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Yeast Protocols (Methods in Molecular Biology #2196)
by Wei XiaoThis fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community.
Yeast Strain Selection
by Chandra J. PanchalContributors from universities and food, pharmaceutical, and brewing companies detail the current state of yeast strain development and handling, highlighting advances in yeast selection for academic research, industry, and recombinant DNA technology. Featuring the use of Saccharomyces and other yea
Yeast Surface Display
by Bin LiuIn addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers.
Yeast Surface Display (Methods in Molecular Biology #2491)
by Michael W. TraxlmayrThis detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.
Yeast Systems Biology: Methods and Protocols (Methods in Molecular Biology #2049)
by Juan I. Castrillo Stephen G. OliverThis second edition volume expands on the previous edition with a look at the latest advances in techniques to study yeast and its core set of interactions, modules, architectures, and network dynamics that are common in all eukaryotes. The chapters in this book are organized into Four Parts: Part One provides readers with an update on the development of novel experimental and computational approaches to yeast systems biology; Part Two explores high-throughput methods used to study yeast epigenome, transcriptome, proteome, and metabolome; Part Three talks about computational systems biology, and focuses on data management, dynamic modeling, constraint-based models of metabolic networks, and multi-level ‘omics data; while Part Four looks at experimental platforms that utilize yeast to model systemic human diseases such as Alzheimer’s and Parkinson’s diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Yeast System Biology: Methods and Protocols, Second Edition is a valuable tool for graduate students, post-doctoral researchers, and experts who are interested in learning about the latest developments in the study of yeast.
Yeast Systems Biology
by Juan I. Castrillo Stephen G. OliverSystems Biology aims at deciphering the genotype-phenotype relationships at the levels of genes, transcripts (RNAs), peptides, proteins, metabolites, and environmental factors participating in complex cellular networks in order to reveal the mechanisms and principles governing the behavior of complex biological systems. Yeast Systems Biology: Methods and Protocols presents an up-to-date view of the optimal characteristics of the yeast Saccharomyces cerevisiae as a model eukaryote, perspective on the latest experimental and computational techniques for systems biology studies, most of which were first designed for and validated in yeast, and selected examples of yeast systems biology studies and their applications in biotechnology and medicine. These experiments under controlled conditions can uncover the complexity and interplay of biological networks with their dynamics, basic principles of internal organization, and balanced orchestrated functions between organelles in direct interaction with the environment as well as the characterization of short and long-term effects of perturbations and dysregulation of networks that may illuminate the origin of complex human diseases. Written for the highly successful Methods in Molecular BiologyTM series, this volume contains the kind of detailed description and implementation advice that is crucial for getting optimal results. Practical and cutting-edge, Yeast Systems Biology: Methods and Protocols serves researchers interested in comprehensive systems biology strategies in well-defined model systems with specific objectives as well as a better knowledge of the latest post-genomic strategies at all 'omic levels and computational approaches towards analysis, integration, and modeling of biological systems, from single-celled organisms to higher eukaryotes.
Yeasts in the Production of Wine
by Patrizia Romano Maurizio Ciani Graham H. FleetIt is well established that certain strains of yeasts are suitable for transforming grape sugars into alcohol, while other yeast strains are not suitable for grape fermentations. Recent progress has clearly demonstrated that the sensory profile of a wine is characteristic of each vine cultivated, and the quality and technological characteristics of the final product varies considerably due to the strains which have performed and/or dominated the fermentation process. Because of their technological properties, wine yeast strains differ significantly in their fermentation performance and in their contribution to the final bouquet and quality of wine, such as useful enzymatic activities and production of secondary compounds related both to wine organoleptic quality and human health. The wine industry is greatly interested in wine yeast strains with a range of specialized properties, but as the expression of these properties differs with the type and style of wine to be made, the actual trend is in the use of selected strains, which are more appropriate to optimize grape quality. Additionally, wine quality can be influenced by the potential growth and activity of undesirable yeast species, considered spoilage yeasts, which cause sluggish and stuck fermentation and detrimental taste and aroma in the wine.
Yellow Biotechnology I
by Andreas VilcinskasUtility of Insects for Studying Human Pathogens and Evaluating New Antimicrobial Agents, by Yan Wang, De-Dong Li, Yuan-Ying Jiang and Eleftherios Mylonakis. Galleria Mellonella as a Model Host to Study Gut Microbe Homeostasis and Brain Infection by the Human Pathogen Listeria Monocytogenes, by Krishnendu Mukherjee, Ramya Raju, Rainer Fischer and Andreas Vilcinskas. Drosophila as a Model to Study Metabolic Disorders, by Julia Hoffmann, Renja Romey, Christine Fink and Thomas Roeder. The Fruit Fly Drosophila melanogaster as a Model for Aging Research, by Annely Brandt and Andreas Vilcinskas. Drosophila and the Hallmarks of Cancer, by Theodoulakis Christofi and Yiorgos Apidianakis. The red flour beetle Tribolium castaneum as a model to monitor food safety and functionality, by Stefanie Grünwald, Iris V. Adam, Ana-Maria Gurmai, Ludmila Bauer, Michael Boll, and Uwe Wenzel. Identification and Bioanalysis of Natural Products from Insect Symbionts and Pathogens, by Alexander O. Brachmann and Helge B. Bode. Antiparasitic Peptides, by Jette Pretzel, Franziska Mohring, Stefan Rahlfs and Katja Becker.
Yellow Perch, Walleye, and Sauger: Aspects of Ecology, Management, and Culture (Fish & Fisheries Series #41)
by John Clay Bruner Robin L. DeBruyneWalleye, one of the most sought-after species of freshwater sport fishes in North America, has demonstrated appreciable declines in their numbers from their original populations since the beginning of the 20th century. Similarly, Yellow Perch, once the most commonly caught sport fish and an important commercial species in North America, have also shown declines. Compiling up-to-date information on the biology and management of Walleye, Sauger, and Yellow Perch, including research on systematics, genetics, physiology, ecology, movement, population dynamics, culture, recent case histories, and management practices, will be of interest to managers, researchers, and students who deal with these important species, particularly in light of habitat alterations, population shifts, and other biotic and abiotic factors related to a changing climate.
Yellowstone Wolves: Science and Discovery in the World's First National Park (America's Animal Comebacks Ser.)
by Douglas W. Smith Daniel R. MacNulty Daniel R. StahlerIn 2020, it will have been twenty-five years since one of the greatest wildlife conservation and restoration achievements of the twentieth century took place: the reintroduction of wolves to the world’s first national park, Yellowstone. Eradicated after the park was established, then absent for seventy years, these iconic carnivores returned to Yellowstone in 1995 when the US government reversed its century-old policy of extermination and—despite some political and cultural opposition—began the reintroduction of forty-one wild wolves from Canada and northwest Montana. In the intervening decades, scientists have studied their myriad behaviors, from predation to mating to wolf pup play, building a one-of-a-kind field study that has both allowed us to witness how the arrival of top predators can change an entire ecosystem and provided a critical window into impacts on prey, pack composition, and much else. Here, for the first time in a single book, is the incredible story of the wolves’ return to Yellowstone National Park as told by the very people responsible for their reintroduction, study, and management. Anchored in what we have learned from Yellowstone, highlighting the unique blend of research techniques that have given us this knowledge, and addressing the major issues that wolves still face today, this book is as wide-ranging and awe-inspiring as the Yellowstone restoration effort itself. We learn about individual wolves, population dynamics, wolf-prey relationships, genetics, disease, management and policy, newly studied behaviors and interactions with other species, and the rippling ecosystem effects wolves have had on Yellowstone’s wild and rare landscape. Perhaps most importantly of all, the book also offers solutions to ongoing controversies and debates. Featuring a foreword by Jane Goodall, beautiful images, a companion online documentary by celebrated filmmaker Bob Landis, and contributions from more than seventy wolf and wildlife conservation luminaries from Yellowstone and around the world, Yellowstone Wolves is a gripping, accessible celebration of the extraordinary Yellowstone Wolf Project—and of the park through which these majestic and important creatures once again roam.
Yellowstone's Wildlife in Transition
by P. J. White Robert A. Garrott Glenn E. PlumbThe world's first national park is constantly changing. How we understand and respond to recent events putting species under stress will determine the future of ecosystems millions of years in the making. Marshaling expertise from over 30 contributors, Yellowstone's Wildlife in Transition examines three primary challenges to the park's ecology.
Yersinia Enterocolitica
by Edward J. BottoneFirst published in 1981. Comprehensive overview of existing knowledge of Yersina enterocolitica, including laboratory models, clinical observations, and associated dieases.
Yersinia Pestis Protocols (Springer Series in Translational Stroke Research)
by Ruifu YangThis protocol book includes common and advanced research protocols for Yersinia pestis research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, gene deletion. It will be helpful for graduate students, clinicians, and researchers in infectious diseases, systems biology, and evolution and numerous other relevant fields.
Yet Another Introduction to Dark Matter: The Particle Physics Approach (Lecture Notes in Physics #959)
by Martin Bauer Tilman PlehnDark matter is a frequently discussed topic in contemporary particle physics. Written strictly in the language of particle physics and quantum field theory, these course-based lecture notes focus on a set of standard calculations that students need in order to understand weakly interacting dark matter candidates. After introducing some general features of these dark matter agents and their main competitors, the Higgs portal scalar and supersymmetric neutralinos are introduced as our default models. In turn, this serves as a basis for exploring four experimental aspects: the dark matter relic density extracted from the cosmic microwave background; indirect detection including the Fermi galactic center excess; direct detection; and collider searches. Alternative approaches, like an effective theory of dark matter and simplified models, naturally follow from the discussions of these four experimental directions.
Yąnomamö (Sixth Edition: The Legacy Edition) (Case Studies in Cultural Anthropology Series)
by Napoleon A. ChagnonThe Yanomamo of Venezuela and Brazil are a truly remarkable people, and one of the few sovereign tribal societies left on earth. This classic ethnography, based on the authors extensive fieldwork, includes a brief discussion of events and changes that have occurred since 1996. The Legacy 6th Edition of The Yanomamo also includes a Q&A interview with the author, which reveals his own perspective on his life's work, reflects changes within the field of anthropology itself, and presents the authors views on the recent decade of controversies that his work has inspired among critics (including some anthropologists).
Yo contengo multitudes: Los microbios que nos habitan y una mayor visión de la vida
by Ed YongYo contengo multitudes ya es considerado una obra fundamental de ciencia divulgativa -como lo fue en su momento El gen egoísta-. Un libro fascinante y rompedor que cambiará de mil millones de micromaneras nuestra percepción del mundo natural y el espacio que en él ocupamos. El cuerpo humano alberga billones de microbios que conforman todo un mundo en simbiosis con su entorno. Estos microscópicos y multitudinarios compañeros vitales no solo moldean nuestros órganos, nos protegen de enfermedades, e influyen en nuestro comportamiento, sino que resultan clave a la hora de entender el funcionamiento de la vida. En Yo contengo multitudes Ed Yong nos abre los ojos y nos invita con su erudición y sentido del humor a mirarnos como algo más que individuos: como receptáculos interdependientes de los microbiomas que conforman todos los seres vivos. Así, descubriremos la asombrosa e invisible ciencia detrás de los gigantescos arrecifes que construyen los corales, aprenderemos cómo ciertos calamares crean juegos de luces, y veremos el modo en que las bacterias pueden alterar nuestra respuesta en la lucha contra el cáncer, manipular nuestro sistema inmunológico, influir en nuestra evolución e incluso modificar nuestro genoma. Bill Gates opina sobre este libro...«Después de leer el interesantísimo libro Yo contengo multitudes del periodista británico Ed Yong, veo los microbios con una mirada diferente y hablo de ellos con nuevos términos. [...] Yong sintetiza literalmente cientos y cientos de páginas, sin abrumarte nunca con la ciencia. Tan solo imparte una visión fascinante y sorprendente detrás de otra. Yo contengo multitudes es el mejor periodismo científico.»The Blog of Bill Gates
Yo Wants to Know: All About Dinosaurs!
by Lea DanielYo has many questions about the dinosaurs at the museum. Good thing Grandpa is there to answer them!
Yo Wants to Know: How Old Is a Tree?
by Lea DanielGrandpa surprises Yo with a tree to plant for his birthday, so the two can grow tall together.
Yo Wants to Know: How Do Sound Waves Move?
by Lea DanielGrandma explains how sound waves help Yo the Magician make rice jump and dance!
Yo Wants to Know: Where Do Stars Go During the Day?
by Lea DanielDo you ever wonder where the stars go during the day? Yo is ready to fly into the sky in a rocket ship when he learns something from his Dad. Yo learns that even though he can’t see the stars during the day, they are always there.
Yo Wants to Know: What Happened to the Lights?
by Lea DanielHave you ever had a power outage in your house? The electricity in Yo’s neighborhood goes out, but his mom is prepared with emergency supplies. She makes a special treat and Yo’s dad tells a story to make the night less scary.
Yo Wants to Know: Why Are My Pupils So Big?
by Lea DanielHave you ever noticed that your eyes change when you’re in the dark? Yo pretends he is in a deep, dark cave and shows Jennifer what happens to his eyes in the light. Will Lips the dog’s eyes react the same way?
Yo Wants to Know: Why Do Possums Play Dead?
by Lea and Alan DanielWhat do you do when something scares you? When possums get scared and think a predator is threatening them, they play dead! Yo learns about possums and their survival instincts when he and his dad take their dog for a walk.
Yo Wants to Know: What Do You Smell?
by Lea and Alan DanielDid you know your nose can tell you if someone is baking some delicious cookies, or if milk has gone bad? Smell is one of our most important senses. It gives you clues to help you find food when you’re hungry and avoid food that will make you sick!