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Yearbook on Space Policy 2017: Security in Outer Space: Rising Stakes for Civilian Space Programmes (Yearbook on Space Policy)
by Giulia Bordacchini Edward BurgerThe book describes the recent trends in space policy and the space sector overall. While maintaining a global scope with a European perspective, it links space policy with other policy areas, highlights major events, and provides insights on the latest data. The Yearbook includes the proceedings of ESPI's 12th Autumn Conference, which discussed the growing importance of Security in Outer Space and the stakes for civilian space programmes in the public and private sectors. Bringing together satellite operators, SMEs, European and American institutions, and think tanks, the Autumn Conference served as platform for fresh insights on security in outer space and the potential of transatlantic relations to address its challenges. The Yearbook also includes executive summaries of ESPI's work in 2017 as well as ESPI's 2017 Executive Briefs, covering topics such as suborbital spaceflight, super heavy lift launch vehicles, collaboration with China, and the delimitation of outer space. All in all, the book gives a detailed review of space policy developments worldwide, contextualised with information about national-level space industries and activity and broader political and economic conditions. The readership is expected to include the staff of space agencies, the space industry, and the space law and policy research community.
Yeast: Molecular and Cell Biology
by Horst FeldmannFinally, a stand-alone, all-inclusive textbook on yeast biology. Based on the feedback resulting from his highly successful monograph, Horst Feldmann has totally rewritten he contents to produce a comprehensive, student-friendly textbook on the topic. The scope has been widened, with almost double the content so as to include all aspects of yeast biology, from genetics via cell biology right up to biotechnology applications. The cell and molecular biology sections have been vastly expanded, while information on other yeast species has been added, with contributions from additional authors. Naturally, the illustrations are in full color throughout, and the book is backed by a complimentary website. The resulting textbook caters to the needs of an increasing number of students in biomedical research, cell and molecular biology, microbiology and biotechnology who end up using yeast as an important tool or model organism.
Yeast: Molecular and Cell Biology
by Horst FeldmannYeast is one of the oldest domesticated organisms and has both industrial and domestic applications. In addition, it is very widely used as a eukaryotic model organism in biological research and has offered valuable knowledge of genetics and basic cellular processes. In fact, studies in yeast have offered insight in mechanisms underlying ageing and diseases such as Alzheimers, Parkinsons and cancer. Yeast is also widely used in the lab as a tool for many technologies such as two-hybrid analysis, high throughput protein purification and localization and gene expression profiling. The broad range of uses and applications of this organism undoubtedly shows that it is invalubale in research, technology and industry. Written by one of the world's experts in yeast, this book offers insight in yeast biology and its use in studying cellular mechanisms.
Yeast Cell Envelopes Biochemistry Biophysics and Ultrastructure: Volume II
by Leo H ArnoldA comprehensive review of the yeast cell envelope has not appeared previously and therefore this book is timely. The title of this volume was chosen to reflect the three major areas of contribution to our current understanding of the cell envelope, but we have not attempted to group chapters into subdivisions. The approach was to describe phenomena, to review the literature and to illuminate outstanding problems. It was also attempted to generate working hypotheses which may stimulate further studies. The some of these ideas be of germinal value is of more concern to us than that all of the hypotheses should stand the test of further experimentation.
Yeast Cell Envelopes Biochemistry Biophysics and Ultrastructure: Volume I
by Leo H ArnoldA comprehensive review of the yeast cell envelope has not appeared previously and therefore this book is timely. The title of this volume was chosen to reflect the three major areas of contribution to our current understanding of the cell envelope, but we have not attempted to group chapters into subdivisions. The approach was to describe phenomena, to review the literature and to illuminate outstanding problems. It was also attempted to generate working hypotheses which may stimulate further studies. The some of these ideas be of germinal value is of more concern to us than that all of the hypotheses should stand the test of further experimentation.
Yeast Cytokinesis
by Alberto Sanchez-Diaz Pilar PerezThis volume presents protocols on yeast cytokinesis that will help Molecular and Cellular Biology researchers in the use of these microorganisms to approach the study of general or specific key questions in cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Cytokinesis: Methods and Protocols provides practical and step-by-step detailed protocols useful for a wide audience ranging from experienced researchers to beginners in the use of yeasts.
Yeast Diversity in Human Welfare
by Tulasi Satyanarayana Gotthard KunzeThis book brings together and updates the latest information on the diversity of yeasts, their molecular features and their applications in the welfare of mankind. Yeasts are eukaryotic microfungi widely found in natural environments, including those with extreme conditions such as low temperatures, low oxygen levels and low water availability. To date, approximately 2,000 of the estimated 30,000 to 45,000 species of yeast on Earth, belonging to around 200 genera have been described. Although there are a few that are opportunistic human and animal pathogens, the vast majority of yeasts are beneficial, playing an important role in the food chain and in the carbon, nitrogen and sulphur cycles. In addition, yeasts such as Saccharomyces cerevisiae, Hansenula polymorpha and Pichia pastoris are used in expressing foreign genes to produce proteins of pharmaceutical interest. A landmark in biotechnology was reached in 1996 with the completion of sequencing of the entire S. cerevisiae genome, and it has now become a central player in the development of an entirely new approach to biological research and synthetic biology. The sequencing of genomes of several yeasts including Schizosaccharomyces pombe, Candida albicans and Cryptococcus neofromans has also recently been completed.
Yeast Functional Genomics: Methods and Protocols (Methods in Molecular Biology #2477)
by Frédéric DevauxThis second edition volume discusses the latest techniques and protocols used in the field that were not covered in the previous edition. The chapters in this book are organized into five parts. Part One looks at transcriptomic analyses and Part Two covers DNA replication and protein/DNA interactions. Part Three discusses translation dynamics, protein complexes, and proteomics. Part Four looks at genotypic screens and phenotypic profiling, and Part Five explores in silico integration of functional genomics data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and practical, Yeast Functional Genomics: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about the evolving field of yeast.Chapters 1, 9, 16, 20, 22, 24, and 25 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Yeast Functional Genomics and Proteomics
by Igor StagljarGiven the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. As part of the highly successful Methods in Molecular BiologyTM series, the chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Yeast Functional Genomics and Proteomics: Methods and Protocols is an ideal reference for both yeast researchers and those who wish to use yeast as a model system for the further study of functional genomics and proteomics.
Yeast Genetic Networks
by Attila BecskeiRegulation of gene expression, a major determinant of gene activity, occupies a central place in molecular biology. Yeast Genetic Networks: Methods and Protocols covers approaches to the systems biological analysis of small-scale gene networks in yeast. Divided into four convenient sections, this detailed volume discusses the methods used to analyze gene expression quantitatively, presents a collection of mathematical and computational tools to analyze stochasticity, adaptation, sensitivity in signal transmission, and oscillations in gene expression, provides instructional methods on how to utilize the tools of quantitative genetics to identify genes that regulate stochasticity and oscillations in gene expression, and concludes with a section devoted to the analysis of conserved gene expression systems and networks in different fungal species. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Yeast Genetic Networks: Methods and Protocols serves as an ideal and valuable resource for both novices and experienced researchers.
Yeast Genetics
by Jeffrey S. Smith Daniel J. BurkeYeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.
Yeast Membrane Transport
by Maik Kschischo Hana Sychrová José RamosThis contributed volume reviews the recent progress in our understanding of membrane transport in yeast including both Saccharomyces cerevisiae and non-conventional yeasts. The articles provide a summary of the key transport processes and put these in a systems biology context of cellular regulation, signal reception and homeostasis. After a general introduction, readers will find review articles covering the mechanisms and regulation of transport for various substrates ranging from diverse nutrients to cations, water and protons. These articles are complemented by a chapter on extremophilic yeast, a chapter on the mathematical modelling of ion transport and two chapters on the role of transport in pathogenic yeasts and antifungal drug resistance. Each article provides both a general overview of the main transport characteristics of a specific substrate or group of substrates and the unique details that only an expert working in the field is able to transmit to the reader. Researchers and students of the topic will find this book to be a useful resource for membrane transport in yeast collecting information in one complete volume, which is otherwise scattered across many papers. This might also be interesting for scientists investigating other species in order to compare transport mechanisms with known functions in yeast with the cells on which they work.
Yeast Metabolic Engineering
by Valeria MapelliYeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.
Yeast Metabolic Engineering: Methods and Protocols (Methods in Molecular Biology #2513)
by Valeria Mapelli Maurizio BettigaThis second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
Yeast Protocols
by Ivor H. EvansYeast Protocols contains many key techniques for studying the biology of yeasts at both the cellular and molecular levels. Working primarily from Saccharomyces cerevisiae, the expert contributors explain step-by-step how to successfully isolate, identify, and culture yeasts; the secrets of meiotic mapping; how to use PFGE in karyotyping and gene localization; the methods for purification and analysis of various cell components; and the construction and exploitation of genomic DNA clone banks. They also cover the latest methods for chromosome engineering, insertional mutagenesis by Ty elements, mRNA abundance and half-life measurements, the use of reporter gene systems, genotoxicity testing, and more. Yeast Protocols follows the widely applauded Humana Methods in Molecular Biology style: brief introductions putting the particular method in context, comprehensive lists of materials, cookbook style instructions, and troubleshooting notes to avoid common pitfalls and solve problems. The techniques can be used with confidence and success by both inexperienced newcomers and established researchers.
Yeast Protocols
by Wei XiaoIn this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.
Yeast Protocols (Methods in Molecular Biology #2196)
by Wei XiaoThis fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community.
Yeast Strain Selection
by Chandra J. PanchalContributors from universities and food, pharmaceutical, and brewing companies detail the current state of yeast strain development and handling, highlighting advances in yeast selection for academic research, industry, and recombinant DNA technology. Featuring the use of Saccharomyces and other yea
Yeast Surface Display
by Bin LiuIn addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers.
Yeast Surface Display (Methods in Molecular Biology #2491)
by Michael W. TraxlmayrThis detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.
Yeast Systems Biology: Methods and Protocols (Methods in Molecular Biology #2049)
by Juan I. Castrillo Stephen G. OliverThis second edition volume expands on the previous edition with a look at the latest advances in techniques to study yeast and its core set of interactions, modules, architectures, and network dynamics that are common in all eukaryotes. The chapters in this book are organized into Four Parts: Part One provides readers with an update on the development of novel experimental and computational approaches to yeast systems biology; Part Two explores high-throughput methods used to study yeast epigenome, transcriptome, proteome, and metabolome; Part Three talks about computational systems biology, and focuses on data management, dynamic modeling, constraint-based models of metabolic networks, and multi-level ‘omics data; while Part Four looks at experimental platforms that utilize yeast to model systemic human diseases such as Alzheimer’s and Parkinson’s diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Yeast System Biology: Methods and Protocols, Second Edition is a valuable tool for graduate students, post-doctoral researchers, and experts who are interested in learning about the latest developments in the study of yeast.
Yeast Systems Biology
by Juan I. Castrillo Stephen G. OliverSystems Biology aims at deciphering the genotype-phenotype relationships at the levels of genes, transcripts (RNAs), peptides, proteins, metabolites, and environmental factors participating in complex cellular networks in order to reveal the mechanisms and principles governing the behavior of complex biological systems. Yeast Systems Biology: Methods and Protocols presents an up-to-date view of the optimal characteristics of the yeast Saccharomyces cerevisiae as a model eukaryote, perspective on the latest experimental and computational techniques for systems biology studies, most of which were first designed for and validated in yeast, and selected examples of yeast systems biology studies and their applications in biotechnology and medicine. These experiments under controlled conditions can uncover the complexity and interplay of biological networks with their dynamics, basic principles of internal organization, and balanced orchestrated functions between organelles in direct interaction with the environment as well as the characterization of short and long-term effects of perturbations and dysregulation of networks that may illuminate the origin of complex human diseases. Written for the highly successful Methods in Molecular BiologyTM series, this volume contains the kind of detailed description and implementation advice that is crucial for getting optimal results. Practical and cutting-edge, Yeast Systems Biology: Methods and Protocols serves researchers interested in comprehensive systems biology strategies in well-defined model systems with specific objectives as well as a better knowledge of the latest post-genomic strategies at all 'omic levels and computational approaches towards analysis, integration, and modeling of biological systems, from single-celled organisms to higher eukaryotes.
Yeasts in the Production of Wine
by Patrizia Romano Maurizio Ciani Graham H. FleetIt is well established that certain strains of yeasts are suitable for transforming grape sugars into alcohol, while other yeast strains are not suitable for grape fermentations. Recent progress has clearly demonstrated that the sensory profile of a wine is characteristic of each vine cultivated, and the quality and technological characteristics of the final product varies considerably due to the strains which have performed and/or dominated the fermentation process. Because of their technological properties, wine yeast strains differ significantly in their fermentation performance and in their contribution to the final bouquet and quality of wine, such as useful enzymatic activities and production of secondary compounds related both to wine organoleptic quality and human health. The wine industry is greatly interested in wine yeast strains with a range of specialized properties, but as the expression of these properties differs with the type and style of wine to be made, the actual trend is in the use of selected strains, which are more appropriate to optimize grape quality. Additionally, wine quality can be influenced by the potential growth and activity of undesirable yeast species, considered spoilage yeasts, which cause sluggish and stuck fermentation and detrimental taste and aroma in the wine.
Yellow Biotechnology I
by Andreas VilcinskasUtility of Insects for Studying Human Pathogens and Evaluating New Antimicrobial Agents, by Yan Wang, De-Dong Li, Yuan-Ying Jiang and Eleftherios Mylonakis. Galleria Mellonella as a Model Host to Study Gut Microbe Homeostasis and Brain Infection by the Human Pathogen Listeria Monocytogenes, by Krishnendu Mukherjee, Ramya Raju, Rainer Fischer and Andreas Vilcinskas. Drosophila as a Model to Study Metabolic Disorders, by Julia Hoffmann, Renja Romey, Christine Fink and Thomas Roeder. The Fruit Fly Drosophila melanogaster as a Model for Aging Research, by Annely Brandt and Andreas Vilcinskas. Drosophila and the Hallmarks of Cancer, by Theodoulakis Christofi and Yiorgos Apidianakis. The red flour beetle Tribolium castaneum as a model to monitor food safety and functionality, by Stefanie Grünwald, Iris V. Adam, Ana-Maria Gurmai, Ludmila Bauer, Michael Boll, and Uwe Wenzel. Identification and Bioanalysis of Natural Products from Insect Symbionts and Pathogens, by Alexander O. Brachmann and Helge B. Bode. Antiparasitic Peptides, by Jette Pretzel, Franziska Mohring, Stefan Rahlfs and Katja Becker.
Yellow Perch, Walleye, and Sauger: Aspects of Ecology, Management, and Culture (Fish & Fisheries Series #41)
by John Clay Bruner Robin L. DeBruyneWalleye, one of the most sought-after species of freshwater sport fishes in North America, has demonstrated appreciable declines in their numbers from their original populations since the beginning of the 20th century. Similarly, Yellow Perch, once the most commonly caught sport fish and an important commercial species in North America, have also shown declines. Compiling up-to-date information on the biology and management of Walleye, Sauger, and Yellow Perch, including research on systematics, genetics, physiology, ecology, movement, population dynamics, culture, recent case histories, and management practices, will be of interest to managers, researchers, and students who deal with these important species, particularly in light of habitat alterations, population shifts, and other biotic and abiotic factors related to a changing climate.