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Chromatin Immunoprecipitation: Methods and Protocols (Methods in Molecular Biology #2846)
by Franziska GreulichThis comprehensive guide delves into the protocols and strategies for investigating gene regulation both experimentally and computationally. It focuses on the use of Chromatin Immunoprecipitation (ChIP) coupled with next-generation sequencing, providing a robust framework for profiling DNA-binding proteins, examining DNA footprinting, and analyzing histone modifications. As part of the highly successful Methods in Molecular Biology series, this book offers introductions on the topics covered, detailed lists of the necessary materials and reagents, step-by-step laboratory protocols that are easy to follow, and expert tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Chromatin Immunoprecipitation: Methods and Protocols is the perfect resource for the new wave of genome biologists looking to master ChIP techniques.
Putting the EYFS Curriculum into Practice
by Julian Grenier Caroline VollansHow should practitioners and leaders set about designing and implementing their curriculum in the Early Years Foundation Stage? Written by experts in the field, this book provides clear, practical guidance on each of the 7 areas of learning and development in the revised (2021) EYFS. Chapters cover key topics including, assessment, inclusion of children with SEND, English as an Additional Language, equalities, anti-racism, provision for disadvantaged children, professional development and using research and evidence. Designed for practising teachers, early years educators, students on early years degrees and trainee teachers, this engaging book provides an accessible guide to putting revised EYFS into practice. Dr Julian Grenier CBE is the headteacher of Sheringham Nursery School and Children’s Centre. He was the lead writer of Development Matters (2021). Caroline Vollans is a psychoanalyst and author. She writes for a wide range of early years publications and is author of Wise Words: How Susan Isaacs Changed Parenting.
Putting the EYFS Curriculum into Practice
by Julian Grenier Caroline VollansHow should practitioners and leaders set about designing and implementing their curriculum in the Early Years Foundation Stage? Written by experts in the field, this book provides clear, practical guidance on each of the 7 areas of learning and development in the revised (2021) EYFS. Chapters cover key topics including, assessment, inclusion of children with SEND, English as an Additional Language, equalities, anti-racism, provision for disadvantaged children, professional development and using research and evidence. Designed for practising teachers, early years educators, students on early years degrees and trainee teachers, this engaging book provides an accessible guide to putting revised EYFS into practice. Dr Julian Grenier CBE is the headteacher of Sheringham Nursery School and Children’s Centre. He was the lead writer of Development Matters (2021). Caroline Vollans is a psychoanalyst and author. She writes for a wide range of early years publications and is author of Wise Words: How Susan Isaacs Changed Parenting.
The Monstrous Kind: a sweepingly romantic, atmospheric gothic fantasy (The Monstrous Kind)
by Lydia GregovicThe darkest secrets lie closest to home . . .Merrick Darling's life is better than most. As the daughter of a powerful lord, she wants for nothing - yet she will never inherit her family's Manor. Her only options are a smart marriage, or to live at the kindness of her practical and regimented elder sister, Essie. Until the day her father dies in mysterious circumstances, and Merrick is torn from her glamorous New London society life to Norland House, her family's mist-shrouded ancestral home. But the Manor has changed while Merrick has been away. Once strong and capable, Essie is withdrawn and frightened, and with good reason. The borders - their main defence against the toxic mist that has claimed much of their nation, and the deadly monsters that lurk within - have been failing for unknown reasons. When Essie goes missing, it's clear that Merrick needs help. But who can she trust when everyone seems to be scheming, and all she holds true feels like it's slipping out of her grasp?
The Monstrous Kind: a sweepingly romantic, atmospheric gothic fantasy (The Monstrous Kind)
by Lydia GregovicThe darkest secrets lie closest to home . . .Merrick Darling's life is better than most. As the daughter of a powerful lord, she wants for nothing - yet she will never inherit her family's Manor. Her only options are a smart marriage, or to live at the kindness of her practical and regimented elder sister, Essie. Until the day her father dies in mysterious circumstances, and Merrick is torn from her glamorous New London society life to Norland House, her family's mist-shrouded ancestral home. But the Manor has changed while Merrick has been away. Once strong and capable, Essie is withdrawn and frightened, and with good reason. The borders - their main defence against the toxic mist that has claimed much of their nation, and the deadly monsters that lurk within - have been failing for unknown reasons. When Essie goes missing, it's clear that Merrick needs help. But who can she trust when everyone seems to be scheming, and all she holds true feels like it's slipping out of her grasp?
The Monstrous Kind
by Lydia GregovicAn atmospheric, haunting, romantasy inspired by Jane Austen&’s Sense and Sensibility, set in Regency era England about two sisters fighting to hold on to their manor while deadly monsters prowl along its perimeters—perfect for fans of House of Salt and Sorrows and Anatomy: A Love Story.Merrick Darling&’s life as daughter of the Manor Lord of Sussex is better than most. Unlike the commoners, she is immune to the toxic fog that encroached on England generations earlier. She will never become a Phantom—one of the monstrous creatures that stalk her province&’s borders—and as long as the fires burn to hold them back, her safety is ensured. She wants for nothing, yet she will never inherit her family&’s Manor. She must marry smartly or live at the kindness of her elder sister, Essie.Everything is turned on its head, though, when Merrick&’s father dies suddenly. Torn from her New London society life of ball gowns and parties, Merrick must travel back to her childhood home, the Darling estate of Norland House, and what she finds there is bewildering. Once strong and capable, Essie is withdrawn and frightened—and with good cause. A recent string of attacks along the province&’s borders has turned their formerly bucolic countryside into a terrifying and unpredictable landscape. The fog is closing in and the fires aren&’t holding, which makes Merrick and Essie vulnerable in more ways than one. Because the Phantoms are far from the only monsters in Merrick&’s world, and the other eleven Manor Lords are always watching for weakness.Revealing her and her sister&’s current state to the rest of the Manors is out of the question, but when Essie goes missing, it&’s clear that Merrick needs help. Only, who can she trust when everyone seems to be scheming, and when all she holds true feels like it&’s slipping right out of her grasp?
Careers in Media and Film: The Essential Guide
by Georgina Gregory Ewa Mazierksa Ros J HealyWritten for students, graduates and academics from the disciplines of film, media and related subjects, and for those from other disciplines who want to break into the media, this book is a virtual career coach and an employability course all in one package. A practical handbook, it offers encouragement, advice, information and case studies to help students to make the most of the opportunities in this very competitive career world. The book can be used as a textual support for careers modules and PDP (Personal Development Planning), graduate workshops, on-line courses and as a departmental or careers library resource. Equally, it works effectively as a self-help guide to enable individuals to focus on their career / life development.
Before Dementia: 20 Questions You Need to Ask About Preventing, Preparing, Coping
by Dr. Kate Gregorevic, PhDStructured around 20 questions you need to ask to help prevent, prepare, and cope, this book is a friendly, authoritative guide for anyone facing dementia and those who care for them. Exploring why disease is a social construct just as much as a biological construct, it helps us understand what it means to live with or care for someone with dementia.How do I know if I have dementia, and how will I live with it if I do? Can people with dementia consent to sex? Can they choose euthanasia for their future selves? And can we prevent or push back its onset? Chances are you know someone with dementia, but how well do you really understand the condition? Dementia is a complex interplay of biological, social, and psychological factors, and understanding it means understanding more about society and ourselves. Approaching the topic through 20 insightful questions, geriatrician Dr. Kate Gregorevic explains the physical state of dementia, how to relate the diagnosis to real life, what questions to ask your doctor, strategies for preventing the condition, and how we can make our homes and society better for people with dementia. While this book tackles some uncomfortable questions, its purpose is to help—to prevent, to prepare, to cope and to understand—and provide you with strategies for moving forward.
The Star Book: Stargazing Throughout the Seasons in the Northern Hemisphere
by Peter GregoStargazing Throughout the Seasons in the Northern Hemisphere is an excerpt from The Star Book that guides you through the night skies in the Northern Hemisphere, through wide-angle star charts. Looking at the main constellations, stars and celestial showpieces of the northern celestial sphere, beginning with constellations around the north pole and then taking a season by season view. Most northern constellations are as familiar to today's stargazers as they were to the ancient Greeks.Everyone is interested in the stars and on a clear night astonished by them. Stargazing Throughout the Seasons in the Northern Hemisphere will answer any questions you may have when you look up into the night sky.
The Star Book: An Introduction to Stargazing and the Solar System
by Peter GregoSee the night sky like you have never seen it before with this all-encompassing guide to astronomy. Learn all there is to know about the layout of the skies, the positions of the main constellations and the names of the brightest stars, so you can become a backyard astronomer in no time at all. With easy-to-use star charts, photographs and observational drawings of objects visible from both hemispheres, The Star Book will take your understanding and enjoyment of stargazing to the next level. Whether you use binoculars or a telescope, or even if you have no optical aid at all, there are enough celestial sights to keep anyone enthralled for a lifetime. The Star Book provides a quick and simple reference to the major stars and constellations, with easy-to-use star charts, finder charts, high-quality images and observational drawings covering the key stars viewable from all over the world. Author Peter Grego, also includes a brief introduction to the history of astronomy, an easy-to-follow explanation of the life-cycle of stars, from ignition to collapse, and information about deep sky objects such as nebulae and globular clusters. This fascinating, attractive and accessible book will become a trusted resource to make sense of the night skies, and is a wonderful gift for anyone with even a passing interest in astronomy.
The Star Book: Stargazing Throughout the Seasons in the Southern Hemisphere
by Peter GregoStargazing Throughout the Seasons in the Southern Hemisphere is an excerpt from The Star Book that guides you through the night skies in the Southern Hemisphere, through wide-angle star charts. Looking at the main constellations, stars and celestial showpieces of the southern celestial sphere, beginning with constellations around the south celestial pole and then taking a season by season view. Far southern stars had to wait until the early 17th century to be mapped by European explorers.Everyone is interested in the stars and on a clear night astonished by them. Stargazing Throughout the Seasons in the Southern Hemisphere will answer any questions you may have when you look up into the night sky.
Herbal Remedies Made Simple: A Beginner's Guide to Using Plants, Herbs, and Flowers for Health and Well-Being
by Susan Gregg Stacey Dugliss-WesselmanA do-it-yourself guide to natural healing products, with instructions on choosing plants and recipes for creating alternative wellness solutions at home.Natural alternatives to traditional medicines are everywhere, even right outside your door! Herbal Remedies Made Simple is an easy and fun way to learn about the many different healing herbs that grow all around us but we seldom see. The best part: once you’re familiar with the natural healing herbs around you, Herbal Remedies Made Simple provides you with instructions on creating your own DIY herbal remedies at home.This unique guide book splits its pages in half. The top halves of the pages detail different remedies and recipes for health, personal care, and home care. The bottom pages provide reference material on the herbs used in the project above; detailing the plant’s medicinal properties and how best to work with that herb.Some of the therapeutic recipes within:Lavender cleanserRose water tonerChamomile facial exfoliantComfrey joint and bruise salveNerve tonic tinctureTeas for headaches, stress, sleep, and coldsJoint and muscle soakFully photographed and illustrated, this is the perfect guide for the beginning herbal practitioner.
The Quiet House: Reflections on the Loss of a Spouse
by Ronald J. GreerFind comfort in a message of hope and healing.The loss of a spouse is a devastating experience, but pastoral counselor Ron Greer invites readers into his own grief journey with messages of hope and healing. The Quiet House calls on the image of a home silenced by absence but also speaks about the possibility of moving forward together through the heartache of loss toward hope. Through an elegant series of personal reflections, Greer, a pastoral counselor, offers steps and reflections of healing while tending to marriage memories. Pastors may find this book a profound help and comfort for grieving members.
Hers for the Weekend
by Helena GreerReaders of Mia Sosa and Alexandria Bellefleur will love this swoony, sexy opposites-attract romcom from USA Today bestselling author Helena Greer, where the relationship may be fake, but the feelings are very real! No-nonsense Tara Sloane Chadwick is practically perfect. An impeccably mannered Southern belle, she&’s the youngest to make partner at her law firm and still friends with all her exes. However, when the woman behind her most humiliating breakup invites Tara to her wedding, Tara panics at the thought of showing up alone and impulsively declares she&’s bringing her very serious girlfriend. One issue: Tara is seriously single. Waitress and wild child Holly Siobhan Delaney may be lusting over Tara—but Tara only dates women she can marry, and Holly&’s sworn off relationships. So when Tara needs a fake girlfriend, Holly&’s eager to propose a no-strings, temporary fling. Only sharing secrets and steamy kisses show Holly the caring woman beneath Tara&’s picture-perfect exterior, tempting Holly to break her own rules. Can these two opposites trust their feelings enough to try for forever—or will their relationship go down in flames?
Maxed Out (Orca Currents)
by Daphne GreerTwelve-year-old Max just wants to play hockey. But ever since his dad died, Max's mom has been struggling. Now Max has to take his brother, Duncan, who is autistic, with him wherever he goes. Max's team is counting on him to help them win the upcoming playoff game against the Red Eagles, but one practice makes it clear that it's not safe to leave his brother unattended on the sidelines. With only a week to figure out how he can play in the big game, Max is feeling the pressure. Will he find a way to be a good teammate, a good brother and a good son, or is it all too much for one kid? This short novel is a high-interest, low-reading level book for middle-grade readers who are building reading skills, want a quick read or say they don’t like to read! The epub edition of this title is fully accessible.
Drummer Boy of John John
by Mark GreenwoodThe perfect book for aspiring young drummers, where a young boy in Trinidad discovers he can create tunes on discarded metal tins, and goes on to win the Carnival band competition.Carnival is coming, and the villagers of John John, Trinidad, are getting ready to jump up and celebrate with music, dancing, and a parade. Best of all, the Roti King has promised free rotis--tasty fried pancakes filled with chicken, herbs, and spices--for the best band in the parade. Young Winston dreams of feasting on those delicious rotis. But there's a problem: he's not in a band! Pondering his predicament as he wanders through the village junkyard, Winston makes a curious musical discovery that may be just the ticket to realizing his dream. With ingenuity and the help of his friends, Winston takes on the Carnival bands, drumming his way to victory--and to the Roti King's prized treat. Musical text and sun-drenched paintings joyously transport readers to the Caribbean in this exuberant story inspired by the early life of Winston "Spree" Simon, a pioneer in the development of the steel drum.
Employment and the Depressed Areas (Routledge Library Editions: Work & Society)
by H. Powys GreenwoodOriginally published in 1936 during the Great Depression this book analyses the efforts of the British Government to relieve the rampant unemployment in the most distressed areas and discusses why these efforts were ineffective. The book put forward a number of proposals to help ease unemployment and encourage investment in depressed areas, such as the development of trading estates, investment in transport and social services.
Elephant Touch: A Novel
by Susan R. GreenwayFor fans of Sara Pennypacker&’s Pax and Ali Benjamin&’s The Thing About Jellyfish, Elephant Touch is a contemporary middle grade novel about overcoming grief that will touch the hearts of its readers.Since the sudden loss of her mother, Quinn has been inconsolable. Her aunt brings her to volunteer at a Thai elephant sanctuary, hoping it will be a healing experience—but when Quinn learns about the previous abuse of the elephants she&’s there to help, she&’s overcome with even more grief. While crying alone by the river one day, Quinn has a magical encounter with an adult elephant. She marvels at the elephant&’s show of compassion, and they develop a strong connection. But when an orphaned baby elephant, also grieving her mother&’s death, arrives at the sanctuary in fragile health, Quinn is afraid to get involved. To help save the baby elephant&’s life, she must be courageous and use her newfound ability to connect with the elephants—not to mention accept the support of her new human friends. If she can channel her grief into action, she just might find the community and support she&’s been missing. But can she find the courage to do it?
With Liberty and Justice for Some: How the Law is Used To Destroy Equality and Protect the Powerful
by Glenn GreenwaldFrom "the most important voice to have entered the political discourse in years" (Bill Moyers), a scathing critique of the two-tiered system of justice that has emerged in AmericaFrom the nation's beginnings, the law was to be the great equalizer in American life, the guarantor of a common set of rules for all. But over the past four decades, the principle of equality before the law has been effectively abolished. Instead, a two-tiered system of justice ensures that the country's political and financial class is virtually immune from prosecution, licensed to act without restraint, while the politically powerless are imprisoned with greater ease and in greater numbers than in any other country in the world.Starting with Watergate, continuing on through the Iran-Contra scandal, and culminating with Obama's shielding of Bush-era officials from prosecution, Glenn Greenwald lays bare the mechanisms that have come to shield the elite from accountability. He shows how the media, both political parties, and the courts have abetted a process that has produced torture, war crimes, domestic spying, and financial fraud. Cogent, sharp, and urgent, this is a no-holds-barred indictment of a profoundly un-American system that sanctions immunity at the top and mercilessness for everyone else.
Elements Of Crisis Intervention: Crisis And How To Respond To Them (Hse 225 Crisis Intervention Ser.)
by James L. Greenstone Sharon C. LevitonDesigned to help in day-to-day, on-the-scene crisis intervention, ELEMENTS OF CRISIS INTERVENTION: CRISES AND HOW TO RESPOND TO THEM, 3e offers a nuts-and-bolts presentation of the information, strategies, and guidelines needed to be an effective crisis worker. A concise handbook for helping professionals who work on the front lines of crisis intervention, it is an ideal reference for crisis interveners, first responders, counselors, nurses, disaster responders, EMTs, law enforcement, human service workers, psychologists, social workers, teachers, agency directors, military, and any other professionals who encounter crisis situations.
Good Night Thoughts
by Max GreenfieldFrom actor Max Greenfield, the author of I Don't Want to Read This Book and its two companion titles, comes a sweetly funny bedtime book about anxiety and being present.What do we do when we can't fall asleep? The child in this story has racing thoughts--funny, silly, and scary--that are running on a cycle they can't stop. It's only when they begin to think to themselves: Do any of these thoughts have merit? Am I in any danger right here and now? that they are finally able to settle down.This lovely picture book is a simple but complex message about acknowledging anxiety without succumbing to it that will appeal to so many little ones (and adults) out there who find that nighttime is when their thoughts carry the most weight.
Paul Robeson
by Eloise GreenfieldCoretta Scott King Author Award Honor - American Library Association (ALA)An updated and redesigned edition of an award-winning biography of Paul Robeson, who overcame racial discrimination to become an international entertainer and civil rights activist. Includes a new introduction and afterword by the author, focusing on Robeson's legacy.Paul Robeson, born in 1898 and the son of a pastor, learned from his father to love written and spoken words, to be proud of being black, and to stand up for what he believed was right. These were the things that guided Paul throughout his life. After achieving academic and athletic success in school, Paul gained fame as a singer and an actor. His talent and his deep, rich voice won him fans and admirers worldwide. But as he traveled the globe for performances, Robeson became disturbed by the poverty and injustices he saw. In the 1940s and 1950s he began speaking out and fighting for freedom. Such activism was not tolerated, and Robeson came to be considered an enemy by the United States government. With dignity and a dynamic spirit, Paul Robeson-athlete, actor, singer, and civil rights activist-stayed true to himself and took a stand for his beliefs. A new generation of readers will soon be introduced to this courageous man.
The Luminaries: The Psychology of the Sun and Moon in the Horoscope (Seminars in Psychological Astrology #Vol. 3)
by Liz Greene Howard SasportasFrom the founders of the Centre for Psychological Astrology, the third seminar in their series for practitioners discusses sun and moon signs.Liz Greene and Howard Sasportas, psychologists, astrologers and founders of the Centre for Psychological Astrology in London continue their series of seminars with The Luminaries. In this book, they discuss the mythology and psychology of the Moon, showing its relevance as a significator of relationships. In addition, the authors explore in depth the correspondence between the Sun and the development of consciousness. The Luminaries also includes a chapter on the lunation cycle.
Once Upon a Time I Lived on Mars: Space, Exploration, and Life on Earth
by Kate GreeneWhen it comes to Mars, the focus is often on how to get there: the rockets, the engines, the fuel. But upon arrival, what will it actually be like?In 2013, Kate Greene moved to Mars. That is, along with five fellow crew members, she embarked on NASA’s first HI-SEAS mission, a simulated Martian environment located on the slopes of Mauna Loa in Hawai'i. For four months she lived, worked, and slept in an isolated geodesic dome, conducting a sleep study on her crew mates and gaining incredible insight into human behavior in tight quarters, as well as the nature of boredom, dreams, and isolation that arise amidst the promise of scientific progress and glory.In Once Upon a Time I Lived on Mars, Greene draws on her experience to contemplate humanity’s broader impulse to explore. The result is a twined story of space and life, of the standard, able-bodied astronaut and Greene’s brother’s disability, of the lag time of interplanetary correspondences and the challenges of a long-distance marriage, of freeze-dried egg powder and fresh pineapple, of departure and return. By asking what kind of wisdom humanity might take to Mars and elsewhere in the Universe, Greene has written a remarkable, wide-ranging examination of our time in space right now, as a pre-Mars species, poised on the edge, readying for launch.
As King's Companion
by Forrest GreeneJaime is the fourth son of the Prince of the Heighlands. Always an outsider in his family, Jaime seems unable to ever please his father, no matter how he tries. He longs to be a fighter, a hunter, anything other than the role his father has mapped out for him: future steward to his oldest brother.But Jaime’s set path in life is overturned when the consequences of an indiscretion on his part prove to be the final straw for his father. Infuriated, Prince Halden sends Jaime away from the Heighlands, the only home Jaime has ever known, to serve at the court of the powerful and despised king of Leilani. Alone in a much more sophisticated society than he is used to, Jaime must maneuver to find his way ... and struggle to understand his feelings about the handsome King Alder of Leilani, to whom he has sworn fealty.Alder has much on his mind. Fighting a war he can’t end and can’t afford to lose, his widowed state is of far less import. Nonetheless, his Councilors are demanding he choose a Companion -- a man to please his bed -- as is expected of a widowed Leilani king with heirs. It is a choice he is in no hurry to make. The war and losses on the battlefield are of far greater concern, and the arrival of a disgraced prince from the least loyal part of his kingdom is a minor and unwanted distraction.Until Jaime walks into his court. For the first time in his life, Alder feels a genuine surge of lust for a man. But it’s a hopeless attraction. No Heighlander would admit to wanting another man, ever.Jaime wants to fight in the war. Alder wants to make love to him. Can they both possibly get what they want?