- Table View
- List View
DNA Methods in Food Safety
by Omar A. Oyarzabal Sophia KathariouMolecular typing of foodborne pathogens has become an indispensable tool in epidemiological studies. Thanks to these techniques, we now have a better understanding of the distribution and appearance of bacterial foodborne diseases and have a deeper knowledge of the type of food products associated with the major foodborne pathogens. Within the molecular techniques, DNA-based techniques have prospered for more than 40 years and have been incorporated in the first surveillance systems to monitor bacterial foodborne pathogens in the United States and other countries. However, DNA techniques vary widely and many microbiology laboratory personnel working with food and/or water face the dilemma of which method to incorporate.DNA Methods in Food Safety: Molecular Typing of Foodborne and Waterborne Bacterial Pathogens succinctly reviews more than 25 years of data on a variety of DNA typing techniques, summarizing the different mathematical models for analysis and interpretation of results, and detailing their efficacy in typing different foodborne and waterborne bacterial pathogens, such as Campylobacter, Clostridium perfringens, Listeria, Salmonella, among others. Section I describes the different DNA techniques used in the typing of bacterial foodborne pathogens, whilst Section II deals with the application of these techniques to type the most important bacterial foodborne pathogens. In Section II the emphasis is placed on the pathogen, and each chapter describes some of the most appropriate techniques for typing each bacterial pathogen.The techniques presented in this book are the most significant in the study of the molecular epidemiology of bacterial foodborne pathogens to date. It therefore provides a unique reference for students and professionals in the field of microbiology, food and water safety and epidemiology and molecular epidemiology.
DNA Modification Detection Methods (Springer Protocols Handbooks)
by Bi-Feng YuanUnderstanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years. This book provides a comprehensive overview of available techniques and methods together with detailed step-by-step protocols for experimental procedures required to successfully perform analysis on various types of DNA modifications, including 5mC, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU), 5-formyluracil (5fU), N6-methyladenine (6mA), β-D-glucosyl-5-hydroxymethyluracil (base J) and 8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable source for biochemists and molecular biologists from different fields who wish to investigate DNA modifications.
DNA Nanoscience: From Prebiotic Origins to Emerging Nanotechnology
by Kenneth DouglasDNA Nanoscience: From Prebiotic Origins to Emerging Nanotechnology melds two tales of DNA. One is a look at the first 35 years of DNA nanotechnology to better appreciate what lies ahead in this emerging field. The other story looks back 4 billion years to the possible origins of DNA which are shrouded in mystery. The book is divided into three parts comprised of 15 chapters and two Brief Interludes. Part I includes subjects underpinning the book such as a primer on DNA, the broader discipline of nanoscience, and experimental tools used by the principals in the narrative. Part II examines the field of structural DNA nanotechnology, founded by biochemist/crystallographer Nadrian Seeman, that uses DNA as a construction material for nanoscale structures and devices, rather than as a genetic material. Part III looks at the work of physicists Noel Clark and Tommaso Bellini who found that short DNA (nanoDNA) forms liquid crystals that act as a structural gatekeeper, orchestrating a series of self-assembly processes using nanoDNA. This led to an explanation of the polymeric structure of DNA and of how life may have emerged from the prebiotic clutter.
DNA Nanotechnology
by Chunhai FanDNA nanotechnology: From structure to function presents an overview of various facets of DNA nanotechnology, with a particular focus on their promising applications. This book is composed of three parts. Part I, Elements of DNA Nanotechnology, provides extensive basic information on DNA nanotechnology. Part II, Static and Dynamic DNA Nanotechnology, describes the design and fabrication of static and dynamic DNA nanostructures. Recent advances in DNA origami, DNA walkers and DNA nanodevices are all covered in this part. Part III, Applications of DNA Nanotechnology, introduces a variety of applications of DNA nanotechnology, including biosensing, computation, drug delivery, etc. Together these provide a comprehensive overview of this emerging area and its broad impact on biological and medical sciences. This book is intended for post-graduates, post-doctoral researchers and research scientists who are interested in expanding their knowledge of DNA nanotechnology. It provides readers an impression of the latest developments in this exciting filed.
DNA Nanotechnology
by Bruno Samorì Giampaolo ZuccheriCurrently, nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the biological behavior and function of DNA. With the structural perfection of a self-assembling DNA nano-object, such as a DNA origami, it is clear how complex DNA is as a molecule, leading researchers to wonder how many different constructs could be designed and realized. DNA Nanotechnology: Methods and Protocols shows the procedures to follow in order to repeat methods that lead to such constructs or to the mastering of the characterization techniques used to study them. The chapters of this book are roughly divided into two parts: some cover the methods for preparing the nanostructures, from the rationale of the operations to the techniques for their handling, while other chapters deal more directly with advanced instrumental techniques that can manipulate and characterize molecules and nanostructures. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, DNA Nanotechnology: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to ignite interest and spur activity in this young and rapidly growing research field. Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
DNA Nanotechnology for Cell Research: From Bioanalysis to Biomedicine
by Zhou NieDNA Nanotechnology for Cell Research Comprehensive coverage of DNA nanotechnology with a focus on its biomedical applications in disease diagnosis, gene therapy, and drug delivery Bringing together multidisciplinary aspects of chemical, material, and biological engineering, DNA Nanotechnology for Cell Research: From Bioanalysis to Biomedicine presents an overview of DNA nanotechnology with emphasis on a variety of different applications in cell research and engineering, covering a unique collection of DNA nanotechnology for fundamental research and engineering of living cells, mostly in cellulo and in vivo, for the first time. Broad coverage of this book ranges from pioneering concepts of DNA nanotechnology to cutting-edge reports regarding the use of DNA nanotechnology for fundamental cell science and related biomedical engineering applications in sensing, bioimaging, cell manipulation, gene therapy, and drug delivery. The text is divided into four parts. Part I surveys the progress of functional DNA nanotechnology tools for cellular recognition. Part II illustrates the use of DNA-based biochemical sensors to monitor and image intracellular molecules and processes. Part III examines the use of DNA to regulate biological functions of individual cells. Part IV elucidates the use of DNA nanotechnology for cell-targeted medical applications. Sample topics covered in DNA Nanotechnology for Cell Research include: Selections and applications of functional nucleic acid toolkits, including DNA/RNA aptamers, DNAzymes, and riboswitches, for cellular recognition, metabolite detection, and liquid biopsy. Developing intelligent DNA nanodevices implemented in living cells for amplified cell imaging, smart intracellular sensing, and in cellulo programmable biocomputing. Harnessing dynamic DNA nanotechnology for non-genetic cell membrane engineering, receptor signaling reprogramming, and cellular behavior regulation. Construction of biocompatible nucleic acid nanostructures as precisely controlled vehicles for drug delivery, immunotherapy, and tissue engineering. Providing an up-to-date tutorial style overview along with a highly valuable in-depth perspective, DNA Nanotechnology for Cell Research is an essential resource for the entire DNA-based nanotechnology community, including analytical chemists, biochemists, materials scientists, and bioengineers.
DNA Origami: Structures, Technology, and Applications
by Masayuki EndoDNA ORIGAMI Discover the impact and multidisciplinary applications of this subfield of DNA nanotechnology DNA origami refers to the technique of assembling single-stranded DNA template molecules into target two- and three-dimensional shapes at the nanoscale. This is accomplished by annealing templates with hundreds of DNA strands and then binding them through the specific base-pairing of complementary bases. The inherent properties of these DNA molecules—molecular recognition, self-assembly, programmability, and structural predictability—has given rise to intriguing applications from drug delivery systems to uses in circuitry in plasmonic devices. The first book to examine this important subfield, DNA Origami brings together leading experts from all fields to explain the current state and future directions of this cutting-edge avenue of study. The book begins by providing a detailed examination of structural design and assembly systems and their applications. As DNA origami technology is growing in popularity in the disciplines of chemistry, materials science, physics, biophysics, biology, and medicine, interdisciplinary studies are classified and discussed in detail. In particular, the book focuses on DNA origami used for creating new functional materials (combining chemistry and materials science; DNA origami for single-molecule analysis and measurements (as applied in physics and biophysics); and DNA origami for biological detection, diagnosis and therapeutics (medical and biological applications). DNA Origami readers will also find: A complete guide for newcomers that brings together fundamental and developmental aspects of DNA origami technology Contributions by a leading team of experts that bring expert views from different angles of the structural developments and applications of DNA origami An emerging and impactful research topic that will be of interest in numerous multidisciplinary areas A helpful list of references provided at the end of each chapter to give avenues for further study Given the wide scope found in this groundbreaking work, DNA Origami is a perfect resource for nanotechnologists, biologists, biophysicists, chemists, materials scientists, medical scientists, and pharmaceutical researchers.
DNA Replication
by Jacob Z. Dalgaard Sonya VengrovaSince the discovery of DNA structure and throughout the ensuing "DNA era", the field of DNA replication has expanded to cover a vast number of experimental systems. In DNA Replication: Methods and Protocols, expert researchers present a collection of techniques and approaches used to investigate DNA replication with an emphasis on the most recent technological developments. Beginning with several informative introductory review chapters, this extensive volume is organized for clarity while fully encouraging innovation by the mixing of methods to create new techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Replication: Methods and Protocols provides an excellent tool for both established laboratories and individuals new to this exciting field of research.
DNS of Wall-Bounded Turbulent Flows: A First Principle Approach
by Tapan K. Sengupta Swagata BhaumikThis book highlights by careful documentation of developments what led to tracking the growth of deterministic disturbances inside the shear layer from receptivity to fully developed turbulent flow stages. Associated theoretical and numerical developments are addressed from basic level so that an uninitiated reader can also follow the materials which lead to the solution of a long-standing problem. Solving Navier-Stokes equation by direct numerical simulation (DNS) from the first principle has been considered as one of the most challenging problems of understanding what causes transition to turbulence. Therefore, this book is a very useful addition to advanced CFD and advanced fluid mechanics courses.
DNS Security Management
by Michael Dooley Timothy RooneyAn advanced Domain Name System (DNS) security resource that explores the operation of DNS, its vulnerabilities, basic security approaches, and mitigation strategies DNS Security Management offers an overall role-based security approach and discusses the various threats to the Domain Name Systems (DNS). This vital resource is filled with proven strategies for detecting and mitigating these all too frequent threats. The authors—noted experts on the topic—offer an introduction to the role of DNS and explore the operation of DNS. They cover a myriad of DNS vulnerabilities and include preventative strategies that can be implemented. Comprehensive in scope, the text shows how to secure DNS resolution with the Domain Name System Security Extensions (DNSSEC). In addition, the text includes discussions on security applications facility by DNS, such as anti-spam, SPF, DANE and related CERT/SSHFP records. This important resource: Presents security approaches for the various types of DNS deployments by role (e.g., recursive vs. authoritative) Discusses DNS resolvers including host access protections, DHCP configurations and DNS recursive server IPs Examines DNS data collection, data analytics, and detection strategies With cyber attacks ever on the rise worldwide, DNS Security Management offers network engineers a much-needed resource that provides a clear understanding of the threats to networks in order to mitigate the risks and assess the strategies to defend against threats.
Do It Smart
by Jurgen Ringback Rolf-Dieter KempisA decade ago, manufacturing companies had visions of paperless offices, automated plants, and virtual enterprises. But the euphoria quickly evaporated when these visions failed to materialize. Now, from in-depth interviews in a worldwide survey of seventy manufacturing firms, a research team from the prestigious consulting group McKinsey & Company concludes that, far from being a failure, information technology (IT) can be a vital strategic weapon in the manufacturing sector, just as it has proved to be in service industries. In Do IT Smart, experts Rolf-Dieter Kempis and Jürgen Ringbeck along with the McKinsey team identify four cultures of IT users -- stars, big spenders, cautious spenders, and laggards -- based on how efficiently and effectively the users manage IT. The stars stand out because their strong command of IT means they are better able to manage core processes such as R&D, sales and service, and order processing, which in turn produces tangible payoffs in profitability, growth, and market share. From their study of star performers, the authors formulate seven rules for developing a superior IT organization. First, they argue, managers must make IT a top management issue and, second, a priority in product development. IT must be viewed as a strategic tool so that IT strategy can be aligned with business strategy. Clear objectives must be set, and core business processes redesigned. Warning that IT is reaching saturation in administrative applications, the authors describe how it is far more profitable to integrate IT into marketing, sales, and customer service. Finally, they describe how all these elements must be brought together into a lean, customer-oriented IT network. McKinsey's breakthrough study shows that as organizations are increasingly overwhelmed with data, IT will become more of a dividing line between the winners and the losers. IT stars will make quantum leaps in effectiveness, while poor management of IT results in a cost explosion. Managers and information officers who want their business to keep and gain the competitive edge IT offers need this unprecedented insight into how to Do IT Smart.
Do No Harm: Protecting Connected Medical Devices, Healthcare, and Data from Hackers and Adversarial Nation States
by Matthew WebsterDiscover the security risks that accompany the widespread adoption of new medical devices and how to mitigate them In Do No Harm: Protecting Connected Medical Devices, Healthcare, and Data from Hackers and Adversarial Nation States, cybersecurity expert Matthew Webster delivers an insightful synthesis of the health benefits of the Internet of Medical Things (IoMT), the evolution of security risks that have accompanied the growth of those devices, and practical steps we can take to protect ourselves, our data, and our hospitals from harm. You’ll learn how the high barriers to entry for innovation in the field of healthcare are impeding necessary change and how innovation accessibility must be balanced against regulatory compliance and privacy to ensure safety. In this important book, the author describes: The increasing expansion of medical devices and the dark side of the high demand for medical devices The medical device regulatory landscape and the dilemmas hospitals find themselves in with respect medical devices Practical steps that individuals and businesses can take to encourage the adoption of safe and helpful medical devices or mitigate the risk of having insecure medical devices How to help individuals determine the difference between protected health information and the information from health devices--and protecting your data How to protect your health information from cell phones and applications that may push the boundaries of personal privacy Why cybercriminals can act with relative impunity against hospitals and other organizations Perfect for healthcare professionals, system administrators, and medical device researchers and developers, Do No Harm is an indispensable resource for anyone interested in the intersection of patient privacy, cybersecurity, and the world of Internet of Medical Things.
Do Robots Make Love?: From AI to Immortality – Understanding Transhumanism in 12 Questions
by Laurent Alexandre Jean-Michel BesnierShould we enhance the human condition with technology?Does anyone really want to live for a thousand years?Could AI end up destroying mankind?Discover the incredible potential of mankind's near future as Doctor and entrepreneur Laurent Alexandre and tech-philosopher Jean Michel Besnier go head to head on the big questions in an entertaining and thought-provoking debate on the fundamental principles of transhumanism.This movement seeks to improve the human condition through science - has fast become one of the most controversial the scientific community have ever faced. As great strides are made in using advanced technology to enhance human intellect and physiology, the ethical and moral questions surrounding its possibilities have never been more pressing. Should we change the way we reproduce? Could we enhance the human body with technology to the point where we are all technically cyborgs? Is it possible to make love to a robot?
Do the Gulf Oil-Producing Countries Influence Regional Growth? The Impact of Financial and Remittance Flows
by Nadeem Ilahi Riham ShendyA report from the International Monetary Fund.
Do Unto Animals: A Friendly Guide to How Animals Live, and How We Can Make Their Lives Better
by Tracey Stewart Lisel Ashlock#1 New York Times bestseller and USA Today bestseller The more we know about the animals in our world and the better we care for them, the better our lives will be. Former veterinary technician and animal advocate Tracey Stewart understands this better than most—and she’s on a mission to change how we interact with animals. Through hundreds of charming illustrations, a few homemade projects, and her humorous, knowledgeable voice, Stewart provides insight into the secret lives of animals and the kindest ways to live with and alongside them. At home, she shows readers how to speak “dog-ese” and “cat-ese” and how to “virtually adopt” an animal. In the backyard, we learn about building bee houses, dealing nicely with pesky moles, and creative ways to bird-watch. And on the farm, Stewart teaches us what we can do to help all farm animals lead a better life (and reveals pigs’ superpowers!). Part practical guide, part memoir of her life with animals, and part testament to the power of giving back, Do Unto Animals is a gift for animal lovers of all stripes.
Do We Battle Like It's Medieval Times?: Military Technology Then and Now (Medieval Tech Today)
by Megan Cooley PetersonFrom gunpowder to armor, medieval innovators helped develop and improve some important weaponry and warfare technology we use today. The Middle Ages were crucial for the development of technologies such as warships, battering rams, cannons, and more! Discover how we still fight like we're in medieval times with interesting historical facts, scientific details, and illuminating photos.
Do We Build Like It's Medieval Times?: Construction Technology Then and Now (Medieval Tech Today)
by Megan Cooley PetersonFrom cranes to castles, medieval innovators helped develop and improve some important construction technology we use today. The Middle Ages were crucial for the development of technologies such as the chimney, central heating, the flying buttress, and more! Discover how we still build like we're in medieval times with interesting historical facts, scientific details, and illuminating photos.
Do You Live Like It's Medieval Times?: Personal Technology Then and Now (Medieval Tech Today)
by Megan Cooley PetersonFrom soap to pretzels, medieval innovators helped develop or improve some important technology we use in our daily lives. The Middle Ages were crucial for the improvement of chess, the compass, glasses, and more! Discover how we still live like we're in medieval times with historical facts, scientific details, and illuminating photos.
Do You See What I See? Memoirs of a Blind Biker
by Russell TargTarg has been visually handicapped since childhood and yet he has performed groundbreaking research in lasers and optics. This book provides the remarkable story of a visually impaired physicist who sees beyond perception to help readers find meaning and joy.
Do Your Ears Pop in Space and 500 Other Surprising Questions About Space Travel
by R. Mike Mullane"An excellent reference. This book has to be on the shelf of every space buff. " --James Lovell, Commander, Apollo 13. Get the inside story on outer space from three-time shuttle astronaut R. Mike Mullane. "A fascinating collection of honest, factual, from-the-heart answers to the most often asked questions about spaceflight and spacefliers. Required reading for all who aspire to travel in space. " --Kathy Thornton, 4-mission Shuttle Astronaut, World Record Holder for Spacewalks by a Woman. "A brilliant addition to the understanding of space flight. Only a man who has been there--outer space--and done that--fly the Space Shuttle--could render the complexities of flying in space so lucidly. " --Walter J. Boyne, Colonel, USAF (Ret. ), Former Director, National Air and Space Museum. "A highly informative inside view of what astronauts really experience in space. " --Ed Buckbee, Former Director, U. S. Space & Rocket and U. S. Space Camp. "All astronauts have been peppered with great questions. Mike Mullane has great answers. " --Vice Admiral Richard H. Truly, U. S. Navy (Ret. ), Columbia 1981, Challenger 1983, NASA Administrator 1989-1992.
Doctor Robot-O
by Lela NargiCan you imagine visiting a doctor's office for an illness or injury, but getting treated by a robot instead? According to some researchers, this could be the reality in the not too distant future. Ongoing research into artificial intelligence and its use in medicine might someday produce robots that will work alongside human doctors and nurses to deliver the best health care possible.
Doctor Venom
by Bryan Grieg FryMeet venomologist Bryan Grieg Fry, the man with one of the most dangerous job in Australia - working with the world's most deadly creatures.Welcome to the strange and dangerous world of Doctor Venom.Imagine a first date involving three weeks in Siberia catching venomous water shrews, and later a wedding attended by Eastern European prime ministers and their bodyguards wielding machine guns. Then a life of living and working with snakes. Lots of very, very poisonous snakes and other venomous creatures ... everything from the Malaysian king cobra to deadly scorpions.In this action-packed ride through Bryan Grieg Fry's life you'll meet the man who's worked with the world's most venomous creatures in over 50 countries. He's been bitten by 26 poisonous snakes and three stingrays - and, while deep in the Amazon jungle, survived a near-fatal scorpion sting. He's also broken 23 bones, including breaking his back in three places, and had to learn how to walk again. He only works on venom that he has collected himself - so the adventures, and danger, will just keep coming...Bryan now divides his time between scientific research and teaching at the University of Queensland, and TV filming and collecting expeditions around the world.
Doctor Who: a 1980s story
by Mark Griffiths Doctor Who*Part of the six books for six decades collection*Midnight, 1984.In a sprawling, run-down housing estate in south London, a man returning from a night out in the West End finds himself pursued by a strange hooded figure.So naturally when the Doctor and Romana arrive in the TARDIS the next day, they find themselves in the middle of a crime scene.But when child genius Matthew Pickles - inventor of a hugely popular handheld videogame - arrives to help them crack the case, they discover there is more to this than meets the eye.Someone has been messing with technology that's not of this earth, blurring the lines between human . . . and cyber. And it looks like they're out for revenge.In a world on the brink of gadgets and gismos and dangerous tech, the pair must uncover the killer, before they strike again.
Doctoral Symposium on Information and Communication Technologies: Second Doctoral Symposium, DSICT 2022, Manta, Ecuador, October 12–14, 2022, Proceedings (Communications in Computer and Information Science #1647)
by Karina Abad Santiago BerrezuetaThis book constitutes the refereed proceedings of the Second Doctoral Symposium on Information and Communication Technologies, DSICT 2022, held in Manta, Ecuador, in October 2022. The 15 full papers were thoroughly reviewed and selected from the 72 submissions. The papers present research in areas of intelligent systems, artificial intelligence, ICTs and their applications to the real world.
Doctoral Symposium on Information and Communication Technologies - DSICT (Lecture Notes in Electrical Engineering #846)
by Santiago Berrezueta Karina AbadInformation and communication technologies have provided great advances in fields such as medicine, industry, telecommunications, education, environmental protection, and more. The first edition of DSICT presents researches, advances and new challenges for ICTs in the above-mentioned fields through a collection of selected articles. All these contributions have been presented during the Doctoral Symposium on Information and Communication Technologies that brought together experts from various parts of the world to discuss and share what will be the starting points for new lines of research and working groups in the field of ICT. Professionals and researchers in the field of ICT will find in this book significant contributions to their research. Because of the breadth of the application of ICT, this book will also be useful for businessmen and entrepreneurs in the field of technology. They will be able to learn about the latest ICT applications and their future projections.