depth analyses of the major ribonuclease families. Particular focus is given to the relation of ribonuclease structure and mechanism to biological function, as well as ribonuclease dysfunction in certain disease states. Other topics include the evolutionary genetics and functional diversification of ribonucleases, engineered ribonucleases as anti-cancer agents, the mechanisms of action of artificial ribonucleases, and ribonucleases as models to understand protein folding and stability. This volume should serve as an essential reference for a broad range of researchers and educators with interests in RNA metabolism, enzymology, and gene regulation.
Riboregulator Design and Analysis (Methods in Molecular Biology #2518)
by James Chappell Melissa K. TakahashiThis collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) regulator systems, the book continues with sections on techniques to create switchable riboregulator systems known as riboswitches, technologies that leverage RNA-guided CRISPR-Cas systems to edit the epigenome, control gene expression, and create diagnostics, as well as computational and experimental techniques to investigate the sequence-structure-function relationship of RNA systems that can both advance fundamental understanding and rational design of riboregulators. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Riboregulator Design and Analysis is an ideal guide for scientists and engineers interested in the design and application of riboregulators and driving further innovation in the field.
Ribosome Display and Related Technologies
by Ronald H. Jackson Julie A. DouthwaiteDisplay technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less widespread. Since this is in part due to the complexity of the methods, Ribosome Display and Related Technologies: Methods and Protocols seeks to extend their use by collecting expert contributions describing these detailed protocols. The protocols described range from well-established methods that have been used for a decade to generate high affinity antibodies, which are already in the clinic, to methods that are in their early stages of application such as display of peptides incorporating non-canonical amino acids. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Invaluable and easy to use, Ribosome Display and Related Technologies: Methods and Protocols will be of great benefit to those with general molecular biology or protein engineering experience who wish to select peptides or proteins by display, those with phage display experience who would benefit from the application of ribosome display, as well as those with some ribosome display experience who would like to expand the range of applications to which they are applying the technology.
Ribosome-inactivating Proteins
by Fiorenzo Stirpe Douglas LappiThis important reference provides up-to-date information on all aspects of ribosome-inactivating proteins (RIPs). Including a list of all known RIPs, their distribution in nature, structure, genetics and chemical and immunological properties, this reference covers mechanisms of action, including the enzymatic activity on various polynucleotide substrates; the interaction with, and entry into cells; the toxicity to animals, including the pathology of poisoning; and the immunomodulatory and allergenic activity. The book further emphasizes the use of immunotoxins and other conjugates in clinical trials for the therapy of cancer and intractable pain.
Ribosome Profiling: Methods and Protocols (Methods in Molecular Biology #2252)
by Vyacheslav M. LabunskyyThis book provides a single-source reference on the current state of the ribosome profiling method by describing experimental protocols for the quantitative analysis of translation in a variety of model organisms. In addition, the volume presents a detailed overview of the existing software tools and includes detailed description of methods for statistical analysis, data processing, and visualization of ribosome profiling data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ribosome Profiling: Methods and Protocols aims to provide the type of standardized protocols that have previously been unavailable in an effort to bypass the major barriers to wide use of ribosome profiling-based approaches.
Riboswitches
by Alexander SerganovThe revolutionary discoveries of RNA interference and bacterial regulatory RNAs led to further breakthroughs such as the identification of riboswitches and related RNA sensors, mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physical or chemical cues. In Riboswitches: Methods and Protocols, expert researchers provide comprehensive and up-to-date coverage of various methods used to study riboswitches and other RNAs involved in gene expression control. Examining biochemical and modern biophysical techniques, the volume focuses on mRNAs responding to small organic molecules but expands the definition of a riboswitch to incorporate classes of RNA that undergo conformational transitions in response to other stimuli in order to control the expression of genes. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Riboswitches: Methods and Protocols promises to inspire both novices and experts working in a wide range of contemporary biological areas who wish to further develop their RNA methodology.
Ribozyme Protocols
by Philip C. TurnerA comprehensive collection of essential and easily reproducible techniques currently used by leading researchers to manipulate RNA molecules and to inactivate gene expression in vivo. The methods range from theoretical design to clinical application and include the synthesis and assay of ribozymes, as well as their use both in vitro and in vivo. Each method is presented by an expert with substantial hands-on experience and in far greater detail than is to be generally found in the research literature. The book will be indispensable not only to those workers new to the field, but also to experienced researchers who want to expand their range of expertise.
Ribozymes
by Jörg S. HartigOver the past decade, there have been a variety of fundamental insights into ribozymes which have changed the perspective on RNA catalysis. In, Ribozymes: Methods and Protocols, researchers in the field detail many of the protocols and techniques which are now commonly used to study individual ribozymes and the characterization of ribozymes. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ribozymes: Methods and Protocols seeks to aid scientists in further understanding the catalytically active RNAs .
Ribozymes: Principles, Methods, Applications
by Sabine MüllerRibozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.
Ribozymes and siRNA protocols
by Mouldy SioudIn this completely updated and expanded edition of a classic bench manual, hands-on experts take advantage of the latest advances in ribozyme, DNAzyme, and RNA interference technologies to describe in detail the exciting and successful methods now available for gene inactivation in vitro and in vivo. Their optimized techniques employ hairpain ribozymes, DNAzymes, hammerhead ribozymes and derivatives, group I intron ribozymes, Rnase P ribozymes, and siRNAs, as well as general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy. Also provided are novel methods for identifying accessible cellular mRNA sites; group I intron and RNAse P ribozymes protocols for effective design, selection, and therapeutic applications; and the latest RNAi methods for sequencing-specific gene silencing in a wide variety of organisms. Comprehensive and up-to-date, Ribozymes and siRNA Protocols synthesizes for experienced and novice investigators alike the exciting advances in understanding nucleic acid enzymes and demonstrates how they may be used to analyze gene function and target validation, and to productively develop new therapeutics for human diseases.
Rice: Methods and Protocols (Methods in Molecular Biology #2869)
by Taiji KawakatsuThis volume aims to be a comprehensive guide to the field of rice molecular biology, encompassing both time-honored techniques and state-of-the-art methodologies. Chapters detail sample preparation, temporal and spatial analysis of RNA, protein expression, transformation and precise genome editing, scalable transcriptome analysis, epigenome analysis, and gene regulatory network analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Rice: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Rice Biofortification: Lessons for Global Science and Development
by Sally BrooksBiofortification - the enrichment of staple food crops with essential micronutrients - has been heralded as a uniquely sustainable solution to the problem of micronutrient deficiency or 'hidden hunger'. Considerable attention and resources are being directed towards the biofortification of rice - the world's most important food crop. Through an in-depth analysis of international rice biofortification efforts across the US, Philippines and China, this book provides an important critique of such goal-oriented, top-down approaches. These approaches, the author argues, exemplify a model of global, 'public goods' science that is emerging within complex, international research networks. It provides vital lessons for those researching and making decisions about science and research policy, showing that if this model becomes entrenched, it is likely to channel resources towards the search for 'silver bullet' solutions at the expense of more incremental approaches that respond to locality, diversity and the complex and uncertain interactions between people and their environments. The author proposes a series of key changes to institutions and practices that might allow more context-responsive alternatives to emerge. These issues are particularly important now as increasing concerns over food security are leading donors and policy makers to commit to ambitious visions of 'impact at scale' - visions which may never become a reality and may preclude more effective pathways from being pursued. Published in association with the Economic and Social Research Council (ESRC)
Rice By-products: Phytochemicals and Food Products Application
by Bee Ling Tan Mohd Esa NorhaizanRice is a vitally important staple food for almost half of the world’s population. As the global population increases, the demands for rice are expected to remain high. Since the rice industry will remain sustainable for a long time, the production of rice by-products will remain high. Substantial evidence suggests that rice by-products such as rice husk, rice straw, broken rice, rice germ, rice bran, and brewers’ rice may possess beneficial effects against oxidative stress and metabolic disorders. These beneficial effects have been linked to the phytochemicals present in rice by-products such as vitamin E, dietary fiber, γ-oryzanol, γ-aminobutyric acid (GABA), and phytosterols. Despite this evidence, the literature pertaining to rice by-products and its derived components has not well been compiled. To this end, Rice By-products: Phytochemicals and Food Products Application provides full coverage of issues pertaining to rice by-products, namely rice demands and rice by-products production, phytonutrients and antioxidant properties of rice by-products, potential health benefits, application in food products, and future prospects. By summarizing all the information in a lucid and comprehensive manner, authors provide a cohesive representation of the literature on the molecular mechanisms involved in the pharmacological effects of the bioactive components that present in rice by-products, as well as plausible means for the prevention of metabolic disorders for readers and allied stakeholders.
Rice Genome Engineering and Gene Editing: Methods and Protocols (Methods in Molecular Biology #2238)
by Anindya Bandyopadhyay Roger ThilmonyThis detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Rice Genome Engineering and Gene Editing: Methods and Protocols serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties.
Rice Genomics, Genetics and Breeding
by Takuji Sasaki Motoyuki AshikariThis book presents the latest advances in rice genomics, genetics and breeding, with a special focus on their importance for rice biology and how they are breathing new life into traditional genetics. Rice is the main staple food for more than half of the world’s population. Accordingly, sustainable rice production is a crucial issue, particularly in Asia and Africa, where the population continues to grow at an alarming rate. The book’s respective chapters offer new and timely perspectives on the synergistic effects of genomics and genetics in novel rice breeding approaches, which can help address the urgent issue of providing enough food for a global population that is expected to reach 9 billion by 2050.
Rice Grain Quality: Methods and Protocols (Methods in Molecular Biology #1892)
by Nese Sreenivasulu<p>This volume explores the latest advances in high-throughput phenotyping to screen milling quality, cooking and nutritional quality in breeding material, and simplifying grain quality screening in germplasm. The chapters in this book cover topics such as seed development stages; panicle architectural traits to understand yield components; starch structure and protein properties; and genome editing technologies to reveal grain quality and nutritional traits. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. <p>Cutting-edge and thorough, Rice Grain Quality: Methods and Protocols is a valuable resource for all researchers interested in expanding their knowledge in this evolving field.</p>
Rice Husk Biomass: Processing, Properties and Applications (Sustainable Materials and Technology)
by Mohammad Jawaid Brajendra ParmarThis book covers the processing, properties, and application of rice husk, also widely known as rice hull, which is an available agricultural waste in numerous rice-producing countries. These by-products are generated in significant quantities and must be treated and disposed of properly. Rice husk is an abundantly available waste material in all rice-producing countries. Approximately 120 million tons of rice husk is available each year after it has been removed from the whole rice paddy and is composed of 15% carbon, 18% ash, and 67% volatile matter. This book covers in-depth about processing and properties of rice husk and different by-products such as silica, rice husk ash, and nano fillers. This book also deals with various applications of rice husk in adsorbent, construction and building materials, composites, and nanocomposites. The book is a comprehensive reference resource for graduate students, early career researchers, scientists as well technologists working in the field of forestry, natural resources, material, and polymer sciences from concepts, fabrication, properties, and applications.
Rice Improvement: Physiological, Molecular Breeding and Genetic Perspectives
by Shabir Hussain Wani Jauhar AliThis book is open access under a CC BY 4.0 license.By 2050, human population is expected to reach 9.7 billion. The demand for increased food production needs to be met from ever reducing resources of land, water and other environmental constraints. Rice remains the staple food source for a majority of the global populations, but especially in Asia where ninety percent of rice is grown and consumed. Climate change continues to impose abiotic and biotic stresses that curtail rice quality and yields. Researchers have been challenged to provide innovative solutions to maintain, or even increase, rice production. Amongst them, the ‘green super rice’ breeding strategy has been successful for leading the development and release of multiple abiotic and biotic stress tolerant rice varieties. Recent advances in plant molecular biology and biotechnologies have led to the identification of stress responsive genes and signaling pathways, which open up new paradigms to augment rice productivity. Accordingly, transcription factors, protein kinases and enzymes for generating protective metabolites and proteins all contribute to an intricate network of events that guard and maintain cellular integrity. In addition, various quantitative trait loci associated with elevated stress tolerance have been cloned, resulting in the detection of novel genes for biotic and abiotic stress resistance. Mechanistic understanding of the genetic basis of traits, such as N and P use, is allowing rice researchers to engineer nutrient-efficient rice varieties, which would result in higher yields with lower inputs. Likewise, the research in micronutrients biosynthesis opens doors to genetic engineering of metabolic pathways to enhance micronutrients production. With third generation sequencing techniques on the horizon, exciting progress can be expected to vastly improve molecular markers for gene-trait associations forecast with increasing accuracy. This book emphasizes on the areas of rice science that attempt to overcome the foremost limitations in rice production. Our intention is to highlight research advances in the fields of physiology, molecular breeding and genetics, with a special focus on increasing productivity, improving biotic and abiotic stress tolerance and nutritional quality of rice.
Rice In The Tropics: A Guide To Development Of National Programs
by Robert F Chandler JrPublished in 1979. This book the first IADS sponsored volume on a commodity written especially for authorities, nonagricultural as well as agricultural, in developing countries and for the assistance agencies which cooperate with them. There are scores of commodities and problem areas for which comprehensive presentations of available information
Rice Protocols
by Yinong YangWith the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice. To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics. These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of "omics" techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.
Rice Research for Quality Improvement: Volume 2: Nutrient Biofortification and Herbicide and Biotic Stress Resistance in Rice
by Aryadeep RoychoudhuryThis book focuses on the conventional breeding approach, and on the latest high-throughput genomics tools and genetic engineering / biotechnological interventions used to improve rice quality. It is the first book to exclusively focus on rice as a major food crop and the application of genomics and genetic engineering approaches to achieve enhanced rice quality in terms of tolerance to various abiotic stresses, resistance to biotic stresses, herbicide resistance, nutritional value, photosynthetic performance, nitrogen use efficiency, and grain yield. The range of topics is quite broad and exhaustive, making the book an essential reference guide for researchers and scientists around the globe who are working in the field of rice genomics and biotechnology. In addition, it provides a road map for rice quality improvement that plant breeders and agriculturists can actively consult to achieve better crop production.
Rice Research for Quality Improvement: Volume 1: Breeding Techniques and Abiotic Stress Tolerance
by Aryadeep RoychoudhuryThis book focuses on the conventional breeding approach, and on the latest high-throughput genomics tools and genetic engineering / biotechnological interventions used to improve rice quality. It is the first book to exclusively focus on rice as a major food crop and the application of genomics and genetic engineering approaches to achieve enhanced rice quality in terms of tolerance to various abiotic stresses, resistance to biotic stresses, herbicide resistance, nutritional value, photosynthetic performance, nitrogen use efficiency, and grain yield. The range of topics is quite broad and exhaustive, making the book an essential reference guide for researchers and scientists around the globe who are working in the field of rice genomics and biotechnology. In addition, it provides a road map for rice quality improvement that plant breeders and agriculturists can actively consult to achieve better crop production.