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Adenovirus: Methods and Protocols (Methods in Molecular Biology #1089)
by Miguel Chillón Assumpció BoschReflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of "Adenovirus Methods and Protocols" calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, "Adenovirus Methods and Protocols, Third Edition" serves as an ideal guide for scientists continuing to research this highly valuable viral tool.
Adenovirus Methods and Protocols: Volume 2: Ad Proteins And Rna, Lifecycle And Host Interactions, And Phyologenetics (Methods in Molecular Medicine #21)
by William S. WoldIn Adenovirus Methods and Protocols, William S.M. Wold has organized a collection of readily reproducible methods for conducting research with adenoviruses, the premier and most widely used model in cell and molecular biology. The methods range from how to grow and titer adenoviruses and how to construct specific alterations in the adenovirus genome, to how to measure apoptosis induced by cells of the immune system, cytokines, and intrinsic apoptosis effectors. In addition, there are methods to study transcription and splicing with in vitro systems and for the adenovirus-mediated transformation of cells to a malignant state. Each method is written by a prominent investigator well-versed in the technique and includes a brief background discussion, as well as tried and true step-by-step instructions.<P><P> Adenovirus Methods and Protocols will be useful to both entry-level and senior scientists seeking to enter the adenovirus field, to researchers from other areas wishing to construct adenovirus vectors for their own research, and to adenovirologists wanting to enter new sectors of research. Its cutting-edge techniques are certain to make it today's reference of choice, one from which even expert researchers will learn many productive and time-saving techniques.
Adenovirus Methods and Protocols, Second Edition, Volume 1: Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models (Methods in Molecular Medicine #130)
by William S. Wold Ann E. TollefsonAdenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding the first edition, the authors have added new chapters that address innovative areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers.
Adenovirus Methods and Protocols, Second Edition, Volume 2: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics (Methods in Molecular Medicine #131)
by William S. Wold Ann E. TollefsonAdenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding the first edition, the authors have added new chapters that address innovative areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers.
ADHD as a Model of Brain-Behavior Relationships (SpringerBriefs in Neuroscience)
by Leonard F. Koziol Deborah Ely Budding Dana ChidekelADHD as a Model of Brain-Behavior Relationships Leonard F. Koziol, Deborah Ely Budding, and Dana Chidekel Series Title: Springer Briefs in Neuroscience Subseries: The Vertically Organized Brain in Theory and Practice It's been a basic neurological given: the brain does our thinking, and has evolved to do the thinking, as controlled by the neocortex. In this schema, all dysfunction can be traced to problems in the brain's lateral interactions. But in scientific reality, is this really true? Challenging this traditional cortico-centric view is a body of research emphasizing the role of the structures that control movement-the brain's vertical organization-in behavioral symptoms. Using a well-known, widely studied disorder as a test case, ADHD as a Model of Brain-Behavior Relationships offers an innovative framework for integrating neuroscience and behavioral research to refine diagnostic process and advance the understanding of disorders. Identifying a profound disconnect between current neuropsychological testing and the way the brain actually functions, this revision of the paradigm critiques the DSM and ICD in terms of the connectedness of brain structures regarding cognition and behavior. The authors argue for a large-scale brain network approach to pathology instead of the localizing that is so common historically, and for an alternate set of diagnostic criteria proposed by the NIMH. Included in the coverage: The diagnosis of ADHD: history and context. ADHD and neuropsychological nomenclature Research Domain Criteria: a dimensional approach to evaluating disorder The development of motor skills, executive function, and a relation to ADHD The role of the cerebellum in cognition, emotion, motivation, and dysfunction How large-scale brain networks interact Heralding a more accurate future of assessment, diagnosis, and treatment of neurodevelopmental disorders, ADHD as a Model of Brain-Behavior Relationships represents a major step forward for neuropsychologists, child psychologists, and psychiatrists, or any related profession interested in a neuroscientific understanding of brain function.
ADHD in Adults: A Practical Guide to Evaluation and Management (Current Clinical Psychiatry #0)
by Craig B.H. SurmanADHD in Adults: A Practical Guide to Evaluation and Management is the product of a unique collaboration of international specialists. This volume offers easy-to-read guidance, and includes checklists, rating scales and treatment planning tools. It was designed for a broad audience of caregivers working in diverse settings, including psychiatrists, social workers, primary care physicians, nurse specialists and psychologists. The authors are highly acclaimed clinicians, investigators and educators. They offer step-by-step guidance for implementation of best practices, drawing from clinical research and their experience treating thousands of patients. They cover diagnosis, treatment planning, and state-of-the-art application of pharmacology, psychotherapy, skill-building, family system and environmental interventions - for both simple and complex cases. The scales and worksheets in this Guide were developed to efficiently facilitate assessment and management. The Editor is an international leader in the field from the Clinical and Research Program in Adult ADHD at Massachusetts General Hospital (MGH) and Harvard Medical School, which has made pioneering and highly cited contributions to the understanding of ADHD. This Guide is a definitive, indispensable resource for all health providers who wish to optimize their approach to adult patients with ADHD.
ADHD, Stress, and Development (SpringerBriefs in Psychology)
by Petr Bob Jana KonicarovaThis first-of-its-kind volume revisits current findings on ADHD in terms of classic thinking on developmental neuropsychology for a more rounded concept of brain disorganization. Insights from Freud, Janet, John Hughlings Jackson, and other pioneers help identify mechanisms (e.g., the primitive reflexes) that can cause children with ADHD to be prone to cognitive dissociation when exposed to stressful environments. The authors’ model of the developing distracted brain pinpoints effects of stress on cognitive and affective functions, most notably attention and memory, and suggests situations in which stimuli may facilitate integration between brain and mind. This expanded knowledge opens out new educational possibilities for vulnerable students as well as new opportunities for therapeutic breakthroughs for children with ADHD. Included in the coverage: · Definition, diagnosis, and epidemiology of Attention Deficit and Hyperactivity Disorder. · Historical and recent research on ADHD. · Attentional functions, executive dysfunctions, and stress, implications for ADHD. · Neural dissolution, dissociation, and stress in ADHD. · Attention, brain-mind integration and ADHD. · Implications for education and therapy of ADHD children. ADHD, Stress, and Development ably synthesizes past and current understanding into a robust framework with implications for real-world practice. It offers practitioners and researchers new perspectives and future directions in neuropsychology, psychiatry, child and school psychology, and pediatrics.
Adherens Junctions: From Molecular Mechanisms To Tissue Development And Disease (Subcellular Biochemistry #60)
by Tony HarrisCell-cell adhesion is fundamental for the development and homeostasis of animal tissues and organs. Adherens junctions (AJs) are the best understood cell-cell adhesion complexes. In this volume, a group of internationally recognized experts reviews AJ biology over a wide range of organization; from atoms to molecules, to protein complexes, molecular networks, cells, tissues, and overall animal development. AJs have also been an integral part of animal evolution, and play central roles in cancer development, pathogen infection and other diseases. This book addresses major questions encompassing AJ biology. * How did AJs evolve? * How do cadherins and catenins interact to assemble AJs and mediate adhesion? * How do AJs interface with other cellular machinery to couple adhesion with the whole cell? * How do AJs affect cell behaviour and multicellular development? * How can abnormal AJ activity lead to disease?
Adhesion and Bonding in Composites
by Ryutoku YosomiyaThis book deals with the roles played by the component material interface in composites, with special emphasis on methods used to improve the adhesion and bonding between them. It is helpful for scientists and engineers in the materials field and to engineers working with adhesives or composites.
Adhesion in Layered Cement Composites
by Łukasz SadowskiThis book discusses how to identify the level of adhesion in layered systems made of cement composites using a multi-scale approach based on experimental and numerical analyses. In particular, it explains <p><p> 1. The suitability of previously used artificial intelligence tools and learning algorithms for reliable assessment of the level of adhesion of layered systems made of cement composites based on non-destructive tests <p> 2. The development of the methodology for a reliable non-destructive evaluation of the level of adhesion in newly constructed layered systems of any overlay thickness and in existing layered systems made of cement composites <p> 3. How to determine whether to assess the level of adhesion of the layered systems, and discusses the amplitude parameters, spatial, hybrid and volume parameters describing the morphology of the concrete substrate surface in the mesoscale <p> 4. How to ascertain whether the effective surface area of the existing concrete substrate and the contribution of the exposed aggregate on this substrate, determined in mesoscale, have an impact on the level of adhesion of layered systems made of cement composites <p> 5. The assessment of the structure of air pores in the microscale and the chemical composition of the cement composite on the nanoscale in the interphase zone together with the determination of their impact on the level of adhesion of layered systems made of cement composites <p> 6. The development of an effective methodology for testing the level of adhesion of layered systems made of cement composites in a multi-scale approach, including the research methods and descriptors used.
Adhesion Measurement Methods: Theory and Practice
by Robert LacombeAdhesion Measurement Methods: Theory and Practice provides practical information on the most important measurement techniques, their unique advantages and disadvantages, and the selection of the proper method for a given application. It includes useful information and formulae on adhesion related matters such as driving force formulae for various m
Adhesion Molecules in Allergic Disease
by Bruce S. BochnerThis unique resource-the first book of its kind exclusively devoted to the subject-offers timely coverage of the cells, mechanisms, and proteins involved in allergic inflammation, emphasizing the latest advances in local cell recruitment.
Adhesion of Cells, Viruses and Nanoparticles
by Kevin Kendall Florian Rehfeldt Michaela Kendall"Adhesion of Cells, Viruses and Nanoparticles" describes the adhesion of cells, viruses and nanoparticles starting from the basic principles of adhesion science, familiar to postgraduates, and leading on to recent research results. The underlying theory is that of van der Waals forces acting between cells and substrates, embodied in the molecules lying at the surfaces, together with the geometry and elasticity of the materials involved. The first part describes the fundamental background to adhesion principles, including the phenomenology, the important equations and the modeling ideas. Then the mechanisms of adhesion are explored in the second part, including the elastic deformations of spheres and the importance of the energy of adhesion as measured in various tests. It is demonstrated that adhesion of cells is statistical and depends on Brownian movement and on the complex multiple contacts that can form as cells move around. Then, detailed chapters on cell adhesion, contact of viruses and aggregation of nanoparticles follow in Part 3. Finally, the last chapter looks to the future understanding of cell adhesion and points out some interesting directions of research, development and treatment of diseases related to these phenomena. This book is an ideal resource for researchers on adhesion molecules, receptors, cell and tissue culturing, virus infection, toxicity of nanoparticles and bioreactor fouling. It can also be used to support undergraduate and Masters level teaching courses. "This is a fascinating book and it is an invaluable resource for understanding particle-particle/surface adhesion at micro- and nano- scales. I intend to keep one for my future reference and highly recommend it to my students." (Prof. Zhibing Zhang, School of Chemical Engineering, University of Birmingham, UK)
Adhesion Protein Protocols (Methods in Molecular Biology #96)
by Monica Corada Elisabetta DejanaAn international corps of expert investigators describe their optimized techniques for both the identification of new cell adhesion proteins and for the characterization of novel adhesive structures. Their cutting edge methods will enable both novice and experienced researchers readily to identify and clone new adhesion proteins, to successfully produce inhibitors of the adhesive activity, to develop biological models for the assay of cell-to-matrix and cell-to-cell adhesion in vitro, and to measure the signaling activities of adhesion proteins. By illuminating these adhesive molecules and the possibilities for manipulating them, the new experimental strategies collected here will have considerable clinical potential for the regulation of immunity, inflammation, tissue remodeling, and embryonic development.
Adhesion Protein Protocols (Methods in Molecular Biology #1046)
by Amanda S. CouttsThe second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.
Adhesive Bonding in Five Steps: Achieving Safe and High-Quality Bonds
by Jürgen KlingenA comprehensive guide to successfully complete any adhesive bonding project In Adhesive Bonding in Five Steps: Achieving Safe and High Quality Bonds, accomplished chemist Dr. Jürgen Klingen delivers a thorough and practical overview of the adhesive concepts necessary for readers to design and sketch foundational steps in projects involving adhesive bonding. Readers will learn the complex considerations necessary for a successful adhesive bonding process from inception to completion. Dividing the adhesive bonding project into five clearly defined phases—planning, substrate concept, adhesive concept, feasibility, and development—the author demonstrates how to adhere to quality requirements while completing the reader’s own adhesive bonding processes. The book focuses on the treatment of the material surfaces to be bonded, the selection of suitable adhesives, the dimensioning of the bond, and the process steps for metering/mixing and curing the adhesives. The book also offers: A thorough introduction to the art and science of adhesive bonding, including adhesives, adhesive bonds, bonding in industry and craft, and an example of adhesive bonding in nature Comprehensive explorations of the history of adhesive bonding technology and wetting, adhesion, and cohesion Practical discussions of the necessary steps to achieving safe and high-quality adhesive bonds, including gate reviews and the DIN 2304 In-depth examinations of contemporary adhesive bonding applications, including examples in lightweight construction, modern façade construction, and low-energy plastics Perfect for engineers, engineering scientists, polymer chemists, and process engineers, Adhesive Bonding in Five Steps: Achieving Safe and High Quality Bonds will also prove to be an invaluable addition to the libraries of materials scientists and surface chemists.
Adhesive Bonding Technology and Testing
by Ana Sofia Queiros Ferreira Barbosa Lucas Filipe da Silva Ricardo Joao Camilo Carbas Eduardo Andre Sousa Marques Alireza Akhavan-SafarAdhesive Bonding Technology and Testing Comprehensive resource that provides insight into the purpose and design of experiments for adhesive bonding, joint design and strength prediction This book provides support for those practicing and teaching adhesive bonding and enables them to understand and design laboratorial courses and experiments. To aid in reader comprehension and information retention, a selected set of problems with corresponding solutions is included, which helps readers to develop a deep understanding of the subject matter. Written by five highly qualified professionals in the field of adhesive bonding, sample topics covered in the book include: Practical demonstrations of adhesive bonding, plus discussion on the advantages and disadvantages of the technique Detailed laboratorial activities that pertain to adhesive bonding The manufacturing of defect-free bonded joints The effects of geometry and materials properties in adhesive joint testing, surface preparation, joint design, and strength prediction This book is an essential resource for chemists, engineers, and students/instructors in related programs of study who wish to conduct better and more efficient experiments that pertain to adhesive bonding and related concepts.
Adhesive Interactions in Normal and Transformed Cells
by Yury A. RovenskyAdhesive Interactions in Normal and Transformed Cells describes the basic mechanisms of the ability of tissue cells to attach to each other and to the extracellular matrix. These adhesive interactions are pivotal regulators of main cellular functions, such as proliferation, survival and migration. The adhesive interactions are involved in embryonic development, regeneration, and also in inflammation and degeneration processes, which are at the basis of many diseases. Serious alterations in cell adhesion caused by the oncogenic transformation play a key role in cancer invasion and metastasis. This volume provides comprehensive information about structural, mechanistic and signaling aspects of adhesive interactions in both normal and cancer cells in comparison. Integration of such aspects of the adhesive process as structure, relation to cell systems of receptors and cytoskeleton, function, signaling pathways, and the alterations in tumor cells constitutes the strongest point of this work. The results of the long-time author's research are included in the book. The author was one of pioneers, who used scanning electron microscopy (SEM) to study the cell surface morphology of normal cultured cells and the cells underwent the oncogenic transformation, processes of their attachment to and spreading on the surfaces of a solid substratum, and also surprising ability of the cells to respond to various geometric configurations of the substrata surfaces. Adhesive Interactions in Normal and Transformed Cells has both biological and medical aspects and, therefore, it can be interesting not only for cell biologists, developmental biologists and cancer researchers, but also for physicians. It is intended for researchers, postdocs, undergraduate and graduate students.
Adhesive Interactions of Mussel Foot Proteins (Springer Theses)
by Jing YuWater and moisture undermine strong adhesion to polar surfaces. Marine mussels, however, achieve durable underwater adhesion using a suite of proteins that are peculiar in having high levels of 3, 4-dihydroxyphenylalanine (Dopa). Mussel adhesion has inspired numerous studies on developing the next generation of wet adhesives. This thesis presents recent progress in understanding the basic surface and intermolecular interactions employed by mussels to achieve strong and durable wet adhesion. The surface forces apparatus (SFA) and various other techniques were applied to measure the interactions between mussel foot protein-3 fast (Mfp-3 fast) and the model substrate, mica, as well as the interactions between various mussel adhesive proteins The results in this thesis show that Dopa plays an essential role in mussel adhesion and that mussels delicately control the interfacial redox environment to achieve strong and durable Dopa mediated adhesion. The interplay between Dopa and hydrophobic interactions is also evident in mussel adhesion.
Adhesive Joints: Volume 2
by K. L. MittalThis volume documents the proceedings of the Second International Symposium on Adhesive Joints: Formation, Characteristics and Testing held in Newark, NJ, May 22-24, 2000. Since the first symposium, held in 1982, there had been tremendous research activity dealing with many aspects of adhesive joints.This volume contains a total of 21 papers, which were all properly peer reviewed, revised and edited before inclusion. Therefore, this book is not merely a collection of unreviewed manuscripts, but rather represents information which has passed peer scrutiny. Furthermore, the authors were asked to update their manuscripts, so the information contained in this book should be current and fresh.The book is divided into three parts: 1) General Papers; 2) Evaluation, Analysis and Testing; and 3) Durability Aspects. The topics covered include: molecular brush concepts in enhancing strength of adhesive joints; factors affecting performance of adhesive joints; substrate preparation and modification; interfacial/interphasial aspects; determination of locus of failure; analysis and evaluation of adhesive joints using various techniques; testing of adhesive joints; stress analysis; application of fracture mechanics; durability aspects; accelerated environmental degradation of adhesive joints; solvent uptake; and adhesives with special characteristics.This volume represents a commentary on the current R&D activity in this arena and it should be of great value and interest to anyone interested in adhesive bonding / adhesive joints. Furthermore, this volume contains a number of excellent review/overview articles, which should be of particular value.
Adhesive Particle Flow
by Jeffrey S. Marshall Shuiqing LiOffering a comprehensive treatment of adhesive particle flows, this book adopts a particle-level approach oriented toward directly simulating the various fluid, electric field, collision, and adhesion forces and torques acting on the particles, within the framework of a discrete-element model. It is ideal for professionals and graduate students working in engineering and atmospheric and condensed matter physics, materials science, environmental science, and other disciplines where particulate flows have a significant role. The presentation is applicable to a wide range of flow fields, including aerosols, colloids, fluidized beds, and granular flows. It describes both physical models of the various forces and torques on the particles as well as practical aspects necessary for efficient implementation of these models in a computational framework.
Adhesives and Finishes for Wood: For Practitioners and Students
by Moon G. KimAdhesives and Finishes for Wood Understand the science of joining wood with this comprehensive guide Long seen as an old-fashioned material with narrowing modern applications, wood has seen increased popularity as a material in building and manufacturing in recent years. This has been driven by the need for sustainable resources and environmentally friendly materials. As a result of increased emphasis on wood, however, there is a corresponding need to understand the wood adhesives, the crucial materials in wood-based manufacture and craftsmanship. Adhesives and Finishes for Wood meets this need with a comprehensive but accessible introduction to the chemistry and applications of wood adhesives. Its easy-to-follow presentation nonetheless presents wood adhesives and finishes in significant detail. Ideal for readers without considerable preexisting knowledge in chemistry, this book includes everything the reader needs to understand and apply wood adhesives in their work or industry. Adhesives and Finishes for Wood readers will also find: Coverage ranging from the fundamentals of wood adhesive polymer chemistry to the properties of specific wood structures and resins A presentation suitable for both academic students and wood manufacture professionals An author with decades of experience in both academia and industry Adhesives and Finishes for Wood is a useful reference for advanced students and professionals in industries or manufacturing disciplines that incorporate wood, as well as for chemists, materials scientists, vocational school instructors, and more.
The ADI Model Problem
by Eugene WachspressThe ADI Model Problem presents the theoretical foundations of Alternating Direction Implicit (ADI) iteration for systems with both real and complex spectra and extends early work for real spectra into the complex plane with methods for computing optimum iteration parameters for both one and two variable problems. This book provides application of theory to the solution of boundary value problems and description of stable similarity reduction of a full matrix to low-band upper Hessenberg form, with application to computation of eigenvalues and solution of Lyapunov and Sylvester equations. Also included are MATLAB programs and numerical verification of theory and applications.
Adipose-Derived Stem Cells: Methods And Protocols (Methods In Molecular Biology #702)
by Bruce A. Bunnell Jeffrey M. GimbleDuring the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology™ series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.
Adipose-Derived Stem Cells: Methods and Protocols (Methods in Molecular Biology #702)
by Bruce A. Bunnell Jeffrey M. GimbleDuring the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular BiologyTM series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.