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Transgenic Insects: Techniques and Applications (CABI Biotechnology Series #3)
by Hassan M. Ahmed Omar S. Akbar Serap Aksoy Yehonatan Alcalay Luke Alphey Yael Arien Rotem Daniel Avraham Camilla Beech Nicholas J. Bongio Vanessa Bottino-Rojas L. Roman Carrasco Lucy Carter Jackson Champer Isabelle Coche Carolina Concha Rocco D’Amato Brinda Dass Jason A. Delborne Ellen M. Dotson Ravi V. Durvasula Matthew P. Edgington Claudia Emerson Guido Favia Adam P. Forshaw Alexander W.E. Franz Marisa L. Guido Monika Gulia-Nuss Daniella An Haber Andrew Hammond Alfred M. Handler Keith R. Hayes Ivy Hurwitz Anthony A. James Thomas P. Kelly Nicole Klein Ana Kormos Lee Benjamin Lamdan David J. Lampe Rosemary S. Lees Maria Vittoria Mancini Aditi Mankad John Mumford Tony Nolan Andrew Nuss David A. O’Brochta Riccardo Papa Philippos Aris Papathanos Michael Pham Ricardo Pereira Megan Quinlan Robyn Raban Jason L. Rasgon Aaron Roberts Yasha Rohwer Nathan Rose Marc F. Schetelig Kristof De Schutter Hideki Sezutsu Arvind Sharma Gregory S. Simmons Guy Smagghe Toshiki Tamura Delphine Thizy Lea Pare Toe Brian L. Weiss Ernst A. Wimmer Liu YangTechnology for modifying the genotypes and phenotypes of insects and other arthropods has steadily progressed with the development of more precise and powerful methods, most prominently transgenic modification. For many insect pests, there is now almost unlimited ability to modify phenotypes to benefit human health and agriculture. Precise DNA modifications and gene drive have the power to make wild-type populations less harmful in ways that could never have been performed with previous transgenic approaches. This transition from primarily laboratory science to greater application for field use has also necessitated greater development of modeling, ethical considerations and regulatory oversight. The 2nd Edition of Transgenic Insects contains chapters contributed by experts in the field that cover technologies and applications that are now possible. This edition includes increased attention to associated challenges of risk assessment, regulation, and public engagement. Featuring: Up-to-date analysis of molecular techniques, such as gene editing. Consideration of public attitudes and regulatory aspects associated with transgenic insects. Many examples of the wide range of applications of transgenic insects. This book will be very valuable to students and researchers in entomology, molecular biology, genetics, public health and agriculture, and will also appeal to practitioners who are implementing the technology, and to regulators, stakeholders and ethicists.
Transgenic Insects: Techniques and Applications (CABI Biotechnology Series #3)
by Mark Q. BenedictInsect transgenesis promises improvements in agriculture, pharmaceuticals and public health. Many important insects can now be routinely transformed with effectors that have useful applications. Agriculture presents the largest market for transgenic insects and has a foundational history of success with sterile insect technique for control of pests including Mediterranean fruit flies and screwworms. Biotechnology will contribute superior markers, suppressible sterility and sex-conversion. Public health is also seeing transgenic mosquitoes developed which suppress natural populations and are incapable of transmitting disease. Experts in the field will contribute their insights into the latest technology and its applications. Authors will also consider the larger risks, social and economic aspects of transgenic insects whose value must be proven in political, regulatory and public acceptance arenas.
Transgenic Insects
by Mark Quentin BenedictInsect transgenesis promises improvements in agriculture, pharmaceuticals and public health. Many important insects can now be routinely transformed with effectors that have useful applications. Agriculture presents the largest market for transgenic insects and has a foundational history of success with sterile insect technique for control of pests including Mediterranean fruit flies and screwworms. Biotechnology will contribute superior markers, suppressible sterility and sex-conversion. Public health is also seeing transgenic mosquitoes developed which suppress natural populations and are incapable of transmitting disease. Experts in the field will contribute their insights into the latest technology and its applications. Authors will also consider the larger risks, social and economic aspects of transgenic insects whose value must be proven in political, regulatory and public acceptance arenas.
Transgenic Maize
by M. Paul ScottAs increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.
Transgenic Mouse: Methods and Protocols (Methods in Molecular Biology #2066)
by Melissa A. LarsonThis volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field.
Transgenic Mouse Methods and Protocols
by Marten H. Hofker Jan Van DeursenMarten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.
Transgenic Mouse Methods and Protocols, 2nd Edition
by Jan Van Deursen Marten H. HofkerMarten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.<P><P> Fully updated from the first edition to offer cutting-edge methods for generation of transgenic mouse models
Transgenic Plant Research
by Alan R. LindseyThis text is split into four main sections: gene transfer techniques; transgenic approaches to gene isolation; manipulation of plant development, biochemistry and physiology; and predictability of transgene expression.
Transgenic Plants
by Leandro PeñaA collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative real-time PCR, RT-PCR, in situ hybridization, FISH, and TAIL-PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Transgenic Plants: Methods and Protocols (Methods in Molecular Biology #1864)
by Michelle Smith Pierluigi Barone Sandeep KumarThis book provides thorough coverage of transgenic plants with methods on plant transformation, biotechnological application of transgenic plants, and future developments. Chapters are grouped into sections focusing on transformation model and crop plants, genome engineering, and transgenic event characterization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Transgenic Plants: Methods and Protocols aims to broaden the utility for readers, provide additional references for further understanding, and present the technology’s potential for solving some of our most urgent global challenges in food security.
Transgenic Plants
by Andy C Wetten Jim M. DunwellIn 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edition expert researchers in the field provide key techniques to investigate production and analysis of transgenic plants. Focusing on selection and detection methods, transformation technology, gene targeting, silencing and directed mutation, metabolic engineering and pharming, the book encompasses protocols relating to major crops and model plants being used for genomic analysis. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Transgenic Plants: Methods and Protocols, Second Edition aids scientists in the continuous improvements being made for the production and analysis of transgenic plants.
Transgenic Wheat, Barley and Oats
by Huw D. Jones Peter R. ShewryThe understanding of the physical and genetic structure of plant genomes and the determination of phenotypes via interactions of coding and non-coding regions with the environment has quickly become the key to the future of plant breeding and agriculture. In Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, expert investigators contribute the latest protocols for the transformation, regeneration and selection of these three species, using both biolistics and Agrobacterium tumefaciens, in order to fill the need for techniques and resources in this vital area of research. Covering such topics as in vitro Agrobacterium co-cultivation, the manipulation of gene expression, and GM risk assessment, this versatile collection focuses on the study of temperate small grain cereals while many of its readily reproducible procedures could be easily adapted to accommodate other cereals and plants. The in-depth chapters adhere to the highly successful Methods in Molecular BiologyTM series format, featuring brief introductions to the subject, lists of the necessary materials and reagents, step-by-step laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Transgenic Wheat, Barley and Oats: Production and Characterization Protocols is an ideal guide for scientists pursuing this powerful, forward-thinking genetic strategy.
Transgovernance: Advancing Sustainability Governance
by Louis Meuleman'Transgovernance: Advancing Sustainability Governance' analyses the question what recent and ongoing changes in the relations between politics, science and media - together characterized as the emergence of a knowledge democracy - may imply for governance for sustainable development, on global and other levels of societal decision making, and the other way around: How can the discussion on sustainable development contribute to a knowledge democracy? How can concepts such as second modernity, reflexivity, configuration theory, (meta)governance theory and cultural theory contribute to a 'transgovernance' approach which goes beyond mainstream sustainability governance? This volume presents contributions from various angles: international relations, governance and metagovernance theory, (environmental) economics and innovation science. It offers challenging insights regarding institutions and transformation processes, and on the paradigms behind contemporary sustainability governance.This book gives the sustainability governance debate a new context. It transforms classical questions into new options for societal decision making and identifies starting points and strategies towards effective governance of transitions to sustainability.
Transhumanism: A Grimoire of Alchemical Agendas
by Joseph P. Farrell Scott D de HartThe ultimate question is no longer "who am I" or "why am I here." These questions were answered in the earliest civilizations by philosophers and priests. Today we live in an age of such rapid advances in technology and science that the ultimate question must be rephrased: what shall we be? This book investigates what may become of human civilization, who is setting the agenda for a trans-humanistic civilization, and why . The modern Victor Frankenstein holds a high political office, carries diplomatic immunity, and is most likely funded by the largest corporations worldwide. His method is ancient: alchemy. His fraternities are well known and their secrets are well kept, but his goal of times past and present is the same; he dares to become as god, genetically manipulating the seeds of the earth, the beasts on the fields, and to claim legal ownership over humanity by re-creating it in his own image. This is no fairy tale, science fiction, or conspiracy theory ... it simply is! Transhumanism, a Grimoire of Alchemical Agendas by Dr.'s. Joseph P. Farrell and Scott D. de Hart lifts the veil from the macabre transhumanistic monster being assembled and exposes the hidden history and agenda that has set humanity on a collision course for the Apocalypse. Joseph P. Farrell is the author of the best-selling Genes, Giants, Monsters, and Men: The Surviving Elites of the Cosmic War and Their Hidden Agenda.
Transhumanism: Evolutionary Futurism and the Human Technologies of Utopia
by Andrew PilschTranshumanism posits that humanity is on the verge of rapid evolutionary change as a result of emerging technologies and increased global consciousness. However, this insight is dismissed as a naive and controversial reframing of posthumanist thought, having also been vilified as “the most dangerous idea in the world” by Francis Fukuyama. In this book, Andrew Pilsch counters these critiques, arguing instead that transhumanism’s utopian rhetoric actively imagines radical new futures for the species and its habitat.Pilsch situates contemporary transhumanism within the longer history of a rhetorical mode he calls “evolutionary futurism” that unifies diverse texts, philosophies, and theories of science and technology that anticipate a radical explosion in humanity’s cognitive, physical, and cultural potentialities. By conceptualizing transhumanism as a rhetoric, as opposed to an obscure group of fringe figures, he explores the intersection of three major paradigms shaping contemporary Western intellectual life: cybernetics, evolutionary biology, and spiritualism. In analyzing this collision, his work traces the belief in a digital, evolutionary, and collective future through a broad range of texts written by theologians and mystics, biologists and computer scientists, political philosophers and economic thinkers, conceptual artists and Golden Age science fiction writers. Unearthing the long history of evolutionary futurism, Pilsch concludes, allows us to more clearly see the novel contributions that transhumanism offers for escaping our current geopolitical bind by inspiring radical utopian thought.
Transhumanism and Society
by Stephen LilleyThis book provides an introductory overview to the social debate over enhancement technologies with an overview of the transhumanists' call to bypass human nature and conservationists' argument in defense of it. The author present this controversy as it unfolds in the contest between transhumanists proponents and conservationists, who push back with an argument to conserve human nature and to ban enhancement technologies. This book provides an overview of the key contested points and present the debate in an orderly, constructive fashion. Readers are informed about the discussion over humanism, the tension between science and religion, and the interpretation of socio-technological revolutions; and are invited to make up their own mind about one of the most challenging topics concerning the social and ethical implications of technological advancements.
Transhumanism and the Image of God: Today's Technology and the Future of Christian Discipleship
by Jacob ShatzerWe're constantly invited to think about the future of technology as a progressive improvement of tools: our gadgets will continue to evolve, but we humans will stay basically the same. In the future, perhaps even alien species and intelligent robots will coexist alongside humans, who will grapple with challenges and emerge as the heroes. But the truth is that radical technological change has the power to radically shape humans as well. We must be well informed and thoughtful about the steps we're already taking toward a transhuman or even posthuman future. Can we find firm footing on a slippery slope? Biblical ethicist Jacob Shatzer guides us into careful consideration of the future of Christian discipleship in a disruptive technological environment. In Transhumanism and the Image of God, Shatzer explains the development and influence of the transhumanist movement, which promotes a "next stage" in human evolution. Exploring topics such as artificial intelligence, robotics, medical technology, and communications tools, he examines how everyday technological changes have already altered and continue to change the way we think, relate, and understand reality. By unpacking the doctrine of the incarnation and its implications for human identity, he helps us better understand the proper place of technology in the life of the disciple and avoid false promises of a posthumanist vision. We cannot think about technology use today without considering who we will become tomorrow.
Transhumanism - Engineering the Human Condition: History, Philosophy and Current Status (Springer Praxis Books)
by Roberto ManzoccoThis book is designed to offer a comprehensive high-level introduction to transhumanism, an international political and cultural movement that aims to produce a “paradigm shift” in our ethical and political understanding of human evolution. Transhumanist thinkers want the human species to take the course of evolution into its own hands, using advanced technologies currently under development – such as robotics, artificial intelligence, biotechnology, cognitive neurosciences, and nanotechnology – to overcome our present physical and mental limitations, improve our intelligence beyond the current maximum achievable level, acquire skills that are currently the preserve of other species, abolish involuntary aging and death, and ultimately achieve a post-human level of existence. The book covers transhumanism from a historical, philosophical, and scientific viewpoint, tracing its cultural roots, discussing the main philosophical, epistemological, and ethical issues, and reviewing the state of the art in scientific research on the topics of most interest to transhumanists. The writing style is clear and accessible for the general reader, but the book will also appeal to graduate and undergraduate students.
Transhumanist Dreams and Dystopian Nightmares: The Promise and Peril of Genetic Engineering (Bioethics)
by Maxwell J. MehlmanWhat will happen when technology allows us to direct our own evolution?Transhumanists advocate for the development and distribution of technologies that will enhance human intellectual, physical, and psychological capacities, even eliminate aging. What if the dystopian futures and transhumanist utopias found in the pages of science journals, Margaret Atwood novels, films like Gattaca, and television shows like Dark Angel are realized? What kind of world would humans have created? Maxwell J. Mehlman considers the promises and perils of using genetic engineering in an effort to direct the future course of human evolution. He addresses scientific and ethical issues without choosing sides in the dispute between transhumanists and their challengers. However, Transhumanist Dreams and Dystopian Nightmares reveals that radical forms of genetic engineering could become a reality much sooner than many people think, and that we need to encourage risk-management efforts. Whether scientists are dubious or optimistic about the prospects for directed evolution, they tend to agree on two things. First, however long it takes to perfect the necessary technology, it is inevitable that humans will attempt to control their evolutionary future, and second, in the process of learning how to direct evolution, we are bound to make mistakes. Our responsibility is to learn how to balance innovation with caution.
The Transhumanist Movement
by Francesco Paolo AdornoThe book is published as part of the "PRIN 2017 The Dark side of the Law".This volume analyzes the theoretical underpinnings of the academic transhumanism movement, beginning with the relationship between anthropology and technique. The author focuses on the question of immortality, which can be considered the core of transhumanism. The true depth of immortality will be discussed, through which and how many transformations could be produced in order to change our society, which is basically shaped by and for human mortal beings, in a society composed by immortal persons. Some writers have written about what a future populated with immortals might look like, which is far removed from both the bright future painted by transhumanists and from the disappearance of humanity feared by bioconservatives.
Transient and Strange: Notes on the Science of Life
by Nell GreenfieldboyceAn astonishing debut from the beloved NPR science correspondent: intimate essays about the intersection of science and everyday life. In her career as a science reporter, Nell Greenfieldboyce has reported from inside a space shuttle, the bottom of a coal mine, and the control room of a particle collider; she’s presented news on the color of dinosaur eggs, ice worms that live on mountaintop glaciers, and signs of life on Venus. In this, her debut book, she delivers a wholly original collection of powerful, emotionally raw, and unforgettable personal essays that probe the places where science touches our lives most intimately. Expertly weaving her own experiences of motherhood and marriage with an almost devotional attention to the natural world, Greenfieldboyce grapples with the weighty dualities of life: birth and death, constancy and impermanence, memory and doubt, love and aging. She looks for a connection to the universe by embarking on a search for the otherworldly glint of a micrometeorite in the dust, consults meteorologists and storm chasers on the eerie power of tornadoes to soothe her children’s anxieties, and processes her adolescent oblivion through the startling discovery of black holes. Inspired throughout by Walt Whitman’s invocation to the “transient and strange,” she remains attuned to the wildest workings of our world, reflecting on the incredible leap of the humble flea or the echoing truth of a fetal heartbeat. A beautiful blend of explanatory science, original reporting, and personal experience, Transient and Strange captures the ache of ordinary life, offering resonant insights into both the world around us and the worlds within us.
Transient Changes in Molecular Geometries and How to Model Them
by Asmus Ougaard DohnThis thesis examines various aspects of excess excitation energy dissipation via dynamic changes in molecular structure, vibrational modes and solvation. The computational work is carefully described and the results are compared to experimental data obtained using femtosecond spectroscopy and x-ray scattering. The level of agreement between theory and experiment is impressive and provides both a convincing validation of the method and significant new insights into the chemical dynamics and molecular determinants of the experimental data. Hence, the method presented in the thesis has the potential to become a very important contribution to the rapidly growing field of femtosecond x-ray science, a trend reflected in the several free-electron x-ray lasers (XFELs) currently being built around the world. Light-induced chemical processes are accompanied by molecular motion of electrons and nuclei on the femtosecond time scale. Uncovering these dynamics is central to our understanding of the chemical reaction on a fundamental level. Asmus O. Dohn has implemented a highly efficient QM/MM Direct Dynamics method for predicting the solvation dynamics of transition metal complexes in solution.
Transient Chaos
by Ying-Cheng Lai Tamás TélThe aim of this Book is to give an overview, based on the results of nearly three decades of intensive research, of transient chaos. One belief that motivates us to write this book is that, transient chaos may not have been appreciated even within the nonlinear-science community, let alone other scientific disciplines.
Transient Dynamics of Concentrated Particulate Suspensions Under Shear (Springer Theses)
by Endao HanThis thesis demonstrates the first use of high-speed ultrasound imaging to non-invasively probe how the interior of a dense suspension responds to impact. Suspensions of small solid particles in a simple liquid can generate a rich set of dynamic phenomena that are of fundamental scientific interest because they do not conform to the typical behavior expected of either solids or liquids. Most remarkable is the highly counter-intuitive ability of concentrated suspensions to strongly thicken and even solidify when sheared or impacted. The understanding of the mechanism driving this solidification is, however, still limited, especially for the important transient stage while the response develops as a function of time. In this thesis, high-speed ultrasound imaging is introduced to track, for the first time, the transition from the flowing to the solidified state and directly observe the shock-like shear fronts that accompany this transition. A model is developed that agrees quantitatively with the experimental measurements. The combination of imaging techniques, experimental design, and modeling in this thesis represents a major breakthrough for the understanding of the dynamic response of dense suspensions, with important implications for a wide range of applications ranging from the handling of slurries to additive manufacturing.
Transient Heat Transfer
by Donatello AnnaratoneThis book presents a new and direct computational method for transient heat transfer. The approach uses the well-known dimensionless Biot number and a second dimensionless number introduced by the author. The methodology allows for a transient heat transfer calculations without using finite difference programs. The book presents many examples and various tables demonstrating the potential of this new methodology. Many diagrams illustrate the physical phenomena.